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Cytadherence and the Cytoskeleton

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Molecular Biology and Pathogenicity of Mycoplasmas

Abstract

In order to associate specifically with host cells, Mycoplasma pneumoniae and closely related species employ a specialized polar structure, the attachment organelle, which is assembled from a set of unique proteins (49–51). Many of these cytadherence-associated proteins exhibit insolubility in the nonionic detergent Triton X-100 (TX) and are associated with a structure, the triton shell, that remains after extraction of cells with TX. Because of its solubility properties, its appearance, and its association with various features of cell morphology, cell motility, cell division, and cell-cell adhesion, this triton shell is regarded as a novel bacterial cytoskeleton.

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References

  1. Akiyama, Y., T. Yoshihisa, K. Ito. 1995. FtsH, a membrane-bound ATPase, forms a complex in the cytoplasmic membrane of Escherichia coli. J. Biol Chem. 270:23485–23490.

    Article  PubMed  CAS  Google Scholar 

  2. Arner, E. S., and A. Holmgren. 2000. Physiological functions of thioredoxin and thioredoxin reductase. Eur. J. Biochem. 267:6102–6109.

    Article  PubMed  CAS  Google Scholar 

  3. Balish, M. F., T.-W. Hahn, P. L. Popham, and D. C. Krause. 2001. Stability of Mycoplasma pneumoniae cytadherence protein HMW1 correlates with its association with the triton shell. J. Bacteriol. 183:3680–3688.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  4. Baseman, J. B., R. M. Cole, D. C. Krause, and D. K. Leith. 1982. Molecular basis for cytadsorption of Mycoplasma pneumoniae. J. Bacteriol. 151:1514–1522.

    PubMed Central  PubMed  CAS  Google Scholar 

  5. Baseman, J. B., J. Morrison-Plummer, D. Drouillard, B. Puleo-Scheppke, V. V. Tryon, and S. C. Holt. 1987. Identification of a 32-kilodalton protein of Mycoplasma pneumoniae associated with hemadsorption. Isr. J. Med. Sci. 23:474–479.

    PubMed  CAS  Google Scholar 

  6. Boatman, E. S. 1979. Morphology and ultrastructure of the mycoplasmatales, p. 63–102. In M. F. Barile and S. Razin (ed.), The Mycoplasmas, vol. I. Academic Press, New York, N.Y.

    Chapter  Google Scholar 

  7. Boguslavsky, S., D. Menaker, I. Lysnyansky, T. Liu, S. Levisohn, R. Rosengarten, M. Garcia, and D. Yogev. 2000. Molecular characterization of the Mycoplasma gallisepticum pvpA gene which encodes a putative variable cytadhesin protein. Infect. Immun. 68:3956–3964.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  8. Bredt, W. 1968. Motility and multiplication of Mycoplasma pneumoniae: a phase contrast study. Pathol. Microbiol. 32:321–326.

    CAS  Google Scholar 

  9. Chambaud, I., R. Heilig, S. Ferris, V. Barbe, D. Samson, F. Galisson, I. Moszer, K. Dybvig, H. Wrobleski, A. Viari, E. P. Rocha, and A. Blanchard. 2001. The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis. Nucleic Acids Res. 29:2145–2153.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  10. Chen, C., J. Ma, A. Lazic, M. Backovic, K. J. Colley. 2000. Formation of insoluble oligomers correlates with ST6Gal I stable localization in the golgi. J. Biol. Chem. 275:13819–13826.

    Article  PubMed  CAS  Google Scholar 

  11. Dallo, S. F., A. Chavoya, and J. B. Baseman. 1990. Characterization of the gene for a 30-kilodalton adhesin-related protein of Mycoplasma pneumoniae. Infect. Immun. 58:4163–4165.

    PubMed Central  PubMed  CAS  Google Scholar 

  12. Dallo, S. F., A. Chavoya, C.-J. Su, and J. B. Baseman. 1989. DNA and protein sequence homologies between the adhesins of Mycoplasma genitalium and Mycoplasma pneumoniae. Infect. Immun. 57:1059–1065.

