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Signature-tagged Mutagenesis of Yersinia pestis

  • Petra C.F. Oyston
  • Andrey V. Karlyshev
  • Brendan W. Wren
  • Richard W. Titball
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 529)

Keywords

Yersinia Enterocolitica Yersinia Pestis Good Output Result ureD Gene Genomic Insertion Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Hensel M., Shea JE., Gleeson C., Jones MD., Dalton E., Holden DW 1995. Simultaneous identification of bacterial virulence genes by negative selection. Science 269: 400–403PubMedGoogle Scholar
  2. Darwin AJ., Miller VL. 1999. Identification of Yersinia enterocolitica genes affecting survival in an animal host using signature-tagged transposon mutagenesis. Mol. Microbiol. 32: 51–62CrossRefPubMedGoogle Scholar
  3. Karlyshev AV., Oyston PCF., Williams K., Clark G., Titball RW., Winzeler EA., Wren BW. 2001. Application of high-density array-based signature-tagged mutagenesis to discover virulence-associated genes. Infect. Immun. 69: 7810–7819PubMedGoogle Scholar
  4. Sebbane F., Devalckenaere A., Foulon J., Carniel E., Simonet M. 2001. Silencing and reactivation of urease in Yersinia pestis id determined by one G residue at a specific position in the ureD gene. Infect. Immun. 69: 170–176CrossRefPubMedGoogle Scholar
  5. Karlyshev AV., Pallen MJ., Wren BW. 2000. Single-primer PCR procedure for rapid identification of transposon insertion sites. BioTechniques. 28: 1078–1082PubMedGoogle Scholar
  6. Khattar MM 1997. Overexpression of the hslVU operon supresses SOS-mediated inhibition of cell division in Escherichia coli. FEBS Letters. 414: 402–404CrossRefPubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Petra C.F. Oyston
    • 1
    • 2
  • Andrey V. Karlyshev
    • 1
    • 2
  • Brendan W. Wren
    • 1
    • 2
  • Richard W. Titball
    • 1
    • 2
  1. 1.Dstl, Chemical and Biological SciencesUniversity of LondonLondonUK
  2. 2.London School of Hygiene and Tropical MedicineUniversity of LondonLondonUK

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