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Analytical Centrifugation: Looking at Aggregation in Free Solution

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Supramolecular Structure and Function 8
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References

  1. Svedberg, T. and Pedersen, K.O., 1940, The Ultracentrifuge. Oxford University Press, London and New York.

    Google Scholar 

  2. Stafford III, W.F., 1992, Boundary analysis in sedimentation transport experiments: a procedure for obtaining sedimentation coefficient distributions using the time derivative of the concentration profile. Analyt. Biochem. 203: 295–301.

    PubMed  CAS  Google Scholar 

  3. Stafford III, W.F., 1994, Methods for obtaining sedimentation coefficient distributions. In Analytical Ultracentrifugation in Biochemistry and Polymer ScienceI (Harding, S.E., Rowe, A.J. and Horton, J.C., eds), The Royal Society of Chemistry, Cambridge, UK, pp.359–393.

    Google Scholar 

  4. Philo, J.S., 2000, A method for directly fitting the time derivative of sedimentation velocity data and an alternative algorithm for calculating sedimentation coefficient distribution functions. Analyt. Biochem. 279: 151–163.

    PubMed  CAS  Google Scholar 

  5. Stott, K., Blackburn, J.M., Butler, P.J.G. and Perutz, M, 1995, Incorporation of glutamine repeats makes protein oligomerize: Implications for neurodegenerative diseases. Proc. Nat. Acad. Sci. USA 92: 6509–6513.

    PubMed  CAS  Google Scholar 

  6. Johnson, M.L. and Straume, M., 1994, Comments on the analysis of sedimentation equilibrium experiments. In Modern Analytical UltracentrifugationI (Schuster, T.M. and Laue, T.M., eds), Birkhäuser, Boston, USA, pp.37–65.

    Google Scholar 

  7. Meselson, M., Stahl, F.W. and Vinograd, J., 1957, Equilibrium sedimentation of macromolecules in density gradients. Proc. Nat. Acad. Sci. 43: 581–588.

    Article  CAS  PubMed  Google Scholar 

  8. Meselson, M. and Stahl, F.W., 1958, The replication of DNA in Escherichia coli. Proc. Nat. Acad. Sci. 44: 671–682.

    CAS  PubMed  Google Scholar 

  9. Edsall, J.T., 1943, Apparent molal volume, heat capacity, compressibility and surface tension of dipolar ions in solutions. In Proteins, amino acids and peptides as ions and dipolar ionsI (Cohn, E.J. and Edsall, J.T., eds), Reinhold Publishing Corp., New York, pp.155–176.

    Google Scholar 

  10. Laue, T.M., Shah, B.D., Ridgeway, T.M. and Pelletier, S.L., 1992, Computer-aided interpretation of analytical sedimentation data for proteins. In Analytical Ultracentrifugation in Biochemistry and Polymer ScienceI (Harding, S.E., Rowe, A.J. and Horton, J.C., eds), Royal Society of Chemistry, Cambridge, UK, pp.90–125.

    Google Scholar 

  11. Casassa, E.F. and Eisenberg, H., 1964, Thermodynamic analysis of multicomponent solutions. Adv. Prot. Chem. 19: 287–395.

    CAS  Google Scholar 

  12. Cohen, G. and Eisenberg, H., 1968, Deoxyribonucleate Solutions: Sedimentation in a Density Gradient, Partial Specific Volumes, Density and Refractive Index Increments, and Preferential Interactions. Biopolymers 6: 1077–1100.

    Article  PubMed  CAS  Google Scholar 

  13. Eisenberg, H., 2000, Analytical Ultracentrifugation in a Gibbsian perspective. Biophys. Chem. 88: 1–9.

    Article  PubMed  CAS  Google Scholar 

  14. Kratky, O., Leopold, H. and Stabinger, H., 1973, The determination of the partial specific volume of proteins by the mechanical oscillator technique. Methods In Enzymology 27: 98–110.

    Article  PubMed  CAS  Google Scholar 

  15. Cabezon, E., Butler, P.J.G., Runswick, M.J. and Walker, J.E., 2000, Modulation of the Oligomerization State of the Bovine F 1 -ATPase Inhibitor Protein, IF 1, by pH. J. Biol. Chem. 275: 25460–25464.

    PubMed  CAS  Google Scholar 

  16. Cabezon, E., Runswick, M.J., Leslie, A.G. and Walker, J.E., 2001, The structure of bovine IF 1, the regulatory subunit of mitochondrial F-ATPase. EMBO Journal 20: 6990–6996.

    Article  PubMed  CAS  Google Scholar 

  17. Cabezon, E., Arechaga, I., Butler, P.J.G. and Walker, J.E., 2000, Dimerization of bovine F 1 -ATPase by binding the inhibitor protein, IF 1. J. Biol. Chem. 275: 28353–28355.

    PubMed  CAS  Google Scholar 

  18. Yerushalmi, H., Lebendiker, M. and Schuldiner, S., 1996, Negative dominance studies demonstrate the oligomeric structure of EmrE, a multidrug antiporter from Escherichia coli. J. Biol. Chem. 271: 31044–31048.

    PubMed  CAS  Google Scholar 

  19. Rotem, D., Sal-man, N. and Schuldiner, S., 2001, In vitro monomer swapping in EmrE, a multidrug transporter from Escherichia coli, reveals that the oligomer is the functional unit. J. Biol. Chem. 276: 48243–48249.

    PubMed  CAS  Google Scholar 

  20. Muth, T.R. and Schuldiner, S., 2000, A membrane-embedded glutamate is required for ligand binding to the multidrug transporter EmrE. EMBO Journal 19: 234–240.

    Article  PubMed  CAS  Google Scholar 

  21. Tate, C.G., Kunji, E.R., Lebendiker, M. and Schuldiner, S., 2001, The projection structure of EmrE, a proton-linked multidrug transporter from Escherichia coli, at 7 A resolution. EMBO J. 20: 77–81.

    Article  PubMed  CAS  Google Scholar 

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Jonathan, P., Butler, G. (2005). Analytical Centrifugation: Looking at Aggregation in Free Solution. In: Pifat-Mrzljak, G. (eds) Supramolecular Structure and Function 8. Springer, Boston, MA. https://doi.org/10.1007/0-306-48662-8_4

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  • DOI: https://doi.org/10.1007/0-306-48662-8_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-48661-6

  • Online ISBN: 978-0-306-48662-3

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