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Structure Analysis of Single Crystals of Photosystem I by X-Ray, EPR and ENDOR: A Short Status Report

  • H. T. Witt
Part of the Advances in Photosynthesis and Respiration book series (AIPH, volume 4)

Summary

Photosystem I purified from the thermophilic cyanobacterium Synechococcus elongatus has been crystallized. The first structural model for this reaction center was based on an electron density map at 6 Å resolution. Now, with improved crystals, a new data collection at higher structural resolution is available. This status report outlines some results on the present state of X-ray structure analysis of Photosystem I at 4.5 Å resolution. Single crystals of Photosystem I have also been analyzed by magnetic resonance spectroscopy (EPR and ENDOR). The results, presented here, provide new information on the structure of the primary electron donor (P700) and on the structures of the terminal electron acceptors, the iron-sulfur clusters FA and FB.

Keywords

Hyperfine Coupling Photosynthetic Reaction Center ENDOR Spectrum Primary Electron Donor Lumenal Side 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Adman ET, Sieker LC and Jensen LH (1973) The structure of a bacterial ferredoxin. J Biol Chem 248: 3987–3996PubMedGoogle Scholar
  2. Adman ET, Sieker LC and Jensen LH (1976) Structure of Peptococcus aerogenes ferredoxin. Refinement at 2 Å resolution. J Biol Chem 251: 3801–3806PubMedGoogle Scholar
  3. Allen JP, Feher G, Yeates TO, Komiya H and Rees DC (1987) Structure of the reaction center from Rhodobacter sphaeroides R-26: The cofactors. Proc Natl Acad Sci USA 84: 5730–573PubMedGoogle Scholar
  4. Bittl R, van der Est A, Fuechsle G, Lubitz W, Stehlik D (1992) Transient EPR of photosynthetic reaction centers: P+Q+ in Zn-substituted Rb. sphaeroides and Photosystem I. In: Murata N (ed) Research in Photosynthesis, Vol I, pp 461–464. Kluwer, DordrechtGoogle Scholar
  5. Bock CH, van der Est A, Brettel K, Stehlik D (1989) Nanosecond electron transfer kinetics in Photosystem I as obtained from transient EPR at room temperature. FEBS Lett 247: 91–96CrossRefGoogle Scholar
  6. Boekema EJ, Dekker JP, van Heel MG, Rögner M, Saenger W, Witt I and Witt HT (1987) Evidence for a trimeric organization of the Photosystem I complex from the thermophilic cyanobacterium Synechococcus sp. FEBS Lett 217: 283–286CrossRefGoogle Scholar
  7. Brettel K, Siekmann I, Fromme P, van der Est A and Stehlik D (1992) Low-temperature EPR on single crystals of Photosystem I: Study of the iron-sulfur-center FA Biochim Biophys Acta 1098: 266–270Google Scholar
  8. Budil DE and Thurnauer MC (1991) The chlorophyll triplet state as a probe of structure and function in photosynthesis. Biochim Biophys Acta 1057: 1–41PubMedGoogle Scholar
  9. Chitnis VP, Quian X, Lian Y, Golbeck JH, Nakamoto H, Dian-Lin X and Chitnis PR (1993) Target inactivation of the gene psaL encoding a subunit of Photosystem I of the cyanobacterium Synechocystis sp. PCC 6803. J Biol Chem 268: 11678–11684PubMedGoogle Scholar
  10. Deisenhofer J and Michel H (1989) High resolution structures of photosynthetic reaction centers. EMBO J 8: 2149–2170PubMedGoogle Scholar
  11. Deisenhofer J, Epp O, Miki K, Huber R and Michel H (1985) Structure of the protein subunits in the photosynthetic reaction centers of Rhodopseudomonas viridis at 3 Å resolution. Nature 318: 618–624CrossRefGoogle Scholar
  12. Döring, G, Bailey JL, Kreutz, W, Weikard, J and Witt HT (1968) The action of two chlorophyll-a1-molecules in light reaction I of photosynthesis. Naturwiss 55: 219–224PubMedGoogle Scholar
