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Mapping of Possible Laminin Binding Sites of Y. pestis Plasminogen Activator (Pla) via Phage Display

  • Orsolya Benedek
  • Judit Bene
  • Béla Melegh
  • Levente Emődy
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 529)

Summary and Conclusions

We tried to determine amino acid motifs of Y. pestis plasminogen activator (Pla) involved in laminin binding. We selected heptamer peptides using a random phage library which was tested against immobilised laminin. Two sequences seemed to inhibit Pla mediated laminin binding of E. coli and exhibited a strong laminin binding capacity revealing a competition with Pla for the same laminin binding site. The motifs are also involved in plasminogen activation because they caused fibrinolysis on fibrin films. The patterns were localised outside the putative surface-exposed loops (Kukkonen et al., 2001).

Keywords

Pattern Search Plasminogen Activation Phage Display Library Yersinia Pestis Virulence Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Kukkonen, M, Lähteenmäki, K, Suomalainen, M, Kalkkinen, N, Emödy, L, Lång, H, Korhonen, T. K. 2001. Protein regions important for plasminogen activation and inactivation of alpha 2-antiplasmin in the surface protease Pla of Yersinia pestis. Mol Microbiol, 40: 1097–1111CrossRefPubMedGoogle Scholar
  2. Lähteenmäki, K, Virkola, R, Saren, A, Emödy, L, Korhonen, T. K. 1998. Expression of plasminogen activator Pla of Yersinia pestis enhances bacterial attachment to the mammalian extracellular matrix. Infect Immun, 66:5755–5762PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Orsolya Benedek
    • 1
  • Judit Bene
    • 2
  • Béla Melegh
    • 2
  • Levente Emődy
    • 1
  1. 1.Department of Medical Microbiology and Immunology, Medical SchoolUniversity of PécsPécsHungary
  2. 2.Department of Genetics and Child Development, Medical SchoolUniversity of PécsPécsHungary

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