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Molecular Biological Approaches to Environmental Effects on Photosynthesis

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Photosynthesis and the Environment

Part of the book series: Advances in Photosynthesis and Respiration ((AIPH,volume 5))

Summary

The application of molecular techniques to problems in plant biology has yielded a wealth of important information. In the area of biotechnology, the past few years have seen molecular and transgenic technology applied to solving problems of reduced yield due to viral and insect attack. A natural progression from this is the utilization of these powerful techniques to the analysis of stress responses in plants. This chapter addresses the molecular biological approaches which are available to investigate the effects of environmental stress on the photosynthetic apparatus. Enormous progress has been made in understanding the molecular biology of chloroplast biogenesis but a full picture awaits the elucidation of the regulatory mechanisms which control this process. In order to illustrate how recombinant DNA methods can be used to investigate stress effects, examples have been taken from other systems. Transgenic plant technology and the application of molecular genetic analysis to mutants also have the potential to make an enormous impact on this area of research. Finally, some examples are given of situations in which stress effects on photosynthesis have begun to be investigated at the molecular level. It is only by understanding the molecular bases of these responses that we will be able to engineer crops which overcome negative effects of stress on yield.

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References

  • Aguan K, Sugawara K, Suzuki N and Kusano T (1991) Isolation of genes for low-temperature-induced proteins in rice by a simple subtractive method. Plant Cell Physiol 32: 1285–1289

    CAS  Google Scholar 

  • Aguan K, Sugawara K, Suzuki N and Kusano T (1993) Low-temperature-dependent expression of a rice gene encoding a protein with a leucine-zipper motif. Mol Gen Genet 240: 1–8

    Article  PubMed  CAS  Google Scholar 

  • Ahmad M and Cashmore AR (1993) HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor. Nature 366: 162–166

    Article  PubMed  CAS  Google Scholar 

  • Baker NR and Nie G-Y (1994) Chilling sensitivity of photosynthesis in maize In: Bajaj YPS (ed) Biotechnology of maize, pp 465–481, Springer-Verlag, Berlin

    Google Scholar 

  • Buzby JS, Yamada T and Tobin EM (1990) A light-regulated DNA-binding activity interacts with a conserved region of a Lemna gibba rbcS promoter. Plant Cell 2: 805–814

    Article  PubMed  CAS  Google Scholar 

  • Catavelli L and Bartels D (1989) Cold-induced mRNAs accumulate with different kinetics in barley coleoptiles. Planta 178:184–188

    Article  Google Scholar 

  • Chory J (1993) Out of darkness: mutants reveal pathways controlling light-regulated development in plants. TIG 9:167–172

    PubMed  CAS  Google Scholar 

  • Christou P, Ford TL and Kofron M (1991) Production of transgenic rice (Oryza sativa L) plants from agronomically important Indica and Japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Bio/Technology 9: 957–962

    Article  Google Scholar 

  • Coen ES and Meyerowitz EM (1991) The war of the whorls: Genetic interactions controlling flower development. Nature 353:31–37

    Article  PubMed  CAS  Google Scholar 

  • Cook WB and Miles D (1988) Transposon mutagenesis of nuclear photosynthetic genes in Zea mays. Photosynth Res 18: 33–59

    Article  CAS  Google Scholar 

  • Cushman JC, Meyer G, Michalowski CB, Schmitt JM and Bohnert HJ (1989) Salt stress leads to differential expression of two isogenes of phosphoenolpyruvate carboxylase during crassulacean acid metabolism induction in the common ice plant. Plant Cell 1: 715–725

    Article  PubMed  CAS  Google Scholar 

  • Deng X-W, Tonkyn JC, Peter GF, Thornber JP and Gruissem W (1989) Post-transcriptional control of plastid mRNA accumulation during adaptation of chloroplasts to different light quality environments. Plant Cell 1: 645–654

    Article  PubMed  CAS  Google Scholar 

  • DeRocher EJ and Bohnert HJ (1993) Development and environmental stress employ different mechanisms in the expression of a plant gene gamily. Plant Cell 5: 1611–1625

    Article  PubMed  CAS  Google Scholar 

  • de Wet JR, Wood KV, DeLuca M, Helinski DR and Subramani S (1987) Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol 7: 725–737

    PubMed  Google Scholar 

  • D’Halluin K, Bonne E, Bossut M, de Beuckeleer M and Leemans J (1992) Transgenic maize plants by tissue electroporation. Plant Cell 4:1495–1505

