Abstract
Biodegradation studies were carried out in aerobic batch systems using Benzene, Toluene and Xylene (BTX) as the sole carbon source to evaluate the versatility of a bacterial consortium taken from a domestic sewage treatment plant. This mixed microbial culture was acclimatized using individual BTX compounds and the potential capability of microbes precultured on a single substrate to degrade other compounds was studied over a wide range of BTX concentrations (∼50-500mg/L). The biodegradation pattern and growth rates ascertained experimentally in these versatility studies were compared with results obtained from individual degradation experiments studied earlier. Similar growth pattern was observed for all the substrate with inhibition occurring at higher concentrations (∼200 mg/L for Benzene and Xylene, ∼250mg/L for Toluene). Toluene was degraded more than the other substrates followed by benzene and xylene. Adaptation to a more toxic compound like benzene and xylene improved the utilization of toluene. On the other hand microbes grown on a less toxic compound (toluene), grew at a lower rate in the presence of more toxic compounds. Kinetic parameters such as μmax, KI and KS were determined using the Haldane’s and Levenspiel’s substrate inhibition models. Results from Haldane’s model were more illustrative of the experimental observations and were found to satisfactorily explain the system behavior.
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References
Alexander, M. 1977. In: Introduction to soil microbiology, Wiley, Newyork, NY, 2nd ed. pp. 467.
Alvarez, P.J., and Vogel, T.M. 1991. Substrate interact ions of benzene, toluene and para-xylene during microbial degradation by pure cultures and mixed culture aquifer slurries. Appl. Environ. Microbiol. 57, 2981–2985.
Bielefeldt, A.R., and Stensel, H.D. 1999. Modeling competitive inhibition effects during biodegradation of BTEX mixtures. Water Res. 33(3), 707–714.
Chang, M.K., Voice, T.C., and Criddle, C.S. 1993. Kinetics of competitive inhibition of cometabolism in the biodegradation of benzene, toluene and para xylene by two Pseudomonas isolates. Biotechnol. Bioeng. 41, 1057–1065.
Cozzarelli, I., Eganhous, R.P., and Baedecker, M.J. 1990. Transformation of mono aromatic hydrocarbons to organic acids in anoxic grou ndwater environment. Environ. Geol. Wat. Sci. 16, 135–141.
Haldane, J.B.S. 1930. In: Enzymes, Longmans Green and Co. Ltd. London, Great Britain.
Levenspiel, O. 1980. The Monod equation: A revisit and a generalization to product inhibition situation. Biotechnol. Bioengg. 22, 1671–1687.
Oh, Y., Shareefdeen, Z., Baltziz, B.C., and Bartha, R. 1994. Interactions between benzene, toluene and p-xylene during their biodegradation. Biotechnol. Bioeng. 44, 533–538.
Reardon, K.F., Mosteller, D.C., and Rogers, J.D.B. 2000. Biodegradation kinetics of benzene, toluene and phenol as single and mixed substrates for Pseudomonas putida F1. Biotechnol. Bioeng. 69(4), 385–400.
Schwarzenbach, R.P., Gschwend, P.M., and Imboden, D.M. 1993. In: Environmental organic chemistry, Wiley, Newyork, NY, pp. 681.
Shim, H., and Yang, S.T., 1999. Biodegradation of benzene, toluene, ethylbenzene and o-xylene by a coculture of Pseudomonas putida and Pseudomonas fluoresens immobilized in a fibrous — bed bioreactor. J. Biotechnol. 67, 99–112.
Simkins, S., and Alexander, M. 1984. Models for mineralization kinetics with the variables of substrate concentration and population density. Appl. Environ. Microbiol. 47, 1299–1306.
Smith, M.R. 1990. The biodegradation of aromatic hydrocarbons by bacteria. Biodegradation. 1, 191–206.
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Rene, E.R., Maliyekkai, S.M., Philip, L., Swaminathan, T. (2005). Substrate Versatility Studies on the Aerobic Degradation of BTX Compounds. In: Calabrese, E.J., Kostecki, P.T., Dragun, J. (eds) Contaminated Soils, Sediments and Water. Springer, Boston, MA. https://doi.org/10.1007/0-387-23079-3_9
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DOI: https://doi.org/10.1007/0-387-23079-3_9
Publisher Name: Springer, Boston, MA
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