  13. Fromme P, Schubert WD, Krauβ N (1994) Structure of Photosystem I: Suggestions on the docking sites for plastocyanin, ferredoxin and the coordination of P700. Biochim Biophys Acta 1187: 99–105Google Scholar
  14. Fuechsle G, Bittl R, van der Est A, Lubitz W, Stehlik D (1993) Transient EPR spectroscopy on perdeuterated Zn-substituted reaction centers of Rhodobacter sphaeroides R-26. Biochim Biophys Acta 1142: 23–35Google Scholar
  15. Golbeck JH (1994) Photosystem I in cyanobacteria. In: Bryant DA (ed) The Molecular Biology of Cyanobacteria, pp 319–360. Kluwer Academic Publishers, DordrechtGoogle Scholar
  16. Golbeck JH and Bryant DA (1991) Photosystem I. In: Lee CP (ed) Current Topics in Bioenergetics; Vol 16, pp 83–177, Academic Press, New YorkGoogle Scholar
  17. Guigliarelli B, Guillaussier J, More C, Sétif P, Bottin H, Bertrand P (1993) Structural organization of the iron-sulfur centers in Synechocystis 6803 Photosystem I. J Biol Chem 268: 900–908PubMedGoogle Scholar
  18. Hladik I and Sofrova D (1991) Does the trimeric form of Photosystem I reaction center of cyanobacteria in vivo exist? Photosyn Res 29: 171–175Google Scholar
  19. Kamlowski A, van der Est P, Fromme P and Stehlik D (1995) Structural organization of the acceptors, A1, Fx. FA and FB in Photosystem I from EPR in solution and single crystals. In: Mathis P (ed) Photosynthesis: From Light to Biosphere, Vol II, pp 29–34. Kluwer Academic Publishers, DordrechtGoogle Scholar
  20. β H (1995) Die Struktur des primären Donators P700 in Photosystem I. Thesis, TU BerlinGoogle Scholar
  21. β H, Fromme P, Witt HT and Lubitz W (1994) 1H ENDOR and 14N ESEEM in single crystals of Photosystem I from Synechococcus elongatus. Biophys J 66: A228Google Scholar
  22. Krauβ N, Hinrichs W, Witt I, Fromme P, Pritzkow W, Dauter Z, Betzel Ch, Wilson KS, Witt HT and Saenger W (1993) Three dimensional structure of system I of photosynthesis at 6 Å resolution. Nature 361: 326–331Google Scholar
  23. Kühlbrandt W, Da Neng W and Fujiyoshi Y (1994) Atomic model of plant light-harvesting complex by electron crystallography. Nature 367: 614–621PubMedGoogle Scholar
  24. Lubitz W (1991) EPR and ENDOR studies of chlorophyll cation and anion radicals. In: Scheer H (ed) Chlorophylls, pp 903–944, CRC Press, Boca RatonGoogle Scholar
  25. Mathis P (1990) Compared structure of plant and bacterial photosynthetic reaction centers. Evolutionary implications. Biochim Biophys Acta 1018: 163–167Google Scholar
  26. McDermott G, Prince SM, Freer AA, Hawthornthwaite-Lawless AM, Papiz MZ, Cogdell RJ and Isaacs NW (1995) Crystal structure of an integral membrane light-harvesting complex from photosynthetic bacteria. Nature 374: 517–521CrossRefGoogle Scholar
  27. Mühlenhoff U (1991) Zur Primär-und Quartiärstruktur des Photosystem I aus dem thermophilen Cyanobakterium Synechococcus sp. Thesis, TU BerlinGoogle Scholar
  28. Mühlenhoff U, Haehnel W, Witt HT and Herrmann RG (1993) Genes encoding eleven subunits of Photosystem I from the thermophilic cyanobacterium Synechococcus sp. GENE 127: 71–78PubMedGoogle Scholar
  29. Nitschke W, Feiler U, Lockau W and Hauska G (1987) The photosystem of the green sulfur bacterium Chlorobium limicola contains two early electron acceptors similar to Photosystem I. FEBS Lett 218: 283–286CrossRefGoogle Scholar
  30. Norris JR, Uphaus RA, Crespi HL and Katz JJ (1971) Electron spin resonance of chlorophyll and the origin of signal I in photosynthesis. Proc Natl Acad Sci 68: 625–628PubMedGoogle Scholar
  31. Oh-Oka H, Takahashi Y, Kuriyama K, Saeki K and Matsubara H (1988) The protein responsible for center A/B in spinach Photosystem I: Isolation with iron-sulfur cluster(s) and complete sequence analysis. J Biochem 103: 962–968PubMedGoogle Scholar
