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Characterisation of the Type III Secretion Protein YscU in Yersinia pseudotuberculosis

YscU cleavage — dispensable for TTSS but essential for survival
  • Moa Lavander
  • Lena Sundberg
  • Petra J. Edqvist
  • Scott A. Lloyd
  • Hans Wolf-Watz
  • Åke Forsberg
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 529)

Conclusions

The YscU/FlhB protein family is very highly conserved, amino acid sequence homologies between the cytoplasmic domains being particularly high. Here we investigated a feature common to YscU and FlhB, i. e. the cleavage of the proteins within the C-terminal cytoplasmic tail. We could conclude that the cleavage is not connected to TTSS regulation. Nor is it required for YscU function in Yersinia type III secretion, since the uncleavable YscU variants are able to restore Yop secretion to a yscU null mutant strain. However, if YscU is not cleaved within the cytoplasmic tail, it has a toxic effect on the bacteria. The fact that Yersinia strains expressing the uncleavable YscU point mutant proteins are seriously inhibited for growth shows that the cleavage is required for survival of the bacterium.

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References

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Moa Lavander
    • 1
  • Lena Sundberg
    • 1
    • 2
  • Petra J. Edqvist
    • 2
  • Scott A. Lloyd
    • 2
    • 3
  • Hans Wolf-Watz
    • 2
  • Åke Forsberg
    • 1
    • 2
  1. 1.Department of Medical Countermeasures, Division of NBC-DefenceSwedish Defence Research AgencyUmeåSweden
  2. 2.Department of Molecular BiologyUmeå UniversityUmeåSweden
  3. 3.Center for Vaccine Development, Department of PediatricsUniversity of Maryland School of MedicineBaltimore

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