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The Cell Membrane and Transport

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Abstract

Membranes define cellular units. Membranes are integral parts in the basic concept of the cell as the smallest unit of life. This is structurally evident for the smallest and simplest of cells, i.e. Mollicutes and certain Buchnera symbionts, as well as for the largest and most elaborate eukaryotic cells with many membrane-surrounded compartments and organelles housing various specialized functions and systems. Analyses of the potential numbers of encoded membrane proteins in a number of various, sequenced genomes substantiates the importance of membrane proteins, indicating that proteins with one, or more, transmembrane segments constitute 20 to 30% of all cellular protein species97, 108. Furthermore, certain membrane transporters and receptors constitute the major protein classes in prokaryotes and eukaryotes.

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References

  1. Andersson, A. S., L. Rilfors, R. N. Lewis, R. N. McElhaney, and G. Lindblom. 1998. Occurrence of monoacyl-diglucosyl-diacyl-glycerol and monoacyl-bis-glycerophosphoryl-diglucosyl-diacyl-glycerol in membranes of Acholeplasma laidlawii strain B-PG9. Biochim. Biophys. Acta 1389:43–49.

    PubMed  CAS  Google Scholar 

  2. Andersson, A. S., L. Rilfors, G. Orädd, and G. Lindblom. 1998. Total lipids with short and long acyl chains from Acholeplasma laidlawii form nonlamellar phases. Biophys. J. 75:2877–2887.

    PubMed Central  PubMed  CAS  Google Scholar 

  3. Antelmann, H., H. Tjalsma, B. Voigt, S. Ohlmeier, S. Bron, J. M. van Dijl, and M. Hecker. 2001. A proteomic view on genome-based signal peptide predictions. Genome Research 11:1484–502.

    PubMed  CAS  Google Scholar 

  4. Barile, M. F., and S. Razin. 1979. The Mycoplasmas, vol. 1. Academic Press, New York.

    Google Scholar 

  5. Ben-Menachem, G., T. Byström, H. Rechnitzer, S. Rottem, L. Rilfors, and G. Lindblom. 2001. The physico-chemical characteristics of the phosphocholine-containing glycoglycerolipid MfGL-II govern the permeability properties of Mycoplasma fermentans. Eur. J. Biochem. 268:3694–3701.

    PubMed  CAS  Google Scholar 

  6. Ben-Menachem, G., F. Wagner, U. Zähringer, E. T. Rietschel, and S. Rottem. 1997. Antibody response to MfGL-II, a phosphocholine-containing major lipid of Mycoplasma fermentans membranes. FEMS Microbiol. Lett. 154:363–369.

    PubMed  CAS  Google Scholar 

  7. Berg, S., M. Edman, L. Li, M. Vikström, and A. Wieslander. 2001. Sequence properties of the 1,2-diacylglycerol 3-glucosyltransferase from Acholeplasma laidlawii membranes. Recognition of a large group of lipid glycosyltransferases in eubacteria and archaea. J. Biol. Chem. 276:22056–22063.

    PubMed  CAS  Google Scholar 

  8. Berg, S., and Å. Wieslander. 1997. Purification of a phosphatase which hydrolyzes phosphatidic acid, a key intermediate in glucolipid synthesis in Acholeplasma laidlawii A membranes. Biochim. Biophys. Acta 1330:225–232.

    PubMed  CAS  Google Scholar 

  9. Beven, L., and H. Wrobléwski. 1997. Effect of natural amphipathic peptides on viability, membrane potential, cell shape and motility of mollicutes. Res. Microbiol. 148:163–175.

    PubMed  CAS  Google Scholar 

  10. Blattner, F. R., G. Plunkett, C. A. Bloch, N. T. Perna, V. Burland, M. Riley, J. Collado-Vides, J. D. Glasner, C. K. Rode, G. F. Mayhew, J. Gregor, N. W. Davis, H. A. Kirkpatrick, M. A. Goeden, D. J. Rose, B. Mau, and Y. Shao. 1997. The complete genome sequence of Escherichia coli K-12. Science 277:1453–1474.

    PubMed  CAS  Google Scholar 

  11. Campbell, J. A., G. J. Davies, V. Bulone, and B. Henrissat. 1997. A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities. Biochem. J. 326:929–939.

