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Genetic Mechanisms of Surface Variation

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Molecular Biology and Pathogenicity of Mycoplasmas

Abstract

The mycoplasma cell surface is unique among prokaryotes and is highly evolved to accommodate the lifestyle of these diverse organisms during interaction with their respective vertebrate and plant hosts. Particularly in the absence of a cell wall, several adaptive features must be in place to support the survival of these organisms during transmission and residence in varied niches, both extracellular and in some cases within host cells (22, 24, 68). As with any pathogenic organism, the mycoplasma surface reflects the functional interaction of individual organisms with host compartments, including interactions with the innate and adaptive immune systems. Quite unlike other bacteria, however, all functions are expressed from remarkably limited gene sets that are rapidly becoming delineated through mycoplasma genome sequencing projects (11, 26, 28, 36). Insights from these projects as well as many experimental studies now reveal one striking aspect of mycoplasmal biology: the prevalence of mutation-based systems as an adaptive strategy for survival. In contrast to their eubacterial counterparts, mycoplasmal genomes so far have revealed few of the complex systems for classic gene regulation and environmental sensing that endow adaptive flexibility for individual bacterial cells.

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References

  1. Alen, C., D. J. Sherratt, and S. D. Colloms. 1997. Direct interaction of aminopeptidase A with recombination site DNA in Xer site-specific recombination. EMBO J. 16:5188–5197.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  2. Baseggio, N., M. D. Glew, P. F. Markham, K. G. Whithear, and G. F. Browning. 1996. Size and genomic location of the pMGA multigene family of Mycoplasma gallisepticum. Microbiology 142:1429–1435.

    Article  PubMed  CAS  Google Scholar 

  3. Bath, J., D. J. Sherratt, and S. D. Colloms, 1999. Topolgy of Xer recombination on catenanes produced by Lambda integrase. J. Mol. Biol. 289:873–883.

    Article  PubMed  CAS  Google Scholar 

  4. Behrens, A., M. Heller, H. Kirchhoff, D. Yogev, and R. Rosengarten. 1994. A family of phase-and size-variant membrane surface lipoprotein antigens (Vsps) of Mycoplasma bovis. Infect. Immun. 62:5075–5084.

    PubMed Central  PubMed  CAS  Google Scholar 

  5. Bhugra, B., and K. Dybvig. 1992. High-frequency rearrangements in the chromosome of Mycoplasma pulmonis correlate with phenotypic switching. Mol. Microbiol. 6:149–1154.

    Article  Google Scholar 

  6. Bhugra, B., L. L. Voelker, N. Zou, H. Yu, and K. Dybvig. 1995. Mechanism of antigenic variation in Mycoplasma pulmonis: interwoven, site-specific DNA inversions. Mol. Microbiol. 18:703–714.

    Article  PubMed  CAS  Google Scholar 

  7. Boesen, T., J. Emmerson, L. T. Jensen, S. A. Ladefoged, P. Thorsen, S. Birkelund, and G. Christiansen. 1998. The Mycoplasma hominis vaa gene displays a mosaic gene structure. Mol. Microbiol. 29:97–110.

    Article  PubMed  CAS  Google Scholar 

  8. Boguslavsky, S., D. Menaker, Lysnyansky, I. T. Liu, S. Levisohn, R. Rosengarten, M. Garcia, and D. Yogev. 2000. Molecular characterization of the Mycoplasma gallisepticum pvpA gene which encodes a putative variable cytadhesin protein. Infect. Immun. 68: 3956–3964.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  9. Borst, P., and D. R. Greaves. 1987. Programmed gene rearrangements altering gene expression. Science. 235:658–667.

    Article  PubMed  CAS  Google Scholar 

  10. Calcutt, M. J., M. F. Kim, A.B. Karpas, P.F. Mueand K.S. Wise. 1999. Differential posttranslational processing confers intraspecies variation of a major surface lipoprotein and a macrophage-activating lipopeptide of Mycoplasma fermentans Infect. Immun. 67: 760–771.

