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Novel Insights Into Protein Structure and Dynamics Utilizing the Red Edge Excitation Shift Approach

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Book cover Reviews in Fluorescence 2005

Part of the book series: Reviews in Fluorescence ((RFLU,volume 2005))

Abstract

A shift in the wavelength of maximum fluorescence emission toward higher wavelengths, caused by a corresponding shift in the excitation wavelength toward the red edge of the absorption band, is termed the red edge excitation shift (REES). This effect is mostly observed with polar fluorophores in motionally restricted media such as viscous solutions or condensed phases where the dipolar relaxation time for the solvent shell around a fluorophore is comparable to or longer than its fluorescence lifetime. REES arises from slow rates of solvent relaxation (reorientation) around an excited state fluorophore which depends on the motional restriction imposed on the solvent molecules in the immediate vicinity of the fluorophore. Utilizing this approach, it becomes possible to probe the mobility parameters of the environment itself (which is represented by the relaxing solvent molecules) using the fluorophore merely as a reporter group. Further, since the ubiquitous solvent for biological systems is water, the information obtained in such cases will come from the otherwise ‘optically silent’ water molecules. This makes REES extremely useful since hydration plays a crucial modulatory role in the formation and maintenance of organized molecular assemblies such as folded proteins in aqueous solutions and biological membranes. The application of REES as a powerful tool to monitor the organization and dynamics of a variety of soluble, cytoskeletal, and membrane-bound proteins is discussed

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    H. Raghuraman, Devaki A. Kelkar & Amitabha Chattopadhyay

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Raghuraman, H., Kelkar, D.A., Chattopadhyay, A. (2005). Novel Insights Into Protein Structure and Dynamics Utilizing the Red Edge Excitation Shift Approach. In: Geddes, C.D., Lakowicz, J.R. (eds) Reviews in Fluorescence 2005. Reviews in Fluorescence, vol 2005. Springer, Boston, MA. https://doi.org/10.1007/0-387-23690-2_9

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