    PubMed Central  PubMed  CAS  Google Scholar 

  12. Cartee, R. T., W. T. Forsee, J. W. Jensen, and J. Yother. 2001. Expression of the Streptococcus pneumoniae type 3 synthase in Escherichia coli. Assembly of type 3 polysaccharide on a lipid primer. J. Biol. Chem. 276:48831–48839.

    PubMed  CAS  Google Scholar 

  13. Chambaud, I., R. Heilig, S. Ferris, V. Barbe, D. Samson, F. Galisson, I. Moszer, K. Dybvig, H. Wrobléwski, A. Viari, E. P. Rocha, and A. Blanchard. 2001. The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis. Nucleic Acids Res. 29:2145–2153.

    PubMed Central  PubMed  CAS  Google Scholar 

  14. Chambaud, L, H. Wrobléwski, and A. Blanchard. 1999. Interactions between mycoplasma lipoproteins and the host immune system. Trends Microbiol. 7:493–499.

    PubMed  CAS  Google Scholar 

  15. Christiansson, A., L. E. Eriksson, J. Westman, R. Demel, and Â. Wieslander. 1985. Involvement of surface potential in regulation of polar membrane lipids in Acholeplasma laidlawii. J. Biol. Chem. 260:3984–3990.

    PubMed  CAS  Google Scholar 

  16. Cirillo, V. P. 1993. Transport systems in mycoplasmas. Subcellular Biochemistry 20:293–310.

    PubMed  CAS  Google Scholar 

  17. Clementz, T., A. Christiansson, and Å. Wieslander. 1986. Transmembrane electrical potential affects the lipid composition of Acholeplasma laidlawii. Biochemistry. 25:823–830.

    PubMed  CAS  Google Scholar 

  18. Cullen, J., M. C. Phillips, and G. G. Shipley. 1971. The effects of temperature on the composition and physical properties of the lipids of Pseudomonas fluorescens. Biochemical J. 125:733–742.

    CAS  Google Scholar 

  19. Dahl, J. 1993. The role of cholesterol in mycoplasma membranes. Subcellular Biochemistry 20:167–188.

    PubMed  CAS  Google Scholar 

  20. Dahlqvist, A., S. Nordström, O. P. Karlsson, D. A. Mannock, R. N. McElhaney, and Å. Wieslander. 1995. Efficient modulation of glucolipid enzyme activities in membranes of Acholeplasma laidlawii by the type of lipids in the bilayer matrix. Biochemistry. 34:13381–13389.

    PubMed  CAS  Google Scholar 

  21. Dandekar, T., M. Huynen, J. T. Regula, B. Ueberle, C. U. Zimmermann, M. A. Andrade, T. Doerks, L. Sanchez-Pulido, B. Snel, M. Suyama, Y. P. Yuan, R. Herrmann, and P. Bork. 2000. Re-annotating the Mycoplasma pneumoniae genome sequence: adding value, function and reading frames. Nucleic. Acids. Res. 28:3278–3288.

    PubMed Central  PubMed  CAS  Google Scholar 

  22. Dañino, D., A. Kaplun, G. Lindblom, L. Rilfors, G. Orädd, J. B. Hauksson, and Y. Talmon. 1997. Cryo-TEM and NMR studies of a micelle-forming phosphoglucolipid from membranes of Acholeplasma laidlawii A and B. Chem. Phys. Lipids. 85:75–89.

    PubMed  Google Scholar 

  23. de Gier, J. W., and J. Luirink. 2001. Biogenesis of inner membrane proteins in Escherichia coli. Mol. Microbiol. 40:314–322.

    PubMed  Google Scholar 

  24. Edman, M., S. Berg, P. Storm, M. Wikström, S. Vikström, A. Öhman, and Å. Wieslander. 2002. Similar structure features of two glycosyltransferases synthesizing the major non-bilayer and bilayer glycolipids lipids in Acholeplasma laidlawii. Submitted for publication.

    Google Scholar 

  25. Edman, M., T. Jarhede, M. Sjöström, and Å. Wieslander. 1999. Different sequence patterns in signal peptides from mycoplasmas, other gram-positive bacteria, and Escherichia coli: a multivariate data analysis. Proteins 35:195–205.