    PubMed Central  PubMed  CAS  Google Scholar 

  13. Dallo, S. F., A. L. Lazzell, A. Chavoya, S. P. Reddy, J. B. Baseman. 1996. Biofunctional domains of the Mycoplasma pneumoniae P30 adhesin. Infect. Immun. 64:2595–2601

    PubMed Central  PubMed  CAS  Google Scholar 

  14. Dandekar, T., M. Huynen, J. T. Regula, B. Ueberle, C. U. Zimmermann, M. A. Andrade, T. Doerks, L. Sánchez-Pulido, B. Snel, M. Suyama, Y. P. Yuan, R. Herrmann, and P. Bork. 2000. Re-annotating the Mycoplasma pneumoniae genome sequence: adding value, function and reading frames. Nucleic Acids Res. 28:3278–3288.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  15. Dhandayuthapani, S., W. G. Rasmussen, and J. B. Baseman. 1999. Disruption of gene mg218 of Mycoplasma genitalium through homologous recombination leads to an adherence-deficient phenotype. Proc. Natl. Acad. Sci. USA 96:5227–5232.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  16. Dirksen, L. B., K. A. Krebes, and D. C. Krause. 1994. Phosphorylation of cytadherenee-accessory proteins in Mycoplasma pneumoniae. J. Bacteriol. 176:7499–7505.

    PubMed Central  PubMed  CAS  Google Scholar 

  17. Dirksen, L. B., T. Profit, H. Hilbert, H. Plagens, R. Herrmann, and D. C. Krause. 1996. Sequence analysis and characterization of the hmw gene cluster of Mycoplasma pneumoniae. Gene 171:19–25.

    Article  PubMed  CAS  Google Scholar 

  18. Dorigo-Zetsma, J. W., J. Dankert, and S. A. J. Zaat. 2000. Genotyping of M pneumoniae clinical isolates reveals eight PI subtypes within two genomic groups. J. Clin. Microbiol. 38:965–970.

    PubMed Central  PubMed  CAS  Google Scholar 

  19. Dubreuil, R. R., and T. Grushko. 1998. Genetic studies of spectrin: new life for a ghost protein. Bioessays 20:875–878.

    Article  PubMed  CAS  Google Scholar 

  20. Feldner, J., U. Göbel, and W. Bredt. 1982. Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody. Nature (London) 298:765–767.

    Article  CAS  Google Scholar 

  21. Fisseha, M., H. H. Göhlmann, R. Herrmann, and D. C. Krause. 1999. Identification and complementation of frameshift mutations associated with loss of cytadherence in Mycoplasma pneumoniae. J. Bacteriol. 181:4404–4410.

    PubMed Central  PubMed  CAS  Google Scholar 

  22. Franzoso, G., P.-C. Hu, G. A. Meloni, and M. F. Barile. 1993. The immunodominant 90-kilodalton protein is localized on the terminal tip structure of Mycoplasma pneumoniae. Infect. Immun. 61:1523–1530.

    PubMed Central  PubMed  CAS  Google Scholar 

  23. Fraser, C. M., J. D. Gocayne, O. White, M. D. Adams, R. A. Clayton, R. D. Fleischmann, C. J. Bult, A. R. Kerlavage, G. Sutton, J. M. Kelley, J. L. Fritchman, J. F. Weidman, K. V. Small, M. Sandusky, J. Fuhrmann, D. Nguyen, T. R. Utterback, D. M. Saudek, C. A. Phillips, J. M. Merrick, J.-F. Tomb, B. A. Dougherty, K. F. Bott, P.-C. Hu, T. S. Lucier, S. N. Peterson, H. O. Smith, C.A. Hutchison H.I, and J. C. Venter. 1995. The minimal gene complement of Mycoplasma genitalium. Science 270:397–403.

    Article  PubMed  CAS  Google Scholar 

  24. Fulton, A. 1984. The Cytoskeleton. p. 7–8. Chapman and Hall, New York, N. Y.

    Google Scholar 

  25. Gavrilova, L. P., N. M. Rutkevitch, V. I. Gelfand, L. P. Motuz, J. Stahl, U. A. Bommer, and H. Bielka. 1987. Immunofluorescent localization of protein synthesis components in mouse embryo fibroblasts. Cell Biol.Int. Rep. 11:745–753.

    Article  PubMed  CAS  Google Scholar 

  26. Gevaert, K., and J. Vandekerckhove. 2000. Protein identification methods in proteomics. Electrophoresis 21:1145–1154.

