Abstract
Microarray has revolutionized the study of molecular biology, especially the application in clinical diagnostics. When used in clinical diagnostics, microarray has to meet a high degree of reproducibility, reliability and quality in order to become a standard tool. Repeatability and reproducibility are essential for providing the best data and process control. The real challenge for microarray is, however, how to produce consistent and reliable data. The variance of microarray data is contributed by the quality of sample source, the quality of glass substrates, hybridization, and probe labeling and spotting. The quality of coated glass substrates is one of the main factors. This paper is focusing on discussing how to optimize coating conditions to improve the slide quality, consequently improve the data quality such as sensitivity and data reliability.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Brown, P.O., Botstein, D., Nat. Genet., 1999, 21:33–37.
Young, R. A., Cell, 2000, 102:9–15.
Lockhart, D. J., Winzeler, E. A., Nature, 2000, 405:827–836.
Dill, K., Stanker, L. H., and Young, C. D., J. Biochem. Biophys. Methods, 1999, 41:61–67.
Struelens, M. J., De Gheldre, Y., Deplano, A., Infect. Control Hosp. Epidemiol., 1998, 19: 565–569.
Wodicka, L., Dong, H. L., Mittmann, M., Ho, M. H., Lockhart, D. J., Saccharomyces Cerevisiae. Nat. Biotechnol., 1997, 15:1359–1367.
Cheung, V. G., Gregg, J. P., Gogolin-Ewens, K. J., Bandong, J., Stanley, C. A., Baker, L., Higgins, M. J., Nowak, N. J., Shows, T. B., Ewens, W. J., Spielman, R. S., Nature Genet., 1998, 18:225–230.
Lipshutz, R. J., Fodor, S. P., Gingeras, T. R., Lockhardt, D. J., Nature Genet., 1999, 21:20–24.
McGall, G., Labadie, J., Brock, P., Wallraff, G., Nguyen, T., Hinsberg, W., Proc. Natl Acad Sci. USA, 1996, 93:13555–13560.
Singh-Gasson, S., Green, R.D., Yeu, Y., Nelson, C., Blattner, F., Sussman, M. R., Cerrina, F., Nature Biotechnol., 1999, 17:974–978.
Milner, N., Mir, K. U., Southern, E. M., Nature Biotechnol., 1997, 15:537–541.
Proudnikov, D, Timofeev, E., Mirzabekov, A., Anal Biochem., 1998, 259:34–41.
Chrisey, L. A., Lee, G. U., O’Ferrall, C. E., Nucleic Acids Res., 1996, 24:3031–3039.
Rasmussen, S. R., Larsen, M. R., Rasmussen, S. E., Anal. Biochem., 1991, 198: 138–142.
Salo, H., Virta, P., Hakala, H., Prakash, T. P., Kawasaki, A. M., Manoharan, M., Lonnberg, H., Bioconjug. Chem., 1999, 10:815–823.
Rogers, Y. H., Jiang-Baucom, P., Huang, Z. J., Bogdanov, V., Anderson, S., Boyce-Jacino, M. T., Anal. Biochem., 1999, 266:23–30.
Cohen, G., Deutsch, J., Fineberg, J., Levine, A., Nucelic Acids Res., 1997, 25, 911–912.
Beier, M., Hoheisel, J. D., Nucelic Acids Res., 1999, 27:1970–1977.
Cheung, V. G., Morley, M., Aguilar, F., Massimi, A., Kucherlapati, R., Childs, G., Nature Genet., 1999, 21:15–19.
Morozov, V. N., Morozova, T. Y. A., Anal. Chem., 1999, 71:3110–3117.
Kothapalli, R., Yoder, S. J., Mane, S., Loughran, T. P., BMC Bioinformatics, 2002, 3, 22.
Hill, J. H., Petrucci, R. H., General Chemistry, Prentice Hall, Inc. ISBN 0-02-354481-3. 1996.
Kumar, A., Liang, Z., Nucelic Acids Res., 2001, 29:e2.
Diehl, F., Grahlmann, S., Beier, M., Hoheisel, J. D., Nucleic Acids Res. 2001, 29:e38.
Halliwell, C. M., Cass, A. E. G., Anal. Chem., 2001, 73:2476–2483.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Springer Science+Business Media, Inc.
About this chapter
Cite this chapter
Zong, Y., Wang, Y., Shi, J., Zhang, S. (2006). The Application of Novel Multi-Functional Microarray Slides for Immobilization Biomolecules. In: Xing, WL., Cheng, J. (eds) Frontiers in Biochip Technology. Springer, Boston, MA. https://doi.org/10.1007/0-387-25585-0_10
Download citation
DOI: https://doi.org/10.1007/0-387-25585-0_10
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-387-25568-2
Online ISBN: 978-0-387-25585-9
eBook Packages: EngineeringEngineering (R0)