    PubMed  CAS  Google Scholar 

  26. Edman, M., M. Sjöström, and A. Wieslander. 2002. Multivariate analysis of ABC-dependent membrane transport proteins from five genomes reveals group-specific features in sequence properties. Under revision.

    Google Scholar 

  27. Efrati, H., Y. Wax, and S. Rottem. 1986. Cholesterol uptake capacity of Acholeplasma laidlawii is affected by the composition and content of membrane glycolipids. Arch. Biochem. Biophys. 248:282–288.

    PubMed  CAS  Google Scholar 

  28. Epand, R. M., and R. F. Epand. 1994. Calorimetric detection of curvature strain in phospholipid bilayers. Biophys. J. 66:1450–1456.

    PubMed Central  PubMed  CAS  Google Scholar 

  29. Eriksson, P. O., L. Rilfors, Å. Wieslander, A. Lundberg, and G. Lindblom. 1991. Order and dynamics in mixtures of membrane glucolipids from Acholeplasma laidlawii studied by 2H NMR. Biochemistry 30:4916–4924.

    PubMed  CAS  Google Scholar 

  30. Fischetti, V. A., R. P. Novick, J. J. Ferretti, D. A. Portnoy, and J. I. Rood. 2000. Gram-Positive Pathogens. ASM Press, Washington D.C.

    Google Scholar 

  31. Foht, P. J, Q. M. Tran, R. N. Lewis, and R. N. McElhaney. 1995. Quantitation of the phase preferences of the major lipids of the Acholeplasma laidlawii B membrane. Biochemistry 34:13811–13817.

    PubMed  CAS  Google Scholar 

  32. Glass, J. I., E. J. Lefkowitz, J. S. Glass, C. R. Heiner, E. Y. Chen, and G. H. Cassell. 2000. The complete sequence of the mucosal pathogen Ureaplasma urealyticum. Nature 407:757–762.

    PubMed  CAS  Google Scholar 

  33. Goerke, J. 1998. Pulmonary surfactant: functions and molecular composition. Biochim. Biophys. Acta 1408:79–89.

    PubMed  CAS  Google Scholar 

  34. Goldfine, H. 1993. Phospholipid biosynthetic enzymes of butyric acid-producing Clostridia. In Sebald, M. (ed.), Genetics and Molecular Biology of Anaerobes, Springer Verlag, New York.

    Google Scholar 

  35. Grüner, S. M. 1989. Stability of lyotropic phase with curved interfaces. J. Phys. Chem. 93:7562–7570.

    Google Scholar 

  36. Hacker, J., and E. Carniel. 2001. Ecological fitness, genomic islands and bacterial pathogenicity. A Darwinian view of the evolution of microbes. EMBO reports 2:376–381.

    PubMed Central  PubMed  CAS  Google Scholar 

  37. Hase, C. C, N. D. Fedorova, M. Y. Galperin, and P. A. Dibrov. 2001. Sodium ion cycle in bacterial pathogens: evidence from cross-genome comparisons. Microbiol. Mol. Biol. Rev. 65:353–370.

    PubMed Central  PubMed  CAS  Google Scholar 

  38. Hauksson, J. B., G. Lindblom, and L. Rilfors. 1994. Structures of glucolipids from the membrane of Acholeplasma laidlawii strain A-EF22. II. Monoacylmonoglucosyldiacylglycerol. Biochim. Biophys. Acta. 1215:341–345.

    PubMed  Google Scholar 

  39. Hauksson, J. B., L. Rilfors, G. Lindblom, and G. Arvidson. 1995. Structures of glucolipids from the membrane of Acholeplasma laidlawii strain A-EF22. III. Monoglucosyldiacylglycerol, diglucosyldiacylglycerol, and monoacyldiglucosyldiacylglycerol. Biochim. Biophys. Acta 1258:1–9.

    PubMed  Google Scholar 

  40. Herrmann, R., and B. Reiner. 1998. Mycoplasma pneumoniae and Mycoplasma genitalium: a comparison of two closely related bacterial species. Curr. Opin. Microbiol. 1:572–579.

