Advertisement

Roles for Nucleotides in Nitrogenase Catalysis

  • L. C. Seefeldt
  • J. M. Chan
  • W. Wu
  • J. E. Huyett
Chapter
  • 29 Downloads
Part of the Current Plant Science and Biotechnology in Agriculture book series (PSBA, volume 38)

Summary

In summary, it is clear that nucleotides participate at several levels in the nitrogenase reaction. Binding of nucleotides to the Fe protein result in protein conformational changes that are important for subsequent reaction steps. The hydrolysis of ATP by the Fe protein-MoFe protein complex is not absolutely required for electron transfer from the Fe protein to the MoFe protein, but does greatly accelerate this reaction. Finally, the hydrolysis of ATP to ADP and Pi is involved in controlling the dissociation of the Fe protein from the MoFe protein following electron transfer.

Keywords

Electron Transfer Nucleotide Binding Site Protein Conformational Change MoFe Protein Complex Dissociation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Burgess BK, Lowe DJ (1996) Chem. Rev. 96, 2983–3011.PubMedCrossRefGoogle Scholar
  2. Chan JM, et al. (1999) J. Biol. Chem. 274, 17593–17598.PubMedGoogle Scholar
  3. Chan MK, et al. (1993) Science 260, 792–794.PubMedGoogle Scholar
  4. Dean DR, et al. (1993) J. Bacteriol. 175, 6737–6744.PubMedGoogle Scholar
  5. Duyvais MG, et al. (1998) Biochemistry 37, 17345–17354.Google Scholar
  6. Georgiadis MM, et al. (1992) Science 257, 1653–1659.PubMedGoogle Scholar
  7. Hageman RV, Burns RH (1978) Proc. Natl. Acad. Sci. U.S.A. 75, 2699–2702.PubMedGoogle Scholar
  8. Hagen WR, et al. (1987) Eur. J. Biochem. 169, 457–465.PubMedCrossRefGoogle Scholar
  9. Hausinger RP, Howard JB (1983) J. Biol. Chem. 258, 3486–3492.Google Scholar
  10. Howard JB, et al. (1989) J. Biol. Chem. 264, 11270–11274.PubMedGoogle Scholar
  11. Lanzilotta WN, et al. (1996) Biochemistry 35, 7188–7196.PubMedGoogle Scholar
  12. Lanzilotta WN, et al. (1997) J. Biol. Chem. 272, 4157–4165.PubMedGoogle Scholar
  13. Lanzilotta WN, Seefeldt LC (1997) Biochemistry 36, 12976–12983.PubMedCrossRefGoogle Scholar
  14. Larsen C, et al. (1995) Arch. Biochem. Biophys. 323, 215–222.PubMedCrossRefGoogle Scholar
  15. Lowe DJ, et al. (1995) in Nitrogen Fixation: Fundamentals and Applications (Tikhonovich IA, et al., Eds.) pp 103–108, Kluwer Academic, Dordrecht.Google Scholar
  16. Peters JW, et al. (1994) J. Biol. Chem. 269, 28076–28083.PubMedGoogle Scholar
  17. Rees DC, et al. (1993) Adv. Inorg. Chem. 40, 89–119.Google Scholar
  18. Rees DC, et al. (1998) in Biological Nitrogen Fixation for the 21st Century (Elmerich C, et al., Eds.) pp 11–16, Kluwer Academic Publishers, Boston.Google Scholar
  19. Renner KA, Howard JB (1996) Biochemistry 35, 5353–5358.PubMedCrossRefGoogle Scholar
  20. Ryle MJ, Seefeldt LC (1996) Biochemistry 35, 4766–4775.PubMedGoogle Scholar
  21. Schindelin H, et al. (1997) Nature 387, 370–376.PubMedCrossRefGoogle Scholar
  22. Schlessman JL, et al. (1998) J. Mol. Biol. 280, 669–685.PubMedCrossRefGoogle Scholar
  23. Seefeldt LC, Dean DR (1997) Ace. Chem. Res. 30, 260–266.Google Scholar
  24. Seefeldt LC, et al. (1992) J. Biol. Chem. 267, 6680–6688.PubMedGoogle Scholar
  25. Seefeldt LC, Mortenson LE (1993) Protein Sci. 2, 93–102.PubMedGoogle Scholar
  26. Shah VK, Brill WJ (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 3249–3253.PubMedGoogle Scholar
  27. Thorneley RNF, Lowe DJ (1983) Biochem. J. 215, 393–403.PubMedGoogle Scholar
  28. Thorneley RNF, Lowe DJ (1984) Biochem. J. 224, 887–894.PubMedGoogle Scholar
  29. Watt GD, et al. (1980) Biochemistry 19, 4926–4932.PubMedGoogle Scholar
  30. Wolle D, et al. (1992) Science 258, 992–995.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • L. C. Seefeldt
    • 1
  • J. M. Chan
    • 1
  • W. Wu
    • 1
  • J. E. Huyett
    • 1
  1. 1.Department of Chemistry and BiochemistryUtah State UniversityLoganUSA

Personalised recommendations