Summary
The application of molecular techniques to problems in plant biology has yielded a wealth of important information. In the area of biotechnology, the past few years have seen molecular and transgenic technology applied to solving problems of reduced yield due to viral and insect attack. A natural progression from this is the utilization of these powerful techniques to the analysis of stress responses in plants. This chapter addresses the molecular biological approaches which are available to investigate the effects of environmental stress on the photosynthetic apparatus. Enormous progress has been made in understanding the molecular biology of chloroplast biogenesis but a full picture awaits the elucidation of the regulatory mechanisms which control this process. In order to illustrate how recombinant DNA methods can be used to investigate stress effects, examples have been taken from other systems. Transgenic plant technology and the application of molecular genetic analysis to mutants also have the potential to make an enormous impact on this area of research. Finally, some examples are given of situations in which stress effects on photosynthesis have begun to be investigated at the molecular level. It is only by understanding the molecular bases of these responses that we will be able to engineer crops which overcome negative effects of stress on yield.
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References
Aguan K, Sugawara K, Suzuki N and Kusano T (1991) Isolation of genes for low-temperature-induced proteins in rice by a simple subtractive method. Plant Cell Physiol 32: 1285–1289
Aguan K, Sugawara K, Suzuki N and Kusano T (1993) Low-temperature-dependent expression of a rice gene encoding a protein with a leucine-zipper motif. Mol Gen Genet 240: 1–8
Ahmad M and Cashmore AR (1993) HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor. Nature 366: 162–166
Baker NR and Nie G-Y (1994) Chilling sensitivity of photosynthesis in maize In: Bajaj YPS (ed) Biotechnology of maize, pp 465–481, Springer-Verlag, Berlin
Buzby JS, Yamada T and Tobin EM (1990) A light-regulated DNA-binding activity interacts with a conserved region of a Lemna gibba rbcS promoter. Plant Cell 2: 805–814
Catavelli L and Bartels D (1989) Cold-induced mRNAs accumulate with different kinetics in barley coleoptiles. Planta 178:184–188
Chory J (1993) Out of darkness: mutants reveal pathways controlling light-regulated development in plants. TIG 9:167–172
Christou P, Ford TL and Kofron M (1991) Production of transgenic rice (Oryza sativa L) plants from agronomically important Indica and Japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Bio/Technology 9: 957–962
Coen ES and Meyerowitz EM (1991) The war of the whorls: Genetic interactions controlling flower development. Nature 353:31–37
Cook WB and Miles D (1988) Transposon mutagenesis of nuclear photosynthetic genes in Zea mays. Photosynth Res 18: 33–59
Cushman JC, Meyer G, Michalowski CB, Schmitt JM and Bohnert HJ (1989) Salt stress leads to differential expression of two isogenes of phosphoenolpyruvate carboxylase during crassulacean acid metabolism induction in the common ice plant. Plant Cell 1: 715–725
Deng X-W, Tonkyn JC, Peter GF, Thornber JP and Gruissem W (1989) Post-transcriptional control of plastid mRNA accumulation during adaptation of chloroplasts to different light quality environments. Plant Cell 1: 645–654
DeRocher EJ and Bohnert HJ (1993) Development and environmental stress employ different mechanisms in the expression of a plant gene gamily. Plant Cell 5: 1611–1625
de Wet JR, Wood KV, DeLuca M, Helinski DR and Subramani S (1987) Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol 7: 725–737
D’Halluin K, Bonne E, Bossut M, de Beuckeleer M and Leemans J (1992) Transgenic maize plants by tissue electroporation. Plant Cell 4:1495–1505
Dunn MA, Hughes MA, Pearce RS and Jack PL (1990) Molecular characterisation of a barley gene induced by cold treatment. J Exp Bot 41: 1405–1413
Dunn MA, Hughes MA, Zhang L, Pearce RS, Quigley AS and Jack PL (1991) Nucleotide sequence and molecular analysis of the low temperature induced cereal gene, BLT4. Mol Gen Genet 229: 389–394
Dunn MA, Morris A, Jack PL and Hughes MA (1993) A low-temperature-responsive translation elongation factor 1 α from barley (Hordeum vulgare L.). Plant Mol Biol 23: 221–225