    PubMed  CAS  Google Scholar 

  41. Himmelreich, R., H. Hilbert, H. Plagens, E. Pirkl, B. C. Li, and R. Herrmann. 1996. Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae. Nucleic. Acids Res. 24:4420–4449.

    PubMed Central  PubMed  CAS  Google Scholar 

  42. Hutchison, C. A., S. N. Peterson, S. R. Gill, R. T. Cline, O. White, C. M. Fraser, H. O. Smith, and J. C. Venter. 1999. Global transposon mutagenesis and a minimal Mycoplasma genome. Science 286:2165–2169.

    PubMed  CAS  Google Scholar 

  43. Israelachvili, J. N., D. J. Mitchell, and B. W. Ninham. 1976. Theory of self-assembly of hydrocarbon amphiphiles into micelles and bilayers. J. Chem. Soc. Faraday Trans. II. 72:1525–1568.

    Google Scholar 

  44. Jayasinghe, S., K. Hristova, and S. H. White. 2001. Energetics, stability, and prediction of transmembrane helices. J. Mol. Biol. 312:927–934.

    PubMed  CAS  Google Scholar 

  45. Jongbloed, J. D., U. Martin, H. Antelmann, M. Hecker, H. Tjalsma, G. Venema, S. Bron, J. M. van Dijl, and J. Muller. 2000. TatC is a specificity determinant for protein secretion via the twin-arginine translocation pathway. J. Biol. Chem. 275:41350–41357.

    PubMed  CAS  Google Scholar 

  46. Karlsson, O. P., A. Dahlqvist, S. Vikström, and Å. Wieslander. 1997. Lipid dependence and basic kinetics of the purified 1,2-diacylglycerol 3-glucosyltransferase from membranes of Acholeplasma laidlawii. J. Biol. Chem. 272:929–936.

    PubMed  CAS  Google Scholar 

  47. Karlsson, O. P., M. Rytömaa, A. Dahlqvist, P. K. Kinnunen, and Å. Wieslander. 1996. Correlation between bilayer lipid dynamics and activity of the diglucosyldiacylglycerol synthase from Acholeplasma laidlawii membranes. Biochemistry 35:10094–10102.

    PubMed  CAS  Google Scholar 

  48. Koonin, E. V. 2000. Bridging the gap between sequence and function. Trends Genetics 16:16.

    CAS  Google Scholar 

  49. Krause, D. C, and M. F. Balish. 2001. Structure, function, and assembly of the terminal organelle of Mycoplasma pneumoniae. FEMS Microbiol. Lett. 198:1–7.

    PubMed  CAS  Google Scholar 

  50. Kunst, F., N. Ogasawara, I. Moszer, A. M. Albertini, G. Alloni, V. Azevedo, M. G. Bertero, P. Bessieres, A. Bolotin, S. Borchert, R. Borriss, L. Boursier, A. Brans, M. Braun, S. C. Brignell, S. Bron, S. Brouillet, C. V. Bruschi, B. Caldwell, V. Capuano, N. M. Carter, S. K. Choi, J. J. Codani, I. F. Connerton, and A. Danchin. 1997. The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature 390:249–256.

    PubMed  CAS  Google Scholar 

  51. Le Henaff, M., A. Chollet, and C. Fontenelle. 2001. Chemical analysis of lipid-modified membrane proteins in Acholeplasma laidlawii. Curr. Microbiol. 43:424–428.

    PubMed  CAS  Google Scholar 

  52. Lewis, R. N., and R. N. McElhaney. 1995. Acholeplasma laidlawii B membranes contain a lipid (glycerylphosphoryldiglucosyldiacylglycerol) which forms micelles rather than lamellar or reversed phases when dispersed in water. Biochemistry 34:13818–13824.

    PubMed  CAS  Google Scholar 

  53. Lewis, R. N., A. W. Yue, R. N. McElhaney, D. C. Turner, and S. M. Grüner. 1990. Thermotropic characterization of the 2–0-acyl,polyprenyl alpha-D-glucopyranoside isolated from palmitate-enriched Acholeplasma laidlawii B membranes. Biochim. Biophys. Acta 1026:21–28.

