Roles for Nucleotides in Nitrogenase Catalysis
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In summary, it is clear that nucleotides participate at several levels in the nitrogenase reaction. Binding of nucleotides to the Fe protein result in protein conformational changes that are important for subsequent reaction steps. The hydrolysis of ATP by the Fe protein-MoFe protein complex is not absolutely required for electron transfer from the Fe protein to the MoFe protein, but does greatly accelerate this reaction. Finally, the hydrolysis of ATP to ADP and Pi is involved in controlling the dissociation of the Fe protein from the MoFe protein following electron transfer.
KeywordsElectron Transfer Nucleotide Binding Site Protein Conformational Change MoFe Protein Complex Dissociation
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- Duyvais MG, et al. (1998) Biochemistry 37, 17345–17354.Google Scholar
- Hausinger RP, Howard JB (1983) J. Biol. Chem. 258, 3486–3492.Google Scholar
- Lowe DJ, et al. (1995) in Nitrogen Fixation: Fundamentals and Applications (Tikhonovich IA, et al., Eds.) pp 103–108, Kluwer Academic, Dordrecht.Google Scholar
- Rees DC, et al. (1993) Adv. Inorg. Chem. 40, 89–119.Google Scholar
- Rees DC, et al. (1998) in Biological Nitrogen Fixation for the 21st Century (Elmerich C, et al., Eds.) pp 11–16, Kluwer Academic Publishers, Boston.Google Scholar
- Seefeldt LC, Dean DR (1997) Ace. Chem. Res. 30, 260–266.Google Scholar