Conclusions
Temperature seems to regulate both O-antigen gene cluster operons of Y. enterocolitica O:3 mainly during the stationary phase of growth. In exponential phase bacteria both operons are actively transcribed independent of temperature since temperature shift during exponential phase did not have signifigant effect on the luciferase activity. Dilution of stationary phase bacteria grown at RT decreases the light production very rapidly and dramatically. Our results suggest that in stationary phase Y. enterocolitica O:3 bacteria the O-antigen expression is controlled by a regulatory circuit which may include an activator at RT and/or a represser at 37°C. Further work is needed to elucidate regulatory mechanisms.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Al-Hendy, A., Toivanen, P., and Skurnik, M. (1991) The effect of growth temperature on the biosynthesis of Yersinia enterocolitica O:3 lipopolysaccharide: temperature regulates the transcription of the rfb but not of the rfa region. Microb Pathog 10: 81–86.
Pepe, J. C., Badger, J. L., and Miller, V. L. (1994) Growth phase and low pH affect the thermal regulation of the Yersinia enterocolitica inv gene. Mol Microbiol 11: 123–135.
Revell, P. A., and Miller, V. L. (2000) A chromosomally encoded regulator is required for expression of the Yersinia enterocolitica inv gene and for virulence. Mol Microbiol 35: 677–685.
Skurnik, M. (1999) Molecular genetics of Yersinia lipopolysaccharide. In Genetics of Bacterial Polysaccharides, J. Goldberg, ed. Boca Raton, FL: CRC Press, pp. 23–51.
Skurnik, M., and Toivanen, P. (1993) Yersinia enterocolitica lipopolysaccharide: genetics and virulence. Trends Microbiol 1: 148–152.
Zhang, L., Al-Hendy, A., Toivanen, P., and Skurnik, M. (1993) Genetic organization and sequence of the rfb gene cluster of Yersinia enterocolitica serotype O:3: Similarities to the dTDP-L-rhamnose biosynthesis pathway of Salmonella and to the bacterial polysaccharide transport systems. Mol Microbiol 9: 309–321.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Kluwer Academic Publishers
About this chapter
Cite this chapter
Lahtinen, P., Brzezinska, A., Skurnik, M. (2004). Temperature and Growth Phase Regulate the Transcription of the O-Antigen Gene Cluster of Yersinia enterocolitica O:3. In: Skurnik, M., Bengoechea, J.A., Granfors, K. (eds) The Genus Yersinia. Advances in Experimental Medicine and Biology, vol 529. Springer, Boston, MA. https://doi.org/10.1007/0-306-48416-1_55
Download citation
DOI: https://doi.org/10.1007/0-306-48416-1_55
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-47759-1
Online ISBN: 978-0-306-48416-2
eBook Packages: Springer Book Archive