    PubMed  CAS  Google Scholar 

  54. Li, L., O. P. Karlsson, P. Storm, S. Berg, and A. Wieslander. 2002. Binding governs activity of the 1,2-diacylglycerol 3-glucosyltransferase from Acholeplasma laidlawii at the lipid bilayer surface. To be submitted.

    Google Scholar 

  55. Lindblom, G., I. Brentel, M. Sjölund, G. Wikander, and Å. Wieslander. 1986. Phase equilibria of membrane lipids from Acholeplasma laidlawii: importance of a single lipid forming nonlamellar phases. Biochemistry 25:7502–7510.

    PubMed  CAS  Google Scholar 

  56. Lindblom, G., J. B. Hauksson, L. Rilfors, B. Bergenståhl, Å. Wieslander, and P. O. Eriksson. 1993. Membrane lipid regulation in Acholeplasma laidlawii grown with saturated fatty acids. Biosynthesis of a triacylglucolipid forming reversed micelles. J. Biol. Chem. 268:16198–16207.

    PubMed  CAS  Google Scholar 

  57. Maniloff, J., R. N. McElhaney, L. R. Finch, and J. B. Baseman (eds.). 1992. Mycoplasmas, molecular biology and pathogenesis. ASM Press, Washington D.C.

    Google Scholar 

  58. Mannock, D. A., P. E. Harper, S. M. Grüner, and R. N. McElhaney. 2001. The physical properties of glycosyl diacylglycerols. Calorimetric, X-ray diffraction and Fourier transform spectroscopic studies of a homologous series of l,2-di-O-acyl-3-O-(beta-D-galactopyranosyl)-sn-glycerols. Chem. Phys. Lipids 111:139–161.

    PubMed  CAS  Google Scholar 

  59. Marr, A. G., and J. L. Ingraham. 1962. Effect of temperature on the composition of fatty acids in Escherichia coll J. Bacteriol. 84:1260–1267.

    PubMed Central  PubMed  CAS  Google Scholar 

  60. Mazmanian, S. K., G. Liu, H. Ton-That, and O. Schneewind. 1999. Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall. Science 285:760–763.

    PubMed  CAS  Google Scholar 

  61. McElhaney. 1992. Membrane structure, p. 113–157. In J. Maniloff, R. N. McElhaney, L. R. Finch, and J. B. Baseman (ed.), Mycoplasmas, Molecular biology and pathogenesis. ASM Press, Washington D.C.

    Google Scholar 

  62. McElhaney. 1992. Lipid incorporation, biosynthesis, and metabolism, p. 231–259. In J. Maniloff, R. N. McElhaney, L. R. Finch, and J. B. Baseman (ed.), Mycoplasmas, Molecular biology and pathogenesis. ASM Press, Washington D.C.

    Google Scholar 

  63. McMullen, T. P., B. C. Wong, E. L. Tham, R. N. Lewis, and R. N. McElhaney. 1996. Differential scanning calorimetric study of the interaction of cholesterol with the major lipids ofthe Acholeplasma laidlawii B membrane. Biochemistry 35:16789–16798.

    PubMed  CAS  Google Scholar 

  64. Minion, F. C, and R. F. Rosenbusch. 1993. Extramembranous structure in mycoplasmas. Subcellular Biochemistry 20:189–201.

    PubMed  CAS  Google Scholar 

  65. Morein, S., A. Andersson, L. Rilfors, and G. Lindblom. 1996. Wild-type Escherichia coli cells regulate the membrane lipid composition in a “window” between gel and non-lamellar structures. J. Biol. Chem. 271:6801–6809.

    PubMed  CAS  Google Scholar 

  66. Navarre, W. W., and O. Schneewind. 1999. Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope. Microbiol. Mol. Biol. Rev. 63:174–229;

    PubMed Central  PubMed  CAS  Google Scholar 

  67. Nyström, S., P. Wallbrandt, and Ä. Wieslander. 1992. Membrane protein acylation. Preference for exogenous myristic acid or endogenous saturated chains in Acholeplasma laidlawii. Eur. J. Biochem. 204:231–240.

    PubMed  Google Scholar 

  68. Österberg, F., L. Rilfors, Å. Wieslander, G. Lindblom, and S. M. Gruner. 1995. Lipid extracts from membranes of Acholeplasma laidlawii A grown with different fatty acids have a nearly constant spontaneous curvature. Biochim. Biophys. Acta 1257:18–24.

