Abstract
In shoots of higher plants, the concentration of many essential elements remains constant in spite of spatio-temporal variation in nutrient availability. This suggests the existence of a homeostasis system that adjusts the uptake to the growth-dependent demand. To identify components of the signalling pathway for Fe homeostasis, a marker-based mutant screen was established. The maker system was constructed from the Fe-deficiency-induced promoter of the AtIRT1 gene and a GUS-reporter gene. A. thalina plants transformed with the construct showed GUS-activity depending on the Fe status of the plant. Under mild Fe deficiency, GUS activity was restricted to the vascular system of older roots. Severe Fe deficiency, however, resulted in high GUS activity in the entire root and weak GUS signals in the shoot vascular system. In a double-screen based on an in-vivo GUS-assay, close to 100 % of plants from a transgenic population were GUS-positive. Based on this assay, a mutant population derived from a transgenic pIRT1-GUS-line by EMS mutagenesis was screened. 32 mutants showed no GUS activity in the screen and decreased Fe reductase activity under hydroponic conditions that induced this enzyme in non-mutagenised pIRT1-GUS plants. The mutants were crossed with wildtype A. thaliana Col-1 plants as pollen donors. The F2 mutant populations will be tested for their ability to induce the Fe deficiency response.
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© 2001 Kluwer Academic Publishers
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Köhl, K.I., Römheld, V., Altmann, T. (2001). Fe sensing in Arabidopsis thaliana. In: Horst, W.J., et al. Plant Nutrition. Developments in Plant and Soil Sciences, vol 92. Springer, Dordrecht. https://doi.org/10.1007/0-306-47624-X_24
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DOI: https://doi.org/10.1007/0-306-47624-X_24
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-7105-2
Online ISBN: 978-0-306-47624-2
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