    CAS  Google Scholar 

  11. Chambaud, I., R. Heilig, S. Ferris, V. Barbe, D. Samson, F. Galisson, I. Moszer, K. Dybvig, H. Wroblewski, A. Viari, E. P. C. Rocha, and A. Blanchard. 2001. The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis. Nucleic Acids Res. 29:2145–2153.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  12. Christiansen, G., S. L. Mathiesen., C. Nyvold, and S. Birkelund. 1994. Analysis of a Mycoplasma hominis membrane protein, P120. FEMS Microbiol. Lett. 121:121–128.

    Article  PubMed  CAS  Google Scholar 

  13. Citti, C., and K. S. Wise. 1995. Mycoplasma hyorhinis vlp gene transcription: critical role in phase variation and expression of surface lipoproteins. Mol. Microbiol. 18:649–660.

    Article  PubMed  CAS  Google Scholar 

  14. Citti, C., M. F. Kim, and K. S. Wise. 1997. Elongated versions of Vlp surface lipoproteins protect Mycoplasma hyorhinis escape variants from growth-inhibiting host antibodies. Infect.Immun. 65:1773–1785.

    PubMed Central  PubMed  CAS  Google Scholar 

  15. Citti, C., Watson-McKown , Droesse, M., and K. S. Wise. 2000. Gene families encoding phase- and size- variable surface lipoproteins of Mycoplasma hyorhinis. J. Bacteriol. 182:1356–1363.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  16. Colloms, S. D., R. McCulloch, K. Grant, L. Neilson, and D. J. Sherratt. 1996. Xer-mediated site-specific recombination in viro. EMBO J. 15:1172–1181.

    PubMed Central  PubMed  CAS  Google Scholar 

  17. Dallo, S. F., A. Chavoya and J. B. Baseman. 1990. Characterization of the gene for a 30-kilodalton adhesin-related protein of Mycoplasma pneumoniae. Infect Immun. 58:4163–4165.

    PubMed Central  PubMed  CAS  Google Scholar 

  18. Davis, K. L. and K. S. Wise. 2002. Site-specific proteolysis of the MALP-404 lipoprotein determines the release of a soluble selective lipoprotein associated motif-containing fragment and alteration of the surface phenotype of Mycoplasma fermentans. Infect. Immun. 70(3):1129–1135.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  19. Dorigo-Zetsma, J. W., B. Wilbrink, J. Dankert and S. A. J. Zaat. 2001. Mycoplasma pneumoniae P1 type 1- and type 2-specific sequences within the P1 cytadhesin gene of individual strains. Infect. Immun. 69:5612–5618.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  20. Dybvig, K., J. Alderete, and G. H. Cassell. 1988. Adsorption of Mycoplasma pulmonis virus P1 to host cells. J. Bacteriol. 170:4373–4375.

    PubMed Central  PubMed  CAS  Google Scholar 

  21. Dybvig, K., J. K. Simecka, H. L. Watson, and G. H. Cassell. 1989. High-frequency variation in Mycoplasma pulmonis colony size. J. Bacteriol. 171:5165–5168.

    PubMed Central  PubMed  CAS  Google Scholar 

  22. Dybvig, K. 1990. Mycoplasmal genetics. Annu. Rev. Microbiol. 44: 81–104.

    Article  PubMed  CAS  Google Scholar 

  23. Dybvig, K. 1993. DNA rearrangements and phenotypic switching in prokaryotes. Mol. Microbiol. 10:465–471.

    Article  PubMed  CAS  Google Scholar 

  24. Dybvig, K., and L. L. Voelker. 1996. Molecular Biology of Mycoplasmas. Annu. Rev. Microbiol. 50:25–57.

    Article  PubMed  CAS  Google Scholar 

  25. Ferris, S., H. L. Watson, O. Neyrolles, L. Montagnier, and A. Blanchard. 1995. Characterization of a major Mycoplasma penetrans lipoprotein and its gene. FEMS Microbiol. Lett. 130:313–320.

    Article  PubMed  CAS  Google Scholar 

  26. Fraser, C. M., J. D. Gocayne, O. White, M. D. Adams, R. A. Clayton, R. D. Fleischmann, C. J. Bult, A. R. Kerlavage, G. Sutton, J. M. Kelley, J. L. Fritchman, J. F. Weidman, K. V. Small, M. Sandusky, J. Fuhrmann, D. Nguyen, T. R. Utterback, D. M. Saudek, C. A. Phillips, J. M. Merrick, J. F. Tomb, B. A. Dougherty, K. F. Bott, P. C. Hu, T. S. Lucier, S. N. Peterson, H. O. Smith, C. A. Hutchison, III, , and J. C. Venter. 1995. The minimal gene complement of Mycoplasma genitalium. Science 270:397–403.