    Article  PubMed  CAS  Google Scholar 

  27. Glass, J. I., E. J. Lefkowitz, J. S. Glass, C. R. Heiner, E. Y. Chen, and G. H. Cassell. 2000. Nature 407:757–762.

    Article  PubMed  CAS  Google Scholar 

  28. Göbel, U., V. Speth, and W. Bredt. 1981. Filamentous structures in adherent Mycoplasma pneumoniae cells treated with nonionic detergents. J. Cell Biol. 91:537–543.

    Article  PubMed  Google Scholar 

  29. Goh, M. S., T. S. Gorton, M. H. Forsyth, K. E. Troy, and S. J. Geary. 1998. Molecular and biochemical analysis of a 105 kDa Mycoplasma gallisepticum cytadhesin (GapA). Microbiology (U. K.) 144:2971–2978.

    Article  CAS  Google Scholar 

  30. Goloubinoff, P., A. Mogk, A. P. Zvi, T. Tomoyasu, and B. Bukau. 1999. Sequential mechanism of solubilization and refolding of stable protein aggregates by a bichaperone network. Proc. Natl. Acad. Sci. USA 96:13732–13737.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  31. Gottesman, S. 1996. Proteases and their targets in Escherichia coli. Annu. Rev. Genet. 30:465–506.

    Article  PubMed  CAS  Google Scholar 

  32. Hahn, T.-W., K. A. Krebes, and D. C. Krause. 1996. Expression in Mycoplasma pneumoniae of the recombinant gene encoding the cytadherence-accessory protein HMW1 and identification of HMW4 as a product. Mol. Microbiol. 19:1085–1093.

    Article  PubMed  CAS  Google Scholar 

  33. Hahn, T.-W., M. J. Willby, and D. C. Krause. 1998. HMW1 is required for cytadhesin P1 trafficking to the attachment organelle in Mycoplasma pneumoniae. J. Bacteriol. 180:1270–1276.

    PubMed Central  PubMed  CAS  Google Scholar 

  34. Hedreyda, C. T., and D. C. Krause. 1995. Identification of a possible cytadherence regulatory locus in Myooplasm pneumoniae. Infect. Immun. 63:3479–3483.

    PubMed Central  PubMed  CAS  Google Scholar 

  35. Himmelreich, R., H. Hubert, H. Plagens, E. Pirkl, B.-C. Li, and R. Herrmann. 1996. Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae. Nucleic Acids Res. 24:4420–4449.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  36. Hnatow, L. L., C. L. Keeler Jr., L. L. Tesmer, K. Czymmek, and J. E. Dohms. 1998. Characterization of MGC2, a Mycoplasma gallisepticum cytadhesin with homology to the Mycoplasma pneumoniae 30-kilodalton protein and Mycoplasma genitalium P32. Infect. Immun. 66:3436–3442.

    PubMed Central  PubMed  CAS  Google Scholar 

  37. Hooper, N. M. 1999. Detergent-insoluble glycosphingolipid/cholesterol-rich membrane domains, lipid rafts and caveolae. Mol. Membr. Biol. 16:145–156.

    Article  PubMed  CAS  Google Scholar 

  38. Hu, P.-C., R. M. Cole, Y. S. Huang, T. A. Graham, D. E. Gardner, A. M. Collier, and W. A. Clyde. 1982. Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science 216:313–315.

    Article  PubMed  CAS  Google Scholar 

  39. Hu, P.-C., A. M. Collier, and J. B. Baseman. 1977. Surface parasitism by Mycoplasma pneumoniae of respiratory epithelium. J. Exp. Med. 145:1328–1343.

    Article  PubMed  CAS  Google Scholar 

  40. Hu, P.-C., U. Schaper, A. M. Collier, W. A. Clyde Jr., M. Horikawa, Y. Huang, and M. F. Barile. 1987. A Mycoplasma genitalium protein resembling the Mycoplasma pneumoniae attachment protein P1. Infect. Immun. 55:1126–1131.

    PubMed Central  PubMed  CAS  Google Scholar 

  41. Inamine, J. M., T. P. Denny, S. Loechel, U. Schaper, C.-H. Huang, K. F. Bott, and P.-C Hu. 1988a. Nucleotide sequence of the P1 attachment protein gene of Mycoplasma pneumoniae. Gene 64: 217–219.

