Summary
We evaluated the possible effectiveness of genistein (GEN) as an inhibitor of mammary cancer in the ACI rat, a strain genetically susceptible to estrogen-induced mammary carcinogenesis. In this study, three-week old ACI rats were fed a phytoestrogen-free diet or a diet containing 250 ppm GEN. At nine weeks of age, the rats were implanted with 20 mg pellets containing either 2 mg 17β-estradiol (E2)/18 mg cholesterol or cholesterol alone. The appearance of palpable mammary tumors (MTs) was recorded. Our results show that E2-treated rats fed the GEN diet both prepubertally and as adults had the highest incidence of palpable MTs. E2-treated rats fed the GEN diet only prepubertally had delayed appearance of palpable MTs but the incidence quickly approached that of the rats fed GEN for the whole treatment period. Dietary GEN fed to adult E2-treated rats had the lowest incidence of MT. Rats implanted with the cholesterol pellet and fed either diet did not develop MTs.
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References
Parkin DM, Bray FI, Devesa SS (2001) Cancer burden in the year 2000. The global picture. Eur J Cancer 37:S4–66.
Hilakivi-Clarke L, Forsen T, Eriksson JG, et al (2001) Tallness and overweight during childhood have opposing effects on breast cancer risk. Br J Cancer 85:1680–1684.
Maekawa A Odashima S (1975) Spontaneous tumors in ACI/N rats. J Natl Cancer Inst 55:1437–1445.
Shull JD, Spady TJ, Snyder MC, et al (1997) Ovary-intact, but not ovariectomized female ACI rats treated with 17beta-estradiol rapidly develop mammary carcinoma. Carcinogenesis 18:1595–1601.
Harvell DM, Strecker TE, Tochacek M, et al (2000) Rat strain-specific actions of 17beta-estradiol in the mammary gland: correlation between estrogen-induced lobuloalveolar hyperplasia and susceptibility to estrogen-induced mammary cancers. Proc Natl Acad Sci USA 97:2779–2784.
Wiklund J, Wertz N, Gorski J (1981) A comparison of estrogen effects on uterine and pituitary growth and prolactin synthesis in F344 and Holtzman rats. Endocrinology 109:1700–1707.
Spady TJ, Lemus-Wilson AM, Pennington KL, et al (1998) Dietary energy restriction abolishes development of prolactin-producing pituitary tumors in Fischer 344 rats treated with 17beta-estradiol. Mol Carcinog 23:86–95.
Spady TJ, Pennington KL, McComb RD, et al (1999) Estrogen-induced pituitary tumor development in the ACI rat not inhibited by dietary energy restriction. Mol Carcinog 26:239–253.
Harvell DM, Strecker TE, Xie B, et al (2001) Diet-gene interactions in estrogen-induced mammary carcinogenesis in the ACI rat. J Nutr 131:3087S–3091S.
Harvell DM, Strecker TE, Xie B, et al (2002) Dietary energy restriction inhibits estrogen-induced mammary, but not pituitary, tumorigenesis in the ACI rat. Carcinogenesis 23:161–169.
Li SA, Weroha SJ, Tawfik O, et al (2002) Prevention of solely estrogen-induced mammary tumors in female ACI rats by tamoxifen: evidence for estrogen receptor mediation. J Endocrinol 175:297–305.
Butcher RL, Collins WE, Fugo NW (1974) Plasma concentration of LH, FSH, prolactin, progesterone and estradiol-17beta throughout the 4-day estrous cycle of the rat. Endocrinology 94:1704–1708.
Li JJ, Papa D, Davis MF, et al (2002) Ploidy differences between hormone-and chemical carcinogen-induced rat mammary neoplasms: comparison to invasive human ductal breast cancer. Mol Carcinog 33:56–65.
Wu AH, Ziegler RG, Horn-Ross PL, et al (1996) Tofu and risk of breast cancer in Asian-Americans. Cancer Epidemiol Biomarkers Prev 5:901–906.
Adlercreutz CH, Goldin BR, Gorbach SL, et al (1995) Soybean phytoestrogen intake and cancer risk. J Nutr 125:757S–770S.
Lee HP, Gourley L, Duffy SW, et al (1991) Dietary effects on breast-cancer risk in Singapore. Lancet 337:1197–1200.
Ziegler RG, Hoover RN, Pike MC, et al (1993) Migration patterns and breast cancer risk in Asian-American women. J Natl Cancer Inst 85:1819–1827.
Messina MJ, Persky V, Setchell KD, et al (1994) Soy intake and cancer risk: a review of the in vitro and in vivo data. Nutr Cancer 21:113–131.
Trock BJ, White BL, Clarke R, et al (2000) Meta-analysis of soy intake and breast cancer risk. J Nutr 130:653S–680S.
Fritz WA, Coward L, Wang J, et al (1998) Dietary genistein: perinatal mammary cancer prevention, bioavailability and toxicity testing in the rat. Carcinogenesis 19:2151–2158.
Lamartiniere CA (2002) Timing of exposure and mammary cancer risk. J Mammary Gland Biol Neoplasia 7:67–76.
Lamartiniere CA, Cotroneo MS, Fritz WA, et al (2002) Genistein chemoprevention: timing and mechanisms of action in murine mammary and prostate. J Nutr 132:552S–558S.
Gallo D, Giacomelli S, Cantelmo F, et al (2001) Chemoprevention of DMBA-induced mammary cancer in rats by dietary soy. Breast Cancer Res Treat 69:153–164.
Gallo D, Ferrandina G, Giacomelli S, et al (2002) Dietary soy modulation of biochemical parameters in DMBA-induced mammary tumors. Cancer Lett 186:43–48.
Appelt LC, Reicks MM (1999) Soy induces phase II enzymes but does not inhibit dimethylbenz[a]anthracene-induced carcinogenesis in female rats. J Nutr 129:1820–1826.
Constantinou AI, Lantvit D, Hawthorne M, et al (2001) Chemopreventive effects of soy protein and purified soy isoflavones on DMBA-induced mammary tumors in female Sprague-Dawley rats. Nutr Cancer 41:75–81.
Gotoh T, Yamada K, Yin H et al (1998) Chemoprevention of N-nitroso-N-methylurea-induced rat mammary carcinogenesis by soy foods or biochanin A. Jpn J Cancer Res 89:137–142.
Cohen LA, Zhao Z, Pittman B, et al (2000) Effect of intact and isoflavone-depleted soy protein on NMU-induced rat mammary tumorigenesis. Carcinogenesis 21:929–935.
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Turan, V.K., Reuhl, K.R., Thomas, P.E. (2005). Effect of Dietary Genistein on Estradiol-induced Mammary Carcinogenesis in the ACI Rat. In: Li, J.J., Li, S.A., Llombart-Bosch, A. (eds) Hormonal Carcinogenesis IV. Springer, Boston, MA. https://doi.org/10.1007/0-387-23761-5_40
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DOI: https://doi.org/10.1007/0-387-23761-5_40
Publisher Name: Springer, Boston, MA
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