    Article  PubMed  CAS  Google Scholar 

  27. Glasgow, A. C., K. T. Hughes, and M. I. Simon. 1989. Bacterial DNA inversion systems, p. 637–659. In E. Berg and M. M. Howe (ed.), Mobile DNA. American Society for Microbiology, Washington, D.C.

    Google Scholar 

  28. Glass, J. I., E. J. Lefkowitz, J. S. Glass, C. R. Heiner, E. Y. Chen, and G. H. Cassell. 2000. The complete sequence of the mucosal pathogen Ureaplasma urealyticum. Nature 407:757–762.

    Article  PubMed  CAS  Google Scholar 

  29. Glew, M. D., P. F. Markham, G. F. Browning, and I. D. Walker. 1995. Expression studies on four members of the pMGA multigene family in Mycoplasma gallisepticum. Microbiology 141:3005–3014.

    Article  PubMed  CAS  Google Scholar 

  30. Glew, M. D., P. F. Markham, G. F. Browning, and I. D. Walker. 1998. Variation in the GAA trinucleotide repeat lengths within the non-coding regions of Mycoplasma gallisepticum pMGA genes controls their expression. Infect. Immun. 66:5833–5841.

    PubMed Central  PubMed  CAS  Google Scholar 

  31. Glew, M. D., G. F. Browning, P. F. Markham, and I. D. Walker. 2000. pMGA phenotypic variation in Mycoplasma gallisepticum occurs in vivo and is mediated by trinucleotide repeat length variation. Infect. Immun. 68:6027–6033.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  32. Goh, M. S., T. S. Gorton, M. H. Forsyth, K. E. Troy, and S. J. Geary. 1998. Molecular and biochemical analysis of a 105 kDa Mycoplasma gallisepticum cytadhesin (GapA). Microbiology 144:2941–2950.

    Article  Google Scholar 

  33. Guhathakurta, A., I. Viney, and D. Summers. 1996. Accessory proteins impose site selectivity during ColE1 dimer resolution. Mol. Microbiol. 20:613–620.

    Article  PubMed  CAS  Google Scholar 

  34. Henrich, B., Feldmann , and U. Hadding. 1993. Cytoadhesins of Mycoplasma hominis. Infect. Immun. 61:2945–2951.

    PubMed Central  PubMed  CAS  Google Scholar 

  35. Henrich, B., A. Kitzerow, R. C. Feldmann, H. Schaal, and U. Hadding. 1996. Repetitive elements of the Mycoplasma hominis adhesin p50 can be differentiated by monoclonal antibodies. Infect. Immun. 64:4027–4034.

    PubMed Central  PubMed  CAS  Google Scholar 

  36. Himmelreich, R., H. Hilbert, H. Plagens, E. Pirkl, B. C. Li, and R. Herrmann. 1996. Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae. Nucleic Acids Res. 24:4420–4449.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  37. Hnatow, L. L., C. L. Keeler Jr, L. L. Tessmer, K. Czymmek and J. E. Dohms. 1998. Characterization of MGC2, a Mycoplasma gallisepticum cytadhesin with homology to the Mycoplasma pneumoniae 30-kilodalton protein P30 and Mycoplasma genitalium P32. Infect. Immun. 66:3436–3442.

    PubMed Central  PubMed  CAS  Google Scholar 

  38. Hsu, T. and C. Minion. 1998. Identification of the cilium binding epitope of the Mycoplasma hyopneuminiae P97 adhesin. Infect.Immun. 66:4762–4766.

    PubMed Central  PubMed  CAS  Google Scholar 

  39. Inamine, J. M., S. Loechel., A. M. Collier., R.F. Barile, and P. C. Hu. 1989. Nucleotide sequence of the MgPa (mgp) operon of Mycoplasma genitalium and comparison to the P1 (mpp) operon of Mycoplasma pneumoniae. Gene 82:259–267.