    Article  PubMed  CAS  Google Scholar 

  42. Inamine, J. M., S. Loechel, and P.-C. Hu. 1988b. Analysis of the nucleotide sequence of P1 Operon of Mycoplasma pneumoniae. Gene 73:175–183.

    Article  PubMed  CAS  Google Scholar 

  43. Jacobs, E. 1991. Mycoplasma pneumoniae virulence factors and the immune response. Rev. Med. Microbiol. 2:83–90.

    Google Scholar 

  44. Jacobs, E., K. Fuchte, and W. Bredt. 1987. Amino acid sequence and antigenicity of the amino-terminus of the 168 kDa adherence protein of Mycoplasma pneumoniae. J. Gen. Microbiol. 133:2233–2236.

    PubMed  CAS  Google Scholar 

  45. Jordan, J. L., K. M. Berry, M. F. Balish, and Duncan C. Krause. 2001 Stability and subcellular localization of cytadherence — associated protein P65 in Mycoplasma pneumoniae. J. Bacteriol. 183:7387–7391.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  46. Kahane, I., S. Tucker, D. K. Leith, J. Morrison-Plummer, and J. B. Baseman. 1985. Detection of the major adhesin P1 in triton shells of virulent Mycoplasma pneumoniae. Infect. Immun. 50:944–946.

    PubMed Central  PubMed  CAS  Google Scholar 

  47. Kenri, T., R. Taniguchi, Y. Sasaki, N. Okazaki, M. Narita, K. Izumikawa, M. Umetsu, and T. Sasaki. 1999. Identification of a new variable sequence in the P1 cytadhesin gene of Mycoplasma pneumoniae; evidence for the generation of antigenic variation by DNA recombination by repetitive sequences. Infect. Immun. 67:4557–4562.

    PubMed Central  PubMed  CAS  Google Scholar 

  48. Kihara, A., Y. Akiyama, and K. Ito. 1995. FtsH is required for proteolytic elimination of uncomplexed forms of SecY, an essential protein translocase subunit. Proc. Natl. Acad. Sci. USA 92:4532–4536.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  49. Krause, D. C. 1996. Mycoplasma pneumoniae cytadherence: unravelling the tie that binds. Mol. Microbiol. 20:247–253.

    Article  PubMed  CAS  Google Scholar 

  50. Krause, D.C. 1998. Mycoplasma pneumoniae cytadherence: organization and assembly of the attachment organelle. Trends Microbiol. 6:15–18.

    Article  Google Scholar 

  51. Krause, D. C., and M. F. Balish. 2001. Structure, function, and assembly of the terminal organelle of Mycoplasma pneumoniae. FEMS Microbiol. Lett. 198:1–7.

    Article  PubMed  CAS  Google Scholar 

  52. Krause, D. C., D. K. Leith, R. M. Wilson, and J. B. Baseman. 1982. Identification of Mycoplasma pneumoniae proteins associated with hemadsorption and virulence. Infect. Immun. 35:809–817.

    PubMed Central  PubMed  CAS  Google Scholar 

  53. Krause, D. C., T. Proft, C. T. Hedreyda, H. Hilbert, H. Piagens, R. Herrmann. 1997. Transposon mutagenesis reinforces the correlation between Mycoplasma pneumoniae cytoskeletal protein HMW2 and cytadherence. J. Bacteriol. 179:2668–2677.

    PubMed Central  PubMed  CAS  Google Scholar 

  54. Kravanja, M., R. Engelmann, V. Dossonnet, M. Bluggel, H. E. Meyer, R. Frank, A. Galinier, J. Deutscher, N. Schnell, and W. Hengstenberg. 1999. The hprK gene of Enterococcus faecalis encodes a novel bifunctional enzyme: the HPr kinase/phosphatase. Mol. Microbiol. 31:59–66.

    Article  PubMed  CAS  Google Scholar 

  55. Krebes, K. A., L. B. Dirksen, and D. C. Krause. 1995. Phosphorylation of Mycoplasma pneumoniae cytadherence-accessory proteins in cell extracts. J. Bacteriol. 177:4571–4574.

    PubMed Central  PubMed  CAS  Google Scholar 

  56. Layh-Schmitt, G., and M. Harkenthal. 1999. The 40- and 90-kDa membrane proteins (ORF6 gene product) of Mycoplasma pneumoniae are responsible for the tip structure formation and P1 (adhesin) associateion with the triton shell. FEMS Microbiol. Lett. 174:143–149.