    PubMed  Google Scholar 

  69. Paulsen, I. T., L. Nguyen, M. K. Sliwinski, R. Rabus, and M. H. Saier. 2000. Microbial genome analyses: comparative transport capabilities in eighteen prokaryotes. J. Mol. Biol. 301:75–100.

    PubMed  CAS  Google Scholar 

  70. Paulsen, I. T., M. K. Sliwinski, and M. H. Saier. 1998. Microbial genome analyses: global comparisons of transport capabilities based on phylogenies, bioenergetics and substrate specificities J. Mol. Biol. 277:573–592.

    PubMed  CAS  Google Scholar 

  71. Peterson, S. N., and C. M. Fraser. 2001. The complexity of simplicity. Genome Biology 2: COMMENT2002

    Google Scholar 

  72. Plackett, P., B. P. Marmion, E. J. Shaw, and R. M. Lemcke. 1969. Immunochemical analysis of Mycoplasma pneumoniae. 3. Separation and chemical identification of serologically active lipids. Aust. J .Exp. Biol. Med. Sci. 47:171–195.

    PubMed  CAS  Google Scholar 

  73. Plano, G. V., J. B. Day, and F. Ferracci. 2001. Type III export: new uses for an old pathway. Mol. Microbiol. 40:284–293.

    PubMed  CAS  Google Scholar 

  74. Pollack, J. D. 1997. Mycoplasma genes: a case for reflective annotation. Trends Microbiol. 5:413–419.

    PubMed  CAS  Google Scholar 

  75. Pollack, J. D., M. V. Williams, and R. N. McElhaney. 1997. The comparative metabolism of the mollicutes (Mycoplasmas): the utility for taxonomic classification and the relationship of putative gene annotation and phylogeny to enzymatic function in the smallest free-living cells. Crit. Rev. Microbiol. 23:269–354.

    PubMed  CAS  Google Scholar 

  76. Prenner, E. J., R. N. Lewis, K. C. Neuman, S. M. Gruner, L. H. Kondejewski, R. S. Hodges, and R. N. McElhaney. 1997. Nonlamellar phases induced by the interaction of gramicidin S with lipid bilayers. A possible relationship to membrane-disrupting activity. Biochemistry 36:7906–7916.

    PubMed  CAS  Google Scholar 

  77. Pyrowolakis, G., D. Hofmann, and R. Herrmann. 1998. The subunit b of the F0F1-type ATPase of the bacterium Mycoplasma pneumoniae is a lipoprotein. J. Biol. Chem. 273:24792–24796.

    PubMed  CAS  Google Scholar 

  78. Razin, S., D. Yogev, and Y. Naot. 1998. Molecular biology and pathogenicity of mycoplasmas. Microbiol. Mol. Biol. Rev. 62:1094–1156.

    PubMed Central  PubMed  CAS  Google Scholar 

  79. Regula, J. T., B. Ueberle, G. Boguth, A. Gorg, M. Schnolzer, R. Herrmann, and R. Frank. 2000. Towards a two-dimensional proteome map of Mycoplasma pneumoniae. Electrophoresis 21:3765–3780.

    PubMed  CAS  Google Scholar 

  80. Rietveld, A. G., J. A. Killian, W. Dowhan, and B. de Kruijff. 1993. Polymorphic regulation of membrane phospholipid composition in Escherichia coli. J. Biol Chem. 268:12427–12433.

    PubMed  CAS  Google Scholar 

  81. Rilfors, L., G. Lindblom, Å. Wieslander, and A. Christiansson. 1984. Lipid bilayer stability in biological membranes, p. 205–245. In M. Kates, and M. L.A. (ed.), Membrane fluidity. Plenum Press, New York.

    Google Scholar 

  82. Rilfors, L., G. Wikander, and Å. Wieslander. 1987. Lipid acyl chain-dependent effects of sterols in Acholeplasma laidlawii membranes. J. Bacteriol. 169:830–838.

    PubMed Central  PubMed  CAS  Google Scholar 

  83. Rosén, M., and Å. Wieslander. 2002. Extensive membrane glycolipid synthesis in the small Mycoplasma pneumoniae: Recognition of three glycosyltransferases. Under revision.