    Article  PubMed  CAS  Google Scholar 

  40. Keeler, C. L. Jr, L. L. Hnatow, P. L. Whetzel and J. E. Dohms. 1996. Cloning and characterization of a putative cytadhesin gene (mgc1) from Mycoplasma gallisepticum. Infect. Immun. 64:1541–7.

    PubMed Central  PubMed  Google Scholar 

  41. Kitten, T., A. V. Barrera, and A. G. Barbour. 1993. Intragenic recombination and a chimeric outer membrane protein in the relapsing fever agent Borrelia hermsii. J. Bacteriol. 175:2516–2522.

    PubMed Central  PubMed  CAS  Google Scholar 

  42. Romano Teruya. 1999. Shufflons: Mutiple inversion systems and integrons. Ann. Rev. Gen. 33: 171–191.

    Article  Google Scholar 

  43. Leigh, S. A. and Wise, K. S. Mycoplasma fermentans P29 surface lipoprotein is an adhesin. Abstr. 100th Ann. Meet. Amer. Soc. Microbiol. Abstract G-12, 344. 2000.

    Google Scholar 

  44. Leigh, S. A. 2002. Functional analysis of the Mycoplasma fermentans P29 adhesin. Ph.D. Thesis. University of Missouri-Columbia, Columbia, MO. Submitted.

    Google Scholar 

  45. Liu, L., K. Dybvig, V. S. Panangala, V. L. van Santen and C. T. French. 2000. GAA trinucleotide repeat region regulates M9/pMGA gene expression in Mycoplasma gallisepticum. Infect. Immun. 68:871–876.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  46. Lysnyansky, I., R. Rosengarten, and D. Yogev. 1996. Phenotypic switching of variable surface lipoproteins in Mycoplasma bovis involves high-frequency chromosomal rearrangements. J. Bacteriol. 178: 5395–5401.

    PubMed Central  PubMed  CAS  Google Scholar 

  47. Lysnyansky, I., K. Sachse, R. Rosenbusch, S. Levisohn, and D. Yogev. 1999. The vsp locus of Mycoplasma bovis: gene organization and structural features. J. Bacteriol. 181:5734–5741.

    PubMed Central  PubMed  CAS  Google Scholar 

  48. Lysnyansky, I., Y. Ron, and D. Yogev. 2001. Juxtaposition of an active promoter to vsp genes via site-specific DNA inversions generates antigenic variation in Mycoplasma bovis. J. Bacteriol. 183:5698–5708.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  49. Lysnyansky, I., Y. Ron., K. Sachse, and D. Yogev. 2001. Intrachromosomal recombination within the vsp locus of Mycoplasma bovis generates a chimeric variable surface lipoprotein antigen. Infect. Immun. 69:703–3712.

    Article  Google Scholar 

  50. Markham, P. F., M. D. Glew, M. R. Brandon, I. D. Walker, and K. G. Whithear. 1992. Characterization of a major hemagglutinin protein from Mycoplasma gallisepticum. Infect. Immun. 60:3885–3891.

    PubMed Central  PubMed  CAS  Google Scholar 

  51. Markham, P. F., M. D. Glew, K. G. Whithear, and I. D. Walker. 1993. Molecular cloning of a member of the gene family that encodes pMGA, a hemagglutinin of Mycoplasma gallisepticum. Infect. Immun. 61:903–909.

    PubMed Central  PubMed  CAS  Google Scholar 

  52. Markham, P. F., M. D. Glew, J. E. Sykes, T. R. Bowden, T. D. Pollocks, G. F. Browning, K. G. Whithear, and I. D. Walker. 1994. The organisation of the multigene family which encodes the major cell surface protein, pMGA, of Mycoplasma gallisepticum. FEBS Lett. 352:347–352.

    Article  PubMed  CAS  Google Scholar 

  53. Markham, P. F., M. D. Glew, G. F. Browning, K. G. Whithear, and I. D. Walker. 1998. Expression of two members of the pMGA gene family of Mycoplasma gallisepticum oscillates and is influenced by pMGA-specific antibodies. Infect. Immun. 66:2845–2853.

    PubMed Central  PubMed  CAS  Google Scholar 

  54. Markham, P. F., M. F. Duffy, M. D. Glew and G. F. Browning. 1999. A gene family in Mycoplasma imitans closely related to the pMGA family of Mycoplasma gallisepticum. Microbiology. 145:2095–2103.