    Article  PubMed  CAS  Google Scholar 

  57. Layh-Schmitt, G., and R. Herrmann. 1992. Localization and biochemical characterization of the ORF6 gene product of the Mycoplasma pneumoniae P1 operon. Infect. Immun. 60:2904–2913.

    Google Scholar 

  58. Layh-Schmitt, G., and R. Herrmann. 1994. Spatial arrangement of gene products of the P1 operon in the membrane of Mycoplasma pneumoniae. Infect. Immun. 62:974–979.

    PubMed Central  PubMed  CAS  Google Scholar 

  59. Layh-Schmitt, G., H. Hubert, and E. Pirkl. 1995. A spontaneous hemadsorption-negative mutant of Mycoplasma pneumoniae exhibits a truncated adhesin-related 30-kilodalton protein and lacks the cytadherence-accessory protein HMW1. J. Bacteriol. 177:843–846.

    PubMed Central  PubMed  CAS  Google Scholar 

  60. Layh-Schmitt, G., R. Himmelreich, and U. Leibfried. 1997. The adhesin related 30-kDa protein of Mycoplasma pneumoniae exhibits size and antigen variability. FEMS Microbiol. Lett. 152:101–8.

    Article  PubMed  CAS  Google Scholar 

  61. Layh-Schmitt, G., A. Podtelejnikov, and M. Mann. 2000. Proteins complexed to the P1 adhesin of Mycoplasma pneumoniae. Microbiology (UK) 146:741–747.

    CAS  Google Scholar 

  62. London, E., and D. A. Brown. 2000. Insolubility of lipids in Triton X-100: physical origin and relationship to sphingolipid/cholesterol membrane domains (rafts). Biochim. Biophys. Acta 1508:182–195.

    Article  PubMed  CAS  Google Scholar 

  63. Lu, C., M. Reedy, and H. P. Erickson. 2000. Straight and curved conformations of FtsZ are regulated by GTP hydrolysis. J. Bacteriol. 182:164–170.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  64. Margolin, W. 2000. Themes and variations in prokaryotic cell division. FEMS Microbiol. Rev. 24:531–548.

    Article  PubMed  CAS  Google Scholar 

  65. Masters, C. J., S. Reid, and M. Don. 1987. Glycolysis: new concepts in an old pathway. Mol. Cell Biochem. 76:3–14.

    Article  PubMed  CAS  Google Scholar 

  66. Mattevi, A., G. Obmolova, E. Schulze, K. H. Kalk, A. H. Westphal, A. de Kok, and W. G. Hoi. 1992. Atomic structure of the cubic core of the pyruvate dehydrogenase multienzyme complex. Science. 255:1544–1550.

    Article  PubMed  CAS  Google Scholar 

  67. Matus, A. I., and D. H. Taff-Jones. 1978. Morphology and molecular composition of isolated postsynaptic junctional structures. Proc. R. Soc. Lond. B. Biol. Sci. 203:135–151.

    Article  PubMed  CAS  Google Scholar 

  68. Meng, K. E., and R. M. Pfister. 1980. Intracellular structures of Mycoplasma pneumoniae revealed after membrane removal. J. Bacteriol. 144:390–399.

    PubMed Central  PubMed  CAS  Google Scholar 

  69. Mernaugh, G. R., S. F. Dallo, S. C. Holt, and J. B. Baseman. 1993. Properties of adhering and nonadhering populations of Mycoplasma genitalium. Clin. Infect. Dis. 17:S69-S78.

    Article  Google Scholar 

  70. Morrison-Plummer, J., D. K. Leith, and J. B. Baseman. 1986. Biological effects of anti-lipid and anti-protein monoclonal antibodies on Mycoplasma pneumoniae. Infect. Immun. 53:398–403.

    PubMed Central  PubMed  CAS  Google Scholar 

  71. Neimark, H. 1977. Extraction of an actin-like protein from the prokaryote Mycoplasma pneumoniae. Proc. Natl. Acad. Sci. USA 74:4041–4045.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  72. Norris, V., G. Turnock, and D. Sigee. 1996. The Escherichia coli enzoskeleton. Mol. Microbiol. 19:197–204.

    Article  PubMed  CAS  Google Scholar 

  73. Ogle, K. F., K. K. Lee, and D. C. Krause. 1992. Nucleotide sequence analysis reveals novel features of the phase-variable cytadherence accessory protein HMW3 of Mycoplasma pneumoniae. Infect. Immun. 60:1633–1641.