    Google Scholar 

  84. Rottem, S., L. Adar, Z. Gross, Z. Ne’eman, and P. J. Davis. 1986. Incorporation and modification of exogenous phosphatidylcholines by mycoplasmas. J. Bacteriol. 167:299–304.

    PubMed Central  PubMed  CAS  Google Scholar 

  85. Rottem, S., and I. Kahane (eds.). 1993. Mycoplasma Cell Membranes. Subcellular Biochemistry vol. 20.

    Google Scholar 

  86. Saier, M. H. 2000. A functional-phylogenetic classification system for transmembrane solute transporters. Microbiol. Mol. Biol. Rev. 64:354–411.

    PubMed Central  PubMed  CAS  Google Scholar 

  87. Saleh, M. T., M. Fillon, P. J. Brennan, and J. T. Belisle. 2001. Identification of putative exported/secreted proteins in prokaryotic proteomes. Gene 269:195–204.

    PubMed  CAS  Google Scholar 

  88. Samuelson, J. C., M. Chen, F. Jiang, I. Moller, M. Wiedmann, A. Kuhn, G. J. Phillips, and R. E. Dalbey. 2000. YidC mediates membrane protein insertion in bacteria. Nature 406:637–641.

    PubMed  CAS  Google Scholar 

  89. Schwartz, R., C. S. Ting, and J. King. 2001. Whole proteome pI values correlate with subcellular localizations of proteins for organisms within the three domains of life. Genome Research 11:703–709.

    PubMed  CAS  Google Scholar 

  90. Shigenobu, S., H. Watanabe, M. Hattori, Y. Sakaki, and H. Ishikawa. 2000. Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS. Nature 407:81–86.

    PubMed  CAS  Google Scholar 

  91. Shirvan, M. H., and S. Rottem. 1993. Ion pumps and volume regulation in mycoplasma. Subcellular Biochemistry 20:261–292.

    PubMed  CAS  Google Scholar 

  92. Sinensky, M. 1974. Homeoviscous adaptation—A homeostatic process that regulates the viscosity of membrane lipids in Escherichia coli. Proc. Nat. Acad. Sci. USA 71:522–525.

    PubMed Central  PubMed  CAS  Google Scholar 

  93. Singer, S. J., and G. L. Nicolson. 1972. The fluid mosaic model of the structure of cell membranes. Science 175:720–731.

    PubMed  CAS  Google Scholar 

  94. Sjostrom, M., S. Rännar, and Å. Wieslander. 1995. Polpeptide sequence property relationships in Escherichia coli based on auto cross covariances. Chemom. Intell. Lab. Syst. 29:295–305.

    Google Scholar 

  95. Smith, P. F. 1992. Membrane lipid and lipopolysaccharide structures, p. 79–93. In J. Maniloff, R. N. McElhaney, L. R. Finch, and J. B. Baseman (ed.), Mycoplasmas, Molecular biology and pathogenesis. ASM Press, Washington D.C.

    Google Scholar 

  96. Staudegger, E., E. J. Prenner, M. Kriechbaum, G. Degovics, R. N. Lewis, R. N. McElhaney, and K. Lohner. 2000. X-ray studies on the interaction of the antimicrobial peptide gramicidin S with microbial lipid extracts: evidence for cubic phase formation. Biochim. Biophys. Acta 1468:213–230.

    PubMed  CAS  Google Scholar 

  97. Stevens, T. J., and I. T. Arkin. 2000. Do more complex organisms have a greater proportion of membrane proteins in their genomes? Proteins 39:417–420.

    PubMed  CAS  Google Scholar 

  98. Teichmann, S. A., C. Chothia, and M. Gerstein. 1999. Advances in structural genomics. Curr. Opin. Struct. Biol. 9:390–399.

    PubMed  CAS  Google Scholar 

  99. Terada, M., T. M. Kuroda, Si S., and H. Tokuda. 2001. Lipoprotein sorting signals evaluated as the LolA-dependent release of lipoproteins from the cytoplasmic membrane of Escherichia coli. J. Biol. Chem. 276:47690–47694.