Fischhoff DA, Bowdish KS, Perlak FJ, Marrone PG, McCormick SM, Niedermeyer JG, Dean, DA, Kusano-Kretzmer K, Mayer EJ, Rochester DE, Rogers SG and Fraley RT (1987) Insect tolerant transgenic tomato plants. Bio/Technology 5: 807–813
Flavell RB (1995) Plant biotechnology R&D-the next ten years. TIBTECH 13: 313–319
Gilmartin PM, Sarokin L, Memelink J, and Chua N-H (1990) Molecular light switches for plant genes. Plant Cell 2: 369–378
Goddard NJ, Dunn MA, Zhang L, White AJ, Jack PL and Hughes MA (1993) Molecular analysis and spatial expression pattern of a low-temperature-specific barley gene, blt 101. Plant Mol Biol 23: 871–879
Green PJ, Yong M-H, Cuozzo M, Kano-Murakami Y, Silverstein P and Chua N-H (1988) Binding site requirements for pea nuclear protein factor GT-1 correlate with sequences required for light-dependent transcriptional activation of the rbcS-3A gene. EMBO J 7: 4035–4044
Gulick P and Dvorák J (1987) Gene induction and repression by salt treatment in roots of the salinity-sensitive Chines Spring wheat and the salinity-tolerant Chinese Spring x Elytrigia elongata amphiploid. Proc Natl Acad Sci USA 84: 99–103
Guy CL, Huber JLA and Huber SC (1992) Sucrose phosphate synthase and sucrose accumulation at low temperature. Plant Physiol 100: 502–508
Hahn M and Walbot V (1989) Effects of cold-treatment on protein synthesis and mRNA levels in rice leaves. Plant Physiol 91:930–938
Hajela RK, Horvath DP, Gilmour SJ and Thomashow MF (1990) Molecular cloning and expression of cor (Cold-Regulated) genes in Arabidopsis thaliana. Plant Physiol 93: 1246–1252
Horvath DP, McLarney BK and Thomashow MF (1993) Regulation of Arabidopsis thaliana L. (Heyn) cor78 in response to low temperature. Plant Physiol 103: 1047–1053
Hughes MA and Dunn MA (1990) The effect of temperature on plant growth and development. Biotechnol Genet Eng Rev 8:161–188
Hughes MA, Dunn MA, Pearce RS, White AJ and Zhang L (1992) An abscisic-acid-responsive, low temperature barley gene has homology with a maize phospholipid transfer protein. Plant Cell Environ 15: 861–865
Huner NPA, Öquist G, Hurry VM, Krol M, Falk S and Griffith M (1993) Photosynthesis, photoinhibition and low temperature acclimation in cold tolerant plants. Photosynth Res 37: 19–39
Jefferson RA (1987) Assaying chimeric genes in plants: theGUS gene fusion system. Plant Mol Biol Reporter 5: 387–405
Keegstra K, Olsen LJ and Theg SM (1989) Chloroplastic precursors and their transport across the envelope membranes. Ann Rev Plant Physiol Plant Mol Biol 40: 471–501
Kendrick RE and Nagatani A (1991) Phytochrome mutants. Plant J 1: 133–139
Klee H, Horsch R and Rogers S (1987) Agrobacterium-mediated plant transformation and its further applications to plant biology. Ann Rev Plant Physiol 38: 467–486
Kononowicz AK, Nelson DE, Singh NK, Hasegawa PM and Bressan RA (1992) Regulation of the osmotin gene promoter. Plant Cell 4: 513–524
Kuhlemeier C, Green PJ and Chua N-H (1987) Regulation of gene expression in higher plants. Plant Physiol 38: 221–257
Kurkela S and Franck M (1990) Cloning and characterization of a cold-and ABA-inducible Arabidopsis gene. Plant Mol Biol 15: 137–144
Liang P and Pardee AB (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257; 967–971
Mazur BJ (1995) Commercialising the products of plant biotechnology TIBTECH 13:319–324
McCormac AC, Wagner D, Boylan MT, Quail PH, Smith H and Whitelam GC (1993) Photoresponses of transgenic Arabidopsis seedlings expressing introduced phytochrome B-encoding cDNAs: Evidence that phytochrome A and phytochrome B have distinct photoregulatory functions. Plant J 4: 19–27
McKersie BD, Chen Y, de Beus M, Bowley SR, Bowler C, Inzé D, D’Halluin K and Botterman J (1993) Superoxide dismutase enhances tolerance of freezing stress in transgenic alfalfa (Medicago sativa L). Plant Physiol 103: 1155–1163
Michalowski CB, Olson SW, Piepenbrock M, Schmitt JM and Bohnert HJ (1989) Time course of mRNA induction elicited by salt stress in the common ice plant (Mesembryanthemum crystallinum). Plant Physiol 89: 811–816
Michalowski CB, DeRocher EJ, Bohnert HJ and Salvucci ME (1992) Phosphoribulokinase from ice plant: transcription, transcripts and protein expression during environmental stress. Photosynth Res 31: 127–138
Mohapatra SS, Wolfraim L, Poole RJ and Dhindsa RS (1989) Molecular cloning and relationship to freezing tolerance of cold-acclimation-specific genes of alfalfa. Plant Physiol 89: 375–380.