    Article  PubMed  CAS  Google Scholar 

  • Dunn MA, Hughes MA, Pearce RS and Jack PL (1990) Molecular characterisation of a barley gene induced by cold treatment. J Exp Bot 41: 1405–1413

    CAS  Google Scholar 

  • Dunn MA, Hughes MA, Zhang L, Pearce RS, Quigley AS and Jack PL (1991) Nucleotide sequence and molecular analysis of the low temperature induced cereal gene, BLT4. Mol Gen Genet 229: 389–394

    Article  PubMed  CAS  Google Scholar 

  • Dunn MA, Morris A, Jack PL and Hughes MA (1993) A low-temperature-responsive translation elongation factor 1 α from barley (Hordeum vulgare L.). Plant Mol Biol 23: 221–225

    Article  PubMed  CAS  Google Scholar 

  • Fischhoff DA, Bowdish KS, Perlak FJ, Marrone PG, McCormick SM, Niedermeyer JG, Dean, DA, Kusano-Kretzmer K, Mayer EJ, Rochester DE, Rogers SG and Fraley RT (1987) Insect tolerant transgenic tomato plants. Bio/Technology 5: 807–813

    Article  CAS  Google Scholar 

  • Flavell RB (1995) Plant biotechnology R&D-the next ten years. TIBTECH 13: 313–319

    CAS  Google Scholar 

  • Gilmartin PM, Sarokin L, Memelink J, and Chua N-H (1990) Molecular light switches for plant genes. Plant Cell 2: 369–378

    Article  PubMed  CAS  Google Scholar 

  • Goddard NJ, Dunn MA, Zhang L, White AJ, Jack PL and Hughes MA (1993) Molecular analysis and spatial expression pattern of a low-temperature-specific barley gene, blt 101. Plant Mol Biol 23: 871–879

    Article  PubMed  CAS  Google Scholar 

  • Green PJ, Yong M-H, Cuozzo M, Kano-Murakami Y, Silverstein P and Chua N-H (1988) Binding site requirements for pea nuclear protein factor GT-1 correlate with sequences required for light-dependent transcriptional activation of the rbcS-3A gene. EMBO J 7: 4035–4044

    PubMed  CAS  Google Scholar 

  • Gulick P and Dvorák J (1987) Gene induction and repression by salt treatment in roots of the salinity-sensitive Chines Spring wheat and the salinity-tolerant Chinese Spring x Elytrigia elongata amphiploid. Proc Natl Acad Sci USA 84: 99–103

    Article  PubMed  CAS  Google Scholar 

  • Guy CL, Huber JLA and Huber SC (1992) Sucrose phosphate synthase and sucrose accumulation at low temperature. Plant Physiol 100: 502–508

    PubMed  CAS  Google Scholar 

  • Hahn M and Walbot V (1989) Effects of cold-treatment on protein synthesis and mRNA levels in rice leaves. Plant Physiol 91:930–938

    PubMed  CAS  Google Scholar 

  • Hajela RK, Horvath DP, Gilmour SJ and Thomashow MF (1990) Molecular cloning and expression of cor (Cold-Regulated) genes in Arabidopsis thaliana. Plant Physiol 93: 1246–1252

    PubMed  CAS  Google Scholar 

  • Horvath DP, McLarney BK and Thomashow MF (1993) Regulation of Arabidopsis thaliana L. (Heyn) cor78 in response to low temperature. Plant Physiol 103: 1047–1053

    Article  PubMed  CAS  Google Scholar 

  • Hughes MA and Dunn MA (1990) The effect of temperature on plant growth and development. Biotechnol Genet Eng Rev 8:161–188

    CAS  Google Scholar 

  • Hughes MA, Dunn MA, Pearce RS, White AJ and Zhang L (1992) An abscisic-acid-responsive, low temperature barley gene has homology with a maize phospholipid transfer protein. Plant Cell Environ 15: 861–865

    Article  CAS  Google Scholar 

  • Huner NPA, Öquist G, Hurry VM, Krol M, Falk S and Griffith M (1993) Photosynthesis, photoinhibition and low temperature acclimation in cold tolerant plants. Photosynth Res 37: 19–39

    Article  CAS  Google Scholar 

  • Jefferson RA (1987) Assaying chimeric genes in plants: theGUS gene fusion system. Plant Mol Biol Reporter 5: 387–405