    PubMed  CAS  Google Scholar 

  100. Thurmond, R. L., A. R. Niemi, G. Lindblom, Å. Wieslander, and L. Rilfors. 1994. Membrane thickness and molecular ordering in Acholeplasma laidlawii strain A studied by 2H NMR spectroscopy. Biochemistry 33:13178–13188.

    PubMed  CAS  Google Scholar 

  101. Tilcock, C. P., M. J. Hope and P. R. Cullis. 1984. Influence of cholesterol esters of varying unsaturation on the polymorphic phase preference of egg phosphatidylethanolamine. Chem. Phys. Lipids 35: 363–370.

    CAS  Google Scholar 

  102. Tjalsma, H., A. Bolhuis, J. D. Jongbloed, S. Bron, and J. M. van Dijl. 2000. Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretóme. Microbiol. Mol. Biol. Rev. 64:515–547.

    PubMed Central  PubMed  CAS  Google Scholar 

  103. van Wely, K. H., J. Swaving, R. Freudl, and A. J. Driessen. 2001. Translocation of proteins across the cell envelope of Gram-positive bacteria. FEMS Microbiol. Rev. 25:437–454.

    PubMed  Google Scholar 

  104. Veldhuizen, R., K. Nag, S. Orgeig, and F. Possmayer. 1998. The role of lipids in pulmonary surfactant. Biochim. Biophys. Acta 1408:90–108.

    PubMed  CAS  Google Scholar 

  105. Vikström, S., L. Li, O. P. Karlsson, and Å. Wieslander. 1999. Key role of the diglucosyldiacylglycerol synthase for the nonbilayer-bilayer lipid balance of Acholeplasma laidlawii membranes. Biochemistry 38:5511–5520.

    PubMed  Google Scholar 

  106. Vikström, S., L. Li, and A. Wieslander. 2000. The nonbilayer/bilayer lipid balance in membranes. Regulatory enzyme in Acholeplasma laidlawii is stimulated by metabolic phosphates, activator phospholipids, and double-stranded DNA. J Biol Chem. 275: 9296–9302.

    PubMed  Google Scholar 

  107. von Heijne, G. 1992. Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule. J. Mol. Biol. 225:487–494.

    Google Scholar 

  108. Wallin, E., and G. von Heijne. 1998. Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms. Protein Sci. 7:1029–1038.

    PubMed Central  PubMed  CAS  Google Scholar 

  109. Wieslander, Å., M. J. Boyer, and Wróblewski. 1992. Membrane protein structure, p. 93–113. In J. Maniloff, R. N. McElhaney, L. R. Finch, and J. B. Baseman (ed.), Mycoplasmas, Molecular biology and pathogenesis. ASM Press, Washington D.C.

    Google Scholar 

  110. Wieslander, Å., A. Christiansson, L. Rilfors, and G. Lindblom. 1980. Lipid bilayer stability in membranes. Regulation of lipid composition in Acholeplasma laidlawii as governed by molecular shape. Biochemistry 19:3650–3655.

    PubMed  CAS  Google Scholar 

  111. Wieslander, Å., S. Nordström, A. Dahlqvist, L. Rilfors, and G. Lindblom. 1995. Membrane lipid composition and cell size of Acholeplasma laidlawii strain A are strongly influenced by lipid acyl chain length. Eur. J Biochem. 227:734–744.

    PubMed  CAS  Google Scholar 

  112. Wieslander, Å., L. Rilfors, and G. Lindblom. 1986. Metabolic changes of membrane lipid composition in Acholeplasma laidlawii by hydrocarbons, alcohols, and detergents: arguments for effects on lipid packing. Biochemistry 25:7511–7517.

    PubMed  CAS  Google Scholar 

  113. Yakushi, T., K. Masuda, S. Narita, S. Matsuyama, and H. Tokuda. 2000. A new ABC transporter mediating the detachment of lipid-modified proteins from membranes. Nature Cell Biol. 2:212–218.

    PubMed  CAS  Google Scholar 

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Wieslander, Å., Rosén, M. (2002). The Cell Membrane and Transport. In: Razin, S., Herrmann, R. (eds) Molecular Biology and Pathogenicity of Mycoplasmas. Springer, Boston, MA. https://doi.org/10.1007/0-306-47606-1_7

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