Mullet JE (1988) Chloroplast development and gene expression. Ann Rev Plant Physiol Plant Mol Biol 39: 475–502
Mullet JE (1993) Dynamic regulation of chloroplast transcription. Plant Physiol 103: 309–313
Murata N, Ishizaki-Nishizawa O, Higashi S, Hayashi H, Tasaka Y and Nishida (1992) Genetically engineered alteration in the chilling sensitivity of plants. Nature 356: 710–713
Nagy F, Kay SA and Chua N-H (1988) Gene regulation by phytochrome. TIG 4: 37–41
Neuhaus G, Bowler C, Kern R and Chua N-H (1993) Calcium/Calmodulin-dependent and-independent phytochrome signal transduction pathways. Cell 73: 937–952
Nickelsen J and Rochaix J-D (1994) Regulation of D1 and D2 synthesis. In: Baker NR and Bowyer JR (eds) Photoinhibition: from molecular mechanisms to the field, pp 179–194. Bios Scientific Publishers, Oxford.
Nie G-Y and Baker NR (1991) Modifications to thylakoid composition during development of maize leaves at low growth temperatures. Plant Physiol 95: 184–191
Nie G-Y, Robertson EJ, Fryer MJ, Leech RM and Baker NR (1995) Response of the photosynthetic apparatus in maize leaves grown at low temperatures on transfer to normal growth temperature. Plant Cell Environ 18: 1–12
Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H and Ozeki H (1986). Chloroplast gene organization deduced from complete sequence of liverwort Marchantia polymorpha chloroplast DNA. Nature 322: 572–574.
Parks BM and Quail PH (1993) hy8, a new class of Arabdopsis long hypocotyl mutants deficient in functional phytochrome A. Plant Cell 5: 39–48
Perras M and Sarhan F (1989) Synthesis of freezing tolerance proteins in leaves, crown and roots during cold acclimation of wheat. Plant Physiol 89: 577–585
Ramagopal S (1987) Differential mRNA transcription during salinity stress in barley. Proc Natl Acad Sci USA 84: 94–98
Reed JW, Nagpal P, Poole DS, Furuya M and Chory J (1993) Mutations in the gene for the red/far-red light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development. Plant Cell 5: 147–157
Robertson EJ, Baker NR and Leech RM (1993) Chloroplast thylakoid protein changes induced by low growth temperature in maize revealed by immunocytology. Plant Cell Environ 16:809–818
Rodermel SR, Abbott MS and Bogorad L (1988) Nuclear-organelle interactions: nuclear antisense gene inhibits ribulose bisphosphate carboxylase enzyme levels in transformed tobacco plants. Cell 55: 673–681
Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Sen Gupta A, Webb RP, Scott Holaday A and Allen RD (1993) Overexpression of superoxide dismutase protects plants from oxidative stress. Plant Physiol 103: 1067–1073
Sharma YK and Davis KR (1995) Isolation of a novel Arabidopsis ozone-induced cDNA by differential display. Plant Mol. Biol. 29: 91–98
Sharrock RA and Quail PH (1989) Novel phytochrome sequences in Arabidopsis thaliana: Structure, evolution and differential expression of a plant regulatory photoreceptor family. Genes Dev 3: 1745–1757
Sheen J (1993) Protein phosphatase activity is required for light-inducible gene expression in maize. EMBO J 12: 3497–3505
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng B-Y, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H and Sugiura M (1986). The complete nucleotide sequence of the tobacco chloroplast genome: Its gene organization and expression. EMBO J 5: 2043–2049.