    PubMed Central  PubMed  CAS  Google Scholar 

  74. Papazisi, L., K. E. Troy, T. S. Gorton, X. Liao, and S. J. Geary. 2000. Analysis of cytadherence-deficient, GapA-negative Mycoplasma gallisepticum strain R. Infect. Immun. 12:6643–6649.

    Article  Google Scholar 

  75. Piatigorsky, J. 1998. Multifunctional lens crystallins and corneal enzymes: more than meets the eye. Ann. N. Y. Acad. Sci. 842:7–15.

    Article  PubMed  CAS  Google Scholar 

  76. Popham, P. L., T.-W. Hahn, K. A. Krebes, and D. C. Krause. 1997. Loss of HMW1 and HMW3 in noncytadhering mutants of Mycoplasma pneumoniae occurs post-translationally. Proc. Natl. Acad. Sci. USA 94:13979–13984.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  77. Proft, T., and R. Herrmann. 1994. Identification and characterization of hitherto unknown Mycoplasma pneumoniae proteins. Mol. Microbiol. 13: 337–348.

    Article  PubMed  CAS  Google Scholar 

  78. Proft, T., H. Hilbert, G. Layh-Schmitt, and R. Herrmann. 1995. The proline-rich P65 protein of Mycoplasma pneumoniae is a component of the Triton X-100-insoluble fraction and exhibits size polymorphism in the strains M129 and FH. J. Bacteriol. 177:3370–3378.

    PubMed Central  PubMed  CAS  Google Scholar 

  79. Proft, T., H. Hilbert, H. Plagens, and R. Herrmann. 1996. The P200 protein of Mycoplasma pneumoniae shows common features with the cytadherence-associated proteins HMW1 and HMW3. Gene. 171: 79–82.

    Article  PubMed  CAS  Google Scholar 

  80. Razin, S., and E. Jacobs. 1992. Mycoplasma adhesion. J. Gen. Microbiol. 138: 407–422.

    Article  PubMed  CAS  Google Scholar 

  81. Reddy, S. P., W. G. Rasmussen, and J. B. Baseman. 1995. Molecular cloning and characterization of an adherence-related operon of Mycoplasma genitalium. J. Bacteriol. 177:5943–5951.

    PubMed Central  PubMed  CAS  Google Scholar 

  82. Regula, J. T., G. Boguth, A. Görg, J. Hegermann, F. Mayer, R. Frank, and R. Herrmann. 2001. Defining the mycoplasma ‘cytoskeleton’: the protein componsition of the Triton X-100 insoluble fraction of the bacterium Mycoplasma pneumoniae determined by 2-D gel electrophoresis and mass spectrometry. Microbiology (U. K.) 147:1045–1057.

    CAS  Google Scholar 

  83. Regula, J. T., B. Ueberle, G. Boguth, A. Görg, M. Schnolzer, R. Herrmann, and R. Frank. 2000. Towards a two-dimensional proteome map of Mycoplasma pneumoniae. Electrophoresis 21:3765–3780.

    Article  PubMed  CAS  Google Scholar 

  84. Reizer, J., C. Hoischen, F. Titgemeyer, C. Rivolta, R. Rabus, J. Stulke, D. Karamata, M. H. Saier Jr, and W. Hillen. 1998. A novel protein kinase that controls carbon catabolite repression in bacteria. Mol. Microbiol. 27:1157–1169.

    Article  PubMed  CAS  Google Scholar 

  85. Romero-Arroyo, C. E., J. Jordan, S. J. Peacock, M. J. Willby, M. A. Farmer, and D. C. Krause. 1999. Mycoplasma pneumoniae protein P30 is required for cytadherence and associated with proper cell development. J. Bacteriol. 181:1079–1087.

    PubMed Central  PubMed  CAS  Google Scholar 

  86. Rothfield, L. L., S. Justice, J. García-Lara. 1999. Bacterial cell division. Annu. Rev. Genet. 33:423–448.

    Article  PubMed  CAS  Google Scholar 

  87. Schliwa, M. 1986. The cytoskeleton: an introductory survey, p. 1–2. In M. Alfert, W. Beermann, L. Goldstein, and K. R. Porter (ed.), Cell Biology Monographs, vol. XIII. Springer-Verlag, New York, N. Y.

