Abstract
Three Pleurotus strains were grown in a medium containing MSWI fly ash having high concentrations of PCDD/Fs. The supernatants were collected every seven days up to 42 days, and used for the determination of lignin - and manganese-peroxidases (LIP and MnP), and laccase (LAC), and also for toxicity levels. Enzyme activities were determined by spectrophotometric assays. The toxicity levels were determined using a bioassay employing Lettuce (Latuca sativa) seeds. When 2.5 g were used, low levels of LIP, LAC e M nP were detected, regardless of the strain. When Pleurotus sp. CCB 068 was grown in medium containing 3.5 g of fly ash, high LAC activities (+/ - 20 U/L) was detected up to 7th day. Lower activities were observed for P. sajor caju 020 (+/- 15 U/L) e P. tailandia (+/- 10 U/L). P. sp. CCB 068 was the only strain showing a good decrease in the phytotoxic effect after 42 days of treatment (35.90% effect). Our results indicate that P. sp. CCB 068 produced metabolites which were less toxic to the lettuce seeds than the other two strains, and that the higher levels of LAC and MnP produced may play a role in the degradation of PCDD/Fs.
Keywords
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Assunçao, J V de; Pesquero, R.C. Dioxinas e furanos: origens e risco. Rev. Saúde Pública. v. 33(5), p. 523–530,1999.
Barr, D. P.; Aust, S. D. Mechanisms white-rot fungi use the degrade pollutants. Environmental Science & Technology. v. 28(2), p. 78–87, 1994.
Behnischi, P. A.; Hosoe, K.; Sakai, S-I. Combinatorial bio/ chemical analysis od dioxin and dioxin-like compounds in waste recycling, feed/ food, humans/ wildilife and the environment. Environment International. v.27.p.495–519. 2001.
Bumpus, J. A. Biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium. Appl. Environ. Microbiol. v. 55, p. 154–158, 1989.
Bumpus, J. A; Tien, M.; Whiight, D.; Aust, S. D. Oxidation of persistent environmental pollutants by a white-rot fungus. Science, v. 228, p. 1434–1436, 1985.
Camarero, S.; Böckle, B.; Martinez, M.J.; Martinez, A. Z. Manganese-mediated lignin degradation by Pleurotus pulmonaris. Appl. Environ. Microbiol. v.62. p. 1070–1072., 1996.
Chang, M-B; Chung, Y. T. Dioxins contents in fly ashes of MSW Incinerator in Taiwan. Chemosphere. v. 36(9), p. 1959–1968, 1998.
Clemente, A. R.; Anazawa, T. A.; Durrant, L. R. Biodegradation of polycyclic aromatic hydrocarbons by soil fungi. Brasilian Journal of Microbiology. v. 32, p. 255–261, 2001.
Domingo, J. L.; Granero, S.; Schuhmscher, M. Congener profiles of PCDD/Fs in soil and vegetation samples collected near to a municioal waste incinerator. Chemosphere. v. 43, p. 517–524, 2001.
Eggen, T. Application of fungal substrate from commercial mushroom production — Pleurotus ostreatus-for bioremediation of creosoto contaminated soil. International Biodeterioration & Biodegradation. v. 44, p. 117–126, 1999.
Eggen, T.; Majchercyk, A.. Removal of polycyclic aromatic hidrocarbons (PAH) in contaminated soi1 by white-rot fungus Pleurotus ostreatus. International Biodeterioration & Biodegradation. v. 41, p. 111–117, 1998.
Fries, G. F. A review of the significance of animal food products as potencial pathways of human exposures to dioxins. J. Anim. Sci. v.73, p. 1639–1650, 1995.
Hammel, K. E.; Kalyanaraman, B.; Kirk, T. K.. Oxidation of polycyclic aromatic hydrocarbons and dibenzo-p-dioxins by Phanerochaete chrysosporium ligninase. J. Bio. Chem. v.261. p. 16948–16952, 1986
Hatakka, A.. Lignin-modifying enzymes from selected white-rot fungi: production and role in lignin degradation. FEMS Microbiology Reviews. v. 13, p. 125–135, 1994.
Hester, R. E.; Harrison, R. M. 1994. Issues in Environmental Science and Technology, vol. 2. Waste incineration and the environment. Edujee, G. H, Bath Press.
Höfer, C. & Scholosser, D.. Novel enzymatici oxidation of Mn2+ to Mn3+ catalysed by a fungal laccase. FEBS Letters, v. 451, p. 186–190, 1999.
Hong, H. B.; Chang, Y. S.; Choi, D. S.; Park, Y. H.. Degradation of Dibenzofuran by Pseudomonas putida PH 01. Was. Res. v. 34(sn8), p. 24024–2407, 2000.
Hoogenboom, R.; Portier, L; Onstenk, C; Polman, T.; Hamers, A., Traag, W. Determinatio n of dioxins and planar PCBs in fish using the CG/MS and CALUX bioassay. Introduction of the screening approach for control purposes. Organohalogen Compound. v.45, p. 236–239., 2000.