Smith H (1990) Signal perception, differential expression within multigene families and the molecular basis of phenotypic plasticity. Plant Cell Environ 13: 585–594
Smith H and Whitelam GC (1990) Phytochrome, a family of photoreceptors with multiple physiological roles. Plant Cell Environ 13: 695–707
Smith H, Samson G and Fork DC (1993) Photosynthetic acclimation to shade: probing the role of phytochromes using photomorphogenic mutants of tomato. Plant Cell Environ 16: 929–937
Sullivan ML and Green PJ (1993) Post-transcriptional regulation of nuclear-encoded genes in higher plants: The roles of mRNA stability and translation. Plant Mol Biol 23: 1091–1104
Tavladoraki P, Benvenuto E, Trinca S, De Martinis D, Cattaneo A, and Galeffi P (1993) Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature 366: 469–472
Taylor WC (1989) Regulatory interactions between nuclear and plastid genomes. Ann Rev Plant Physiol Plant Mol Biol 40: 211–233
Thomas JC and Bohnert HJ (1993) Salt stress perception and plant growth regulators in the halophyte Mesembryanthemum crystallinum. Plant Physiol 103: 1299–1304.
Thomas JC, McElwain EF and Bohnert HJ (1992) Convergent induction of osmotic stress-responses. Plant Physiol 100:416–423
Thompson WF and White MJ (1991) Physiological and molecular studies of light-regulated nuclear genes in higher plants. Ann Rev Plant Physiol Plant Mol Biol 42: 423–466
Tobin EM and Silverthorne J (1985) Light regulation of gene expression in higher plants. Ann Rev Plant Physiol 36: 569–593.
Trewavas A and Gilroy S (1991) Signal transduction in plant cells. TIG 7:356–361
Urao T, Yamaguchi-Shinozaki K, Urao S and Shinozaki K. (1993) An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence. Plant Cell 5:1529–1539
Vasil V, Srivastava V, Castillo AM, Fromm ME and Vasil IK (1993) Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Bio/Technology 11:1553–1558
Vernon DM, Ostrem JA and Bohnert HJ (1993) Stress perception and response in a facultative halophyte: the regulation of salinity-induced genes in Mesembryanthemum crystallinum. Plant Cell Environ 16: 437–444.
Wagner D, Tepperman JM and Quail PH (1991) Over expression of phytochrome B induces a short hypocotyl phenotype in transgenic Arabidopsis. Plant Cell 3: 1275–1288
Walbot V (1992) Strategies for mutagenesis and gene cloning using transposon tagging and T-DNA insertional mutagenesis. Ann Rev Plant Physiol Plant Mol Biol 43: 49–82
Webber AN and Baker NR (1996) Control of thylakoid membrane development and assembly. In: Ort DR and Yocum C (eds) Oxygenic Photosynthesis: The Light Reactions, 41–58. Kluwer Academic Publishers, Dordrecht
Wester L, Somers DE, Clack T and Sharrock RA (1994) Transgenic complementation of the hy3 phytochrome B mutation and response to PHYB gene copy number in Arabidopsis. Plant Journal 5: 261–272
Whitelam GC, McCormac AC, Boylan MT and Quail PH (1992) Photoresponses of Arabidopsis seedlings expressing an introduced oat phyA cDNA: Persistence of etiolated plant type responses in light-grown plants. Photochem Photobiol 56:617–621
Willmitzer L (1988) The use of transgenic plants to study plant gene expression. TIG 4: 13–18
Yagamuchi-Shinozaki K and Shinozaki K (1994) A novel cis-acting element in and Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell 6; 251–264
Zhang H, Scheirer DC, Fowle WH and Goodman HM (1992) Expression of antisense or sense RNA of an ankyrin repeat-containing gene blocks chloroplast differentiation in Arabidopsis. Plant Cell 4: 1575–1588
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© 1996 Kluwer Academic Publishers
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Raines, C.A., Lloyd, J.C. (1996). Molecular Biological Approaches to Environmental Effects on Photosynthesis. In: Baker, N.R. (eds) Photosynthesis and the Environment. Advances in Photosynthesis and Respiration, vol 5. Springer, Dordrecht. https://doi.org/10.1007/0-306-48135-9_12
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DOI: https://doi.org/10.1007/0-306-48135-9_12
Publisher Name: Springer, Dordrecht
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