  32. O’Malley PJ and Babcock GT (1984) Electron nuclear double resonance evidence supporting a monomeric nature for P700* in spinach chloroplasts. Proc Natl Acad Sci USA 81: 1098–1101Google Scholar
  33. Rögner M, Mühlenhoff U, Boekema EJ and Witt HT (1990) Mono-, di and trimeric PS I reaction center complexes isolated from the thermophilic cyanobacterium Synechococcus sp.: Size, shape and activity. Biochim Biophys Acta 1015: 415–424Google Scholar
  34. Rutherford AW and Sétif P (1990) Orientation of P700, the primary electron donor of Photosystem I. Biochim Biophys Acta 1019: 128–132Google Scholar
  35. Schatz GH and Witt HT (1984) Extraction and characterization of oxygen-evolving Photosystem II complexes from a thermophilic cyanobacterium Synechococcus sp. Photobiochem Photobiophys 7: 1–4Google Scholar
  36. Schubert WD, Klukas O, Krauβ N, Saenger W, Fromme P and Witt HT (1995) Present state of the crystal structure analysis of Photosystem I at 4.5 Å resolution. In: Mathis P (ed) Photosynthesis: From Light to Biosphere, Vol II, pp 3–10. Kluwer Academic Publishers, DordrechtGoogle Scholar
  37. Shubin VV, Tsuprun VL, Bezsmertnaya IN and Karapetyan NV (1993) Trimeric forms of the Photosystem I reaction center complex pre-exist in the membranes of cyanobacterium Spirulina platensis. FEBS Lett 334: 79–82PubMedCrossRefGoogle Scholar
  38. Sieckmann I, Brettel K, Bock C, van der Est A, Stehlik D (1993) Transient EPR of the triplet state of P700 in Photosystem I: Evidence for triplet delocalization at room temperature. Biochemistry 32: 4842–4847PubMedCrossRefGoogle Scholar
  39. Snyder SW and Thurnauer MC (1993) Electron spin polarization in photosynthetic reaction centers. In: Deisenhofer J and Norris JR (eds) The Photosynthetic Reaction Center, pp 285–330, Academic Press, New YorkGoogle Scholar
  40. Stehlik D, Bock CH and Petersen J (1989) Anisotropic electron spin polarization of correlated spin pairs in photosynthetic reaction centers. J Phys Chem 93: 1612–1619CrossRefGoogle Scholar
  41. Trebst A (1986) The topology of the plastoquinone and herbicide binding peptides of Photosystem II in the thylakoid membrane. Z. Naturforsch. 41c: 240–245Google Scholar
  42. van der Est A, Bittl R, Abresch EC, Lubitz W, Stehlik D (1993) Transient EPR Centers of Rhodobacter sphaeroides R-26. Chem Phys Lett 212: 561–568Google Scholar
  43. Witt HT (1987) Examples for the cooperation of photons, excitons, electrons, electric fields and protons in the photosynthesis membrane. New J Chem 11: 91–101Google Scholar
  44. Witt I, Witt HT, Gerken S, Saenger W, Dekker JP and Rögner M (1987) Crystallization of reaction center I of photosynthesis. FEBS Lett 221: 260–264CrossRefGoogle Scholar
  45. Witt I, Witt HT, DiFiore D, Rögner M, Hinrichs W, Saenger W, Granzin J, Betzel Ch and Dauter Z (1988) X-Ray characterization of single crystals of the reaction center I of water splitting photosynthesis, Ber Bunsenges Phys Chem 92: 1503–1506Google Scholar
  46. Witt HT, Krauβ H, Hinrichs W, Witt I, Fromme P and Saenger W (1992) Three-dimensional crystals of Photosystem I from Synechococcus sp. and X-ray structure analysis at 6 Å resolution. In: Murata N (ed) Research in Photosynthesis, Vol I, pp 521–528, Kluwer, DordrechtGoogle Scholar
  47. Zhao J, Li N, Warren PV, Golbeck JH, Bryant DA (1992) Sitedirected conversion of a cysteine to aspartate leads to the assembly of a [3Fe.4S] cluster in PsaC of Photosystem I. The photoreduction of FA is independent of FB Biochemistry 31: 5093–5099PubMedCrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • H. T. Witt
    • 1
  1. 1.Max-Volmer-Institut für Biophysikalische und Physikalische ChemieTechnische Universität BerlinBerlinGermany

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