    Article  PubMed  CAS  Google Scholar 

  55. Mernaugh, G. R., S.F. Dallo, S. C. Holt, and J. B. Baseman. 1993. Properties of adhering and nonadhering populations of Mycoplasma genitalium. Clin. Infect. Dis. 17 (Suppl. 1) S69-S78.

    Article  Google Scholar 

  56. Meyer, T. F., C. P. Gibbs, and R. Haas. 1990. Variation and control of protein expression in Neisseria. Annu. Rev. Microbiol. 44:451–477.

    Article  PubMed  CAS  Google Scholar 

  57. Moskowitz, I.P.G., K. A. Heichman, and R. C. Johnson. 1991. Alignment of recombination sites in Hin-mediated site-specific DNA recombination. Genes & Development 5:1635–1645.

    Article  CAS  Google Scholar 

  58. Noormohammadi, A.H., Markham, P.F., Whithear, K.G., Walker, I.D., Gurevich, V.A., Ley, D.H., and G. F. Browning. 1997 Mycoplasma synoviae has two distinct phase-variable major membrane antigens, one of which is a putative hemagglutinin. Infect. Immun. 65:2542–2547.

    PubMed Central  PubMed  CAS  Google Scholar 

  59. Noormohammadi, A.H., Markham, P.F., Duffy, M.F., Whithear, K.G., and Browning, G.F. 1998. Multigene families encoding the major hemagglutinins in phylogenetically distinct mycoplasmas. Infect. Immun. 66:3470–3475.

    PubMed Central  PubMed  CAS  Google Scholar 

  60. Noormohammadi, A. H., P. F. Markham, A. Kanci, K. G. Whithear and G. F. Browning. 2000. A novel mechanism for control of antigenic variation in the haemagglutinin gene family of Mycoplasma synoviae. Mol. Microbiol. 35:911–923.

    Article  PubMed  CAS  Google Scholar 

  61. Nussboum, S., I. Lysnyansky, K. Sachse, S. Levisohn and D. Yogev. 2002. Extended Repertoire of Genes Encoding Variable Surface Lipoproteins (Vsps) in Mycoplasma bovis Strains Infect. Immun. 70: In Press.

    Google Scholar 

  62. Nyvold, C., S. Birkelund, and G. Christiansen. 1997. The Mycoplasma homonis PG21 contains multiple directly repeated sequences. Infect. Immun. 63:212–223.

    Google Scholar 

  63. Olson, L.D., S.W. Shane, A.A. Karpas, T.M. Cuningham, P.S. Probst and M.F. Barile. 1991. Monoclonal antibodies to surface antigens of a pathogenic Mycoplasma hominis strain. Infect. Immun. 59:1683–1689.

    PubMed Central  PubMed  CAS  Google Scholar 

  64. Pays, E. 1989. Pseudogenes, chimaeric genes and the timing of antigen variation in African trypanosomes. Trends. Genet. 5:389–391.

    Article  PubMed  CAS  Google Scholar 

  65. Peterson, S. N., C. C. Bailey, J. S. Jensen, M. B. Borre, E. S. King, K. F. Bott, and C. A. Hutchison, III. 1995. Characterization of repetitive DNA in the Mycoplasma genitalium genome: possible role in the generation of antigenic variation. Proc. Natl. Acad. Sci. USA 92:11829–11833.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  66. Petes, T. D., and C. W. Hill. 1988. Recombination between repeated genes in microorganisms. Annu. Rev. Genet. 22:147–168.

    Article  PubMed  CAS  Google Scholar 

  67. Rainey, P. B., E. R. Moxon, and I. P. Thompson. 1993. Intraclonal polymorphism in bacteria. Adv. Microb. Ecol. 13:263–300.

    Article  Google Scholar 

  68. Razin, S., D. Yogev, and Y. Naot. 1998. Molecular biology and pathogenicity of mycoplasmas. Microbiol. Mol. Biol. Rev. 62:1094–1156.

    PubMed Central  PubMed  CAS  Google Scholar 

  69. Reddy, S. P., W. G. Rasmussen, and J. B. Baseman. 1995. Molecular cloning and characterization of an adherence-related Operon of Mycoplasma genitalium. J. Bacteriol. 177:5943–5951.