    Article  CAS  Google Scholar 

  • Keegstra K, Olsen LJ and Theg SM (1989) Chloroplastic precursors and their transport across the envelope membranes. Ann Rev Plant Physiol Plant Mol Biol 40: 471–501

    Article  CAS  Google Scholar 

  • Kendrick RE and Nagatani A (1991) Phytochrome mutants. Plant J 1: 133–139

    Article  Google Scholar 

  • Klee H, Horsch R and Rogers S (1987) Agrobacterium-mediated plant transformation and its further applications to plant biology. Ann Rev Plant Physiol 38: 467–486

    Article  CAS  Google Scholar 

  • Kononowicz AK, Nelson DE, Singh NK, Hasegawa PM and Bressan RA (1992) Regulation of the osmotin gene promoter. Plant Cell 4: 513–524

    Article  PubMed  CAS  Google Scholar 

  • Kuhlemeier C, Green PJ and Chua N-H (1987) Regulation of gene expression in higher plants. Plant Physiol 38: 221–257

    CAS  Google Scholar 

  • Kurkela S and Franck M (1990) Cloning and characterization of a cold-and ABA-inducible Arabidopsis gene. Plant Mol Biol 15: 137–144

    Article  PubMed  CAS  Google Scholar 

  • Liang P and Pardee AB (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257; 967–971

    PubMed  CAS  Google Scholar 

  • Mazur BJ (1995) Commercialising the products of plant biotechnology TIBTECH 13:319–324

    CAS  Google Scholar 

  • McCormac AC, Wagner D, Boylan MT, Quail PH, Smith H and Whitelam GC (1993) Photoresponses of transgenic Arabidopsis seedlings expressing introduced phytochrome B-encoding cDNAs: Evidence that phytochrome A and phytochrome B have distinct photoregulatory functions. Plant J 4: 19–27

    Article  CAS  Google Scholar 

  • McKersie BD, Chen Y, de Beus M, Bowley SR, Bowler C, Inzé D, D’Halluin K and Botterman J (1993) Superoxide dismutase enhances tolerance of freezing stress in transgenic alfalfa (Medicago sativa L). Plant Physiol 103: 1155–1163

    Article  PubMed  CAS  Google Scholar 

  • Michalowski CB, Olson SW, Piepenbrock M, Schmitt JM and Bohnert HJ (1989) Time course of mRNA induction elicited by salt stress in the common ice plant (Mesembryanthemum crystallinum). Plant Physiol 89: 811–816

    PubMed  CAS  Google Scholar 

  • Michalowski CB, DeRocher EJ, Bohnert HJ and Salvucci ME (1992) Phosphoribulokinase from ice plant: transcription, transcripts and protein expression during environmental stress. Photosynth Res 31: 127–138

    Article  CAS  Google Scholar 

  • Mohapatra SS, Wolfraim L, Poole RJ and Dhindsa RS (1989) Molecular cloning and relationship to freezing tolerance of cold-acclimation-specific genes of alfalfa. Plant Physiol 89: 375–380.

    PubMed  CAS  Google Scholar 

  • Mullet JE (1988) Chloroplast development and gene expression. Ann Rev Plant Physiol Plant Mol Biol 39: 475–502

    Article  CAS  Google Scholar 

  • Mullet JE (1993) Dynamic regulation of chloroplast transcription. Plant Physiol 103: 309–313

    Article  PubMed  CAS  Google Scholar 

  • Murata N, Ishizaki-Nishizawa O, Higashi S, Hayashi H, Tasaka Y and Nishida (1992) Genetically engineered alteration in the chilling sensitivity of plants. Nature 356: 710–713

    Article  CAS  Google Scholar 

  • Nagy F, Kay SA and Chua N-H (1988) Gene regulation by phytochrome. TIG 4: 37–41

    PubMed  CAS  Google Scholar 

  • Neuhaus G, Bowler C, Kern R and Chua N-H (1993) Calcium/Calmodulin-dependent and-independent phytochrome signal transduction pathways. Cell 73: 937–952

    Article  PubMed  CAS  Google Scholar 

  • Nickelsen J and Rochaix J-D (1994) Regulation of D1 and D2 synthesis. In: Baker NR and Bowyer JR (eds) Photoinhibition: from molecular mechanisms to the field, pp 179–194. Bios Scientific Publishers, Oxford.