Huang, Y.; Takada, M.; Takeda, N. Chlorobenzenes removal from Municipal solid waste incineration fly ash by surfactant-assisted column flotation. Chemosphere. v. 52, p. 735–743, 2003.
Ingol, C. T.; Hudson, H. J. (1993). Growth and Nutrition. In: The Biology of Fungi, 6 ed. Champman& Hall, London, p. 7–24.
Joshi, D. K.; Gold, M. H. Oxidation of dibenzo-p-dioxin by lignin peroxidase from the basidiomycete Phanerochaete chrysosporium. Biochemestry. v.33, p. 10969–10976, 1994.
Kao, C. M.; Chen, S. C.; Liu; J. K.; Wu, M. J. Evaluation of TCDD biodegradability under different redox conditions. Chemosphere. v. 44, p. 1447–1454, 2001.
Kasuga, K.; Nojiri, H.; Yamane, H.; Omori, T.. Genes of enzymes involved in the biodegradation of Carbazole, Dibenzofuran, Fluorene and Dibenzo-p-dioxin by bacteria. Wat. Sci. Tech. v. 36(10), p. 9–16, 1997.
Köller, G.; Möder, M.; Czihal, K. Peroxidative degradation of selected PCB: a mechanistic study. Chemosphere.v.41, p. 1827–1834, 2000.
Kubátová, A.; Erbanová, P.; Eichlerová, I.; Homolka, L.; Nerud, F.; Sasek, V. PCB congenere selective biodegradation by the white-rot fungus Pleurotus ostreatus in contaminated soil. Chemosphere. v.43, p. 207–215, 2001.
Kuwahara, M.; Glenn, J. K; Morgan, M. A; Gold, M.H Separation and characterization of two extracelullar H2O2 dependent oxidases from lygninolytic cultures of Phanerochaete chrysosporium. FEBS Lett. v. 169. p. 247–250, 1984.
Li; W.; Wu, W. Z.; Barbara, R.B., Schramm, K. W.; Kettrup, A. A new enzyme imunoassay for PCDD/F TEQ scrrening in environmental samples: comparision to micro-EROD assay and to chemical analysis. Chemosphere. v. 38(14), p. 499–506, 1999.
Mätzing, H.; Baumann, W.; Becker, B.; Jay, K.; Paur, H-R; Seifert, H. Adsorption of PCDD/F on MWI fly ash. Chemosphere. v. 42, p. 803–809, 2001.
Szklaarz, G. D.; Antibus, R. K, Sinsabaugaugh, R.L; Linkins, A. E. Production of phenoloxidases and peroxidases by wood-rotting fungi. Mycolo.v.81, p. 234–240, 1989.
Takada, S.; Nakamura, M.; Matsueda, T.; Kondo, R.; Sakai, K. Degradation of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans by the white-rot fungus Phanerochaete sordida YK-624. Appl Environm. Microbiol. v.62, p. 4323–4328, 1996.
Thomas, D.R.; Carswell, K.. S.; Georgiou, G. Mineralization of biphenyle and PCBs by white-rot fungus Phanerochaete chrysosporium. Biotech. Bioeng. v. 40: p. 1395–1402, 1992.(36)TIEN, M. and KIRK, T. K. (1988). “ Lignin peroxidase of Phanerochaete chrysosporium”. Methods Enzymol. 161: 238-249. & KIRK 1984
Van Overmiere, I.; Goeyenes, L.; Beernaert, H.; Srebrnik, S.; De Poorter, G. Bayeens, W.; Clark, G.; Chu, A., Chu, D; Morris, R.; Brown, D. A comparative study of GC-HRMS and CALUX-TEQ determinations in food samples by the Belgian Federal Ministries of Public Health and Agriculture. Organohalogen Compond. v.45p. 196–199, 2000
Valli, K.; Wariishi, H.; Gold, M.H Degradation of 2,7-diclorodibenzo-p-dioxin by the lignin-degrading basidiomycete Phanerochaete chrysosporium. J. Bacteriol. v. 174, p. 2131–2137, 1992
Wittichi, R-M. Degradation of dioxin-like compounds by microorganisms. Appl. Microbiol. Biotechnol. v. 49, p. 489–499, 1998.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 2005 Springer Science + Business Media, Inc.
About this chapter
Cite this chapter
Dos Reis- Gorny, M., Durrant, L. (2005). Ligninolytic Enzymes and Phytotoxic Effects Following Growth of Strains of Pleurotus in MSWI Fly Ash Contaminated with PCCD/Fs. In: Calabrese, E.J., Kostecki, P.T., Dragun, J. (eds) Contaminated Soils, Sediments and Water. Springer, Boston, MA. https://doi.org/10.1007/0-387-23079-3_22
Download citation
DOI: https://doi.org/10.1007/0-387-23079-3_22
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-387-23036-8
Online ISBN: 978-0-387-23079-5
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)