    Google Scholar 

  88. Schumann, W. 1999. FtsH — a single-chain charonin? FEMS Microbiol. Rev. 23:1–11.

    PubMed  CAS  Google Scholar 

  89. Seto, S., G. Layh-Schmitt, T. Kenri, and M. Miyata. 2001. Visualization of the attachment organelle and cytadherence proteins of Mycoplasma pneumoniae by immunofluorescence microscopy. J. Bacteriol. 183:1621–1630.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  90. Sperker, B., P.-C. Hu, and R. Herrmann. 1991. Identification of gene products of the P1 Operon of Mycoplasma pneumoniae. Mol. Microbiol. 5:299–306.

    Article  PubMed  CAS  Google Scholar 

  91. Stevens, M. K., and D. C. Krause. 1991. Localization ofthe Mycoplasma pneumoniae cytadherence-accessory proteins HMW1 and HMW4 in the cytoskeletonlike triton shell. J. Bacteriol. 173:1041–1050.

    PubMed Central  PubMed  CAS  Google Scholar 

  92. Stevens, M. K., and D. C. Krause. 1992. Mycoplasma pneumoniae cytadherence phase-variable protein HMW3 is a component of the attachment organelle. J. Bacteriol. 174:4265–4274.

    PubMed Central  PubMed  CAS  Google Scholar 

  93. Su, C.-J., A. Chavoya, and J. B. Baseman. 1989. Spontaneous mutation results in loss of the cytadhesin (P1) of Mycoplasma pneumoniae. Infect. Immun. 57:3237–3239.

    PubMed Central  PubMed  CAS  Google Scholar 

  94. Su, C.-J., A. Chavoya, S. F. Dallo, and J. B. Baseman. 1990. Sequence divergence of the cytadhesin gene of Mycoplasma pneumoniae. Infect. Immun. 58:2669–2674.

    PubMed Central  PubMed  CAS  Google Scholar 

  95. Su, C.-J., V. V. Tryon, and J. B. Baseman. 1987. Cloning and sequence analysis of cytadhesin P1 gene from Mycoplasma pneumoniae. Infect. Immun. 55:3023–3029.

    PubMed Central  PubMed  CAS  Google Scholar 

  96. Sumegi, B., Z. Liposits, L. Inman, W. K. Pauli, and P. A. Srere. 1987. Electron microscopic study on the size of pyruvate dehydrogenase complex in situ. Eur. J. Biochem. 169:223–230.

    Article  PubMed  CAS  Google Scholar 

  97. Tham, T. N., S. Ferris, E. Bahraoui, S. Canarelli, L. Montagnier, and A. Blanchard. 1994. Molecular characterization of the P1-like adhesin gene from Mycoplasma pirum. J. Bacteriol. 176:781–788.

    PubMed Central  PubMed  CAS  Google Scholar 

  98. Waldo, R. H. III, P. L. Popham, C. E. Romero-Arroyo, E. A. Mothershed, K. K. Lee, and D. C. Krause. 1999. Transcriptional analysis of the hmw gene cluster of Mycoplasma pneumoniae. J. Bacteriol. 181:4978–4985.

    PubMed Central  PubMed  CAS  Google Scholar 

  99. Zhang, G., V. Gurtu, and S. R. Kain. 1996. An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochem. Biophys. Res. Comm. 227:707–711

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, University of Georgia, Athens, GA, USA

    Mitchell F. Balish & Duncan C. Krause

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  1. Mitchell F. Balish
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  2. Duncan C. Krause
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Editors and Affiliations

  1. The Hebrew University-Hadassah Medical School, Jerusalem, Israel

    Shmuel Razin

  2. University of Heidelberg, Heidelberg, Germany

    Richard Herrmann

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© 2002 Springer Science+Business Media New York

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Balish, M.F., Krause, D.C. (2002). Cytadherence and the Cytoskeleton. In: Razin, S., Herrmann, R. (eds) Molecular Biology and Pathogenicity of Mycoplasmas. Springer, Boston, MA. https://doi.org/10.1007/0-306-47606-1_22

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  • DOI: https://doi.org/10.1007/0-306-47606-1_22

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-8232-5

  • Online ISBN: 978-0-306-47606-8

  • eBook Packages: Springer Book Archive

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