Abstract
Various aspects of the branch of non-linear spectroscopy that is known as saturation transfer (ST) EPR are reviewed, ranging from its inception to the present day. Initial methodological development was by Hyde and Dalton, followed by the introduction into biology by Hyde and Thomas. ST-EPR is a continuous wave spectroscopy, which extends the sensitivity of conventional nitroxide EPR to the microsecond (or submillisecond) correlation time regime of rotational motion, for spin-labelled membranes and biopolymers. Equally, slow exchange processes are accessible to ST-EPR, as are the paramagnetic relaxation enhancements that are essential to site-directed spin-labelling strategies. Central to the latter are the principles of spin-label oximetry, as developed by Hyde and coworkers at the Milwaukee EPR Centre.
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Marsh, D., Horváth, L.I., Pálib, T., Livshits, V.A. (2005). Saturation Transfer Spectroscopy of Biological Membranes. In: Eaton, S.R., Eaton, G.R., Berliner, L.J. (eds) Biomedical EPR, Part B: Methodology, Instrumentation, and Dynamics. Biological Magnetic Resonance, vol 24/B. Springer, Boston, MA. https://doi.org/10.1007/0-306-48533-8_11
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DOI: https://doi.org/10.1007/0-306-48533-8_11
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