    Google Scholar 

  • Nie G-Y and Baker NR (1991) Modifications to thylakoid composition during development of maize leaves at low growth temperatures. Plant Physiol 95: 184–191

    PubMed  CAS  Google Scholar 

  • Nie G-Y, Robertson EJ, Fryer MJ, Leech RM and Baker NR (1995) Response of the photosynthetic apparatus in maize leaves grown at low temperatures on transfer to normal growth temperature. Plant Cell Environ 18: 1–12

    Article  CAS  Google Scholar 

  • Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H and Ozeki H (1986). Chloroplast gene organization deduced from complete sequence of liverwort Marchantia polymorpha chloroplast DNA. Nature 322: 572–574.

    Article  CAS  Google Scholar 

  • Parks BM and Quail PH (1993) hy8, a new class of Arabdopsis long hypocotyl mutants deficient in functional phytochrome A. Plant Cell 5: 39–48

    Article  PubMed  CAS  Google Scholar 

  • Perras M and Sarhan F (1989) Synthesis of freezing tolerance proteins in leaves, crown and roots during cold acclimation of wheat. Plant Physiol 89: 577–585

    PubMed  CAS  Google Scholar 

  • Ramagopal S (1987) Differential mRNA transcription during salinity stress in barley. Proc Natl Acad Sci USA 84: 94–98

    Article  PubMed  CAS  Google Scholar 

  • Reed JW, Nagpal P, Poole DS, Furuya M and Chory J (1993) Mutations in the gene for the red/far-red light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development. Plant Cell 5: 147–157

    Article  PubMed  CAS  Google Scholar 

  • Robertson EJ, Baker NR and Leech RM (1993) Chloroplast thylakoid protein changes induced by low growth temperature in maize revealed by immunocytology. Plant Cell Environ 16:809–818

    Article  CAS  Google Scholar 

  • Rodermel SR, Abbott MS and Bogorad L (1988) Nuclear-organelle interactions: nuclear antisense gene inhibits ribulose bisphosphate carboxylase enzyme levels in transformed tobacco plants. Cell 55: 673–681

    Article  PubMed  CAS  Google Scholar 

  • Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Sen Gupta A, Webb RP, Scott Holaday A and Allen RD (1993) Overexpression of superoxide dismutase protects plants from oxidative stress. Plant Physiol 103: 1067–1073

    CAS  Google Scholar 

  • Sharma YK and Davis KR (1995) Isolation of a novel Arabidopsis ozone-induced cDNA by differential display. Plant Mol. Biol. 29: 91–98

    Article  PubMed  CAS  Google Scholar 

  • Sharrock RA and Quail PH (1989) Novel phytochrome sequences in Arabidopsis thaliana: Structure, evolution and differential expression of a plant regulatory photoreceptor family. Genes Dev 3: 1745–1757

    PubMed  CAS  Google Scholar 

  • Sheen J (1993) Protein phosphatase activity is required for light-inducible gene expression in maize. EMBO J 12: 3497–3505

    PubMed  CAS  Google Scholar 

  • Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng B-Y, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H and Sugiura M (1986). The complete nucleotide sequence of the tobacco chloroplast genome: Its gene organization and expression. EMBO J 5: 2043–2049.

    PubMed  CAS  Google Scholar 

  • Smith H (1990) Signal perception, differential expression within multigene families and the molecular basis of phenotypic plasticity. Plant Cell Environ 13: 585–594

    Article  CAS  Google Scholar 

  • Smith H and Whitelam GC (1990) Phytochrome, a family of photoreceptors with multiple physiological roles. Plant Cell Environ 13: 695–707

    Article  CAS  Google Scholar 

  • Smith H, Samson G and Fork DC (1993) Photosynthetic acclimation to shade: probing the role of phytochromes using photomorphogenic mutants of tomato. Plant Cell Environ 16: 929–937

    Article  CAS  Google Scholar 

  • Sullivan ML and Green PJ (1993) Post-transcriptional regulation of nuclear-encoded genes in higher plants: The roles of mRNA stability and translation. Plant Mol Biol 23: 1091–1104

    Article  PubMed  CAS  Google Scholar 

  • Tavladoraki P, Benvenuto E, Trinca S, De Martinis D, Cattaneo A, and Galeffi P (1993) Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature 366: 469–472

    Article  PubMed  CAS  Google Scholar 

  • Taylor WC (1989) Regulatory interactions between nuclear and plastid genomes. Ann Rev Plant Physiol Plant Mol Biol 40: 211–233

    Article  CAS  Google Scholar 

  • Thomas JC and Bohnert HJ (1993) Salt stress perception and plant growth regulators in the halophyte Mesembryanthemum crystallinum. Plant Physiol 103: 1299–1304.

