Conclusions
We have demonstrated that YopT recognizes the prenylated Rho GTPases (RhoA, Rac and Cdc42) in the host cell and executes a proteolytic cleavage at the C termini of the GTPases. This cleavage leads to the removal of the prenylated cysteine on the GTPases, which results in the membrane detachment of the GTPases. The model of YopT action is depicted in Figure 1. The proteolytic activity towards Rho GTPases described here is responsible for its known cytotoxicity. In addition, the proteolytic triad (C 139, H2598 and D274) is conserved in the entire YopT family of bacterial virulence factors, suggesting that the YopT defines a novel family of cysteine protease functioning in bacterial pathogenesis.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D.J., 1997, Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389–3402.
Barrett, A. J., and Rawlings, N. D., 2001, Evolutionary lines of cysteine peptidases. Biol Chem 382: 727–733.
Cornells, G. R., Boland, A., Boyd, A. P., Geuijen, C., Iriarte, M., Neyt, C., Sory, M. P., and Stainier, I., 1998, The virulence plasmid of Yersinia, an antihost genome. Microbiol Mol Biol Rev 62: 1315–1352.
Grosdent, N., Maridonneau-Parini, I., Sory, M. P., and Cornelis, G. R., 2002, Role of Yops and adhesins in resistance of Yersinia enterocolitica to phagocytosis. Infect Immun 70: 4165–4176.
Hancock, J. F., 1995, Prenylation and palmitoylation analysis. Methods Enzymol 255: 237–245.
Iriarte, M., and Cornelis, G. R., 1998, YopT, a new Yersinia Yop effector protein, affects the cytoskeleton of host cells. Mol Microbiol 29: 915–929.
Rawlings, N. D., and Barrett, A. J., 1994, Families of cysteine peptidases. Methods Enzymol 244: 461–486.
Shao, F., Merritt, P. M., Bao, Z., Innes, R. W., and Dixon, J. E., 2002, A Yersinia Effector and a Pseudomonas Avirulence Protein Define a Family of Cysteine Proteases Functioning in Bacterial Pathogenesis. Cell 109: 575–588.
Sorg, I., Goehring, U. M., Aktories, K., and Schmidt, G., 2001, Recombinant Yersinia YopT Leads to Uncoupling of RhoA-Effector Interaction. Infect Immun 69: 7535–7543.
Zhang, F. L., and Casey, P. J., 1996, Protein prenylation: molecular mechanisms and functional consequences. Annu Rev Biochem 65: 241–269.
Zumbihl, R., Aepfelbacher, M., Andor, A., Jacobi, C. A., Ruckdeschel, K., Rouot, B., and Heesemann, J., 1999, The cytotoxin YopT of Yersinia enterocolitica induces modification and cellular redistribution of the small GTP-binding protein RhoA. J Biol Chem 27: 29289–29293.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Kluwer Academic Publishers
About this chapter
Cite this chapter
Shao, F., Dixon, J.E. (2004). YopT Is a Cysteine Protease Cleaving Rho Family GTPases. In: Skurnik, M., Bengoechea, J.A., Granfors, K. (eds) The Genus Yersinia. Advances in Experimental Medicine and Biology, vol 529. Springer, Boston, MA. https://doi.org/10.1007/0-306-48416-1_14
Download citation
DOI: https://doi.org/10.1007/0-306-48416-1_14
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-47759-1
Online ISBN: 978-0-306-48416-2
eBook Packages: Springer Book Archive