    PubMed  CAS  Google Scholar 

  • Thomas JC, McElwain EF and Bohnert HJ (1992) Convergent induction of osmotic stress-responses. Plant Physiol 100:416–423

    Article  PubMed  CAS  Google Scholar 

  • Thompson WF and White MJ (1991) Physiological and molecular studies of light-regulated nuclear genes in higher plants. Ann Rev Plant Physiol Plant Mol Biol 42: 423–466

    Article  CAS  Google Scholar 

  • Tobin EM and Silverthorne J (1985) Light regulation of gene expression in higher plants. Ann Rev Plant Physiol 36: 569–593.

    CAS  Google Scholar 

  • Trewavas A and Gilroy S (1991) Signal transduction in plant cells. TIG 7:356–361

    PubMed  CAS  Google Scholar 

  • Urao T, Yamaguchi-Shinozaki K, Urao S and Shinozaki K. (1993) An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence. Plant Cell 5:1529–1539

    Article  PubMed  CAS  Google Scholar 

  • Vasil V, Srivastava V, Castillo AM, Fromm ME and Vasil IK (1993) Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Bio/Technology 11:1553–1558

    Google Scholar 

  • Vernon DM, Ostrem JA and Bohnert HJ (1993) Stress perception and response in a facultative halophyte: the regulation of salinity-induced genes in Mesembryanthemum crystallinum. Plant Cell Environ 16: 437–444.

    Article  CAS  Google Scholar 

  • Wagner D, Tepperman JM and Quail PH (1991) Over expression of phytochrome B induces a short hypocotyl phenotype in transgenic Arabidopsis. Plant Cell 3: 1275–1288

    Article  PubMed  CAS  Google Scholar 

  • Walbot V (1992) Strategies for mutagenesis and gene cloning using transposon tagging and T-DNA insertional mutagenesis. Ann Rev Plant Physiol Plant Mol Biol 43: 49–82

    Article  CAS  Google Scholar 

  • Webber AN and Baker NR (1996) Control of thylakoid membrane development and assembly. In: Ort DR and Yocum C (eds) Oxygenic Photosynthesis: The Light Reactions, 41–58. Kluwer Academic Publishers, Dordrecht

    Google Scholar 

  • Wester L, Somers DE, Clack T and Sharrock RA (1994) Transgenic complementation of the hy3 phytochrome B mutation and response to PHYB gene copy number in Arabidopsis. Plant Journal 5: 261–272

    Article  PubMed  CAS  Google Scholar 

  • Whitelam GC, McCormac AC, Boylan MT and Quail PH (1992) Photoresponses of Arabidopsis seedlings expressing an introduced oat phyA cDNA: Persistence of etiolated plant type responses in light-grown plants. Photochem Photobiol 56:617–621

    CAS  Google Scholar 

  • Willmitzer L (1988) The use of transgenic plants to study plant gene expression. TIG 4: 13–18

    PubMed  CAS  Google Scholar 

  • Yagamuchi-Shinozaki K and Shinozaki K (1994) A novel cis-acting element in and Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell 6; 251–264

    Article  Google Scholar 

  • Zhang H, Scheirer DC, Fowle WH and Goodman HM (1992) Expression of antisense or sense RNA of an ankyrin repeat-containing gene blocks chloroplast differentiation in Arabidopsis. Plant Cell 4: 1575–1588

    Article  PubMed  CAS  Google Scholar 

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© 1996 Kluwer Academic Publishers

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Raines, C.A., Lloyd, J.C. (1996). Molecular Biological Approaches to Environmental Effects on Photosynthesis. In: Baker, N.R. (eds) Photosynthesis and the Environment. Advances in Photosynthesis and Respiration, vol 5. Springer, Dordrecht. https://doi.org/10.1007/0-306-48135-9_12

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  • DOI: https://doi.org/10.1007/0-306-48135-9_12

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-4316-5

  • Online ISBN: 978-0-306-48135-2

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