    PubMed Central  PubMed  CAS  Google Scholar 

  70. Robertson, B. D., and T. F. Meyer. 1992. Antigenic variation in bacterial pathogens, p. 61–73. In C. W. Hormaeche, C. W. Penn and C. J. Smyth (ed.), Molecular Biology of Bacterial Infection, Vol. 49. Cambridge University Press.

    Google Scholar 

  71. Röske, K., A. Blanchard, I. Chambaud, C. Citti, J. H. Helbig, M. C. Prevost, R. Rosengarten, and E. Jacobs. 2001. Phase variation among major surface antigens of Mycoplasma penetrans. Infect.Immun. 69:7642–7651.

    Article  PubMed Central  PubMed  Google Scholar 

  72. Rosengarten, R. and K. S. Wise. 1990. Phenotypic switching in mycoplasmas: Phase variation of diverse surface lipoproteins. Science. 247:315–318.

    Article  PubMed  CAS  Google Scholar 

  73. Rosengarten, R. and K. S. Wise. 1991. The Vlp system of Mycoplasma hyorhinis: combinatorial expression of distinct size variant lipoproteins generating high-frequency surface antigenic variation. J. Bacteriol. 173:4782–4793.

    PubMed Central  PubMed  CAS  Google Scholar 

  74. Ruland, K., R. Himmelreich, and R. Herrmanm. 1994. Sequence divergence in the ORF6 gene of Mycoplasma pneumoniae. J. Bacteriol. 176:5302–5209.

    Google Scholar 

  75. Sachse, K., J. H. Helbig, I. Lysnyansky, C. Grajetzki, W. Muller, E. Jacobs, and D. Yogev. 2000. Epitope mapping of immunogenic and adhesive structures in repetitive domains of Mycoplasma bovis variable surface lipoproteins. Infect. Immun. 68:680–687.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  76. Saunders, J. R. 1986. Genetic basis of phase and antigenic variation in bacteria, p. 57–76. In T. H. Birkbeck and C. W. Penn (ed.), Antigenic Variation in Infectious Diseases. Society for General Microbiology, IRL Press, Oxford.

    Google Scholar 

  77. Seifert, H. S., and M. So. 1988. Genetic mechanisms of bacterial antigenic variation. Microbiol. Rev. 52:327–336.

    PubMed Central  PubMed  CAS  Google Scholar 

  78. Shen, X., J. Gumulak, H. Yu, C. Todd French, N. Zou, and K. Dybvig. 2000. Gene rearrangements in the vsa locus of Mycoplasma pulmonis. J. Bacteriol. 182: 2900–2908.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  79. Simecka, J. W., J. K. Davis, M. K. Davidson, S. E. Ross, C. T. K.-H. Stadtländer, and G. H. Cassell. 1992. Mycoplasma diseases of animals, p. 391–416. In J. Maniloff, R. N. McElhaney, L. R. Finch and J. B. Baseman (ed.), Mycoplasmas: Molecular Biology and Pathogenesis. American Society for Microbiology, Washington, D.C.

    Google Scholar 

  80. Simmons, W. L., C. Zuhua, J.I. Glass, J.W. Simecka, G. H. Cassell, and H.L. Watson. 1996. Sequence analysis of the chromosomal region around and within the V-1-encoding gene of Mycoplasma pulmonis: Evidence for DNA inversion as a mechanism for V-l variation. Infect. Immun. 64:472–479.

    PubMed Central  PubMed  CAS  Google Scholar 

  81. Smith, G. R. 1994. Hotspots of homologous recombination. Experientia. 50:234–241.

    Article  PubMed  CAS  Google Scholar 

  82. Stark, W. M., M. R. Boocock, and D. J. Sherratt. 1992. Catalysis by site-specific recombinases. Trends Genet. 8:432–439.

    Article  PubMed  CAS  Google Scholar 

  83. Theiss, P., A. Karpas, and K. S. Wise. 1996. Antigenic topology of the P29 surface lipoprotein of Mycoplasma fermentans: differential display of epitopes results in high-frequency phase variation. Infect. Immun. 64:1800–1809.

    PubMed Central  PubMed  CAS  Google Scholar 

  84. Theiss, P. and K. S. Wise. 1997. Localized frameshift mutation generates selective, high-frequency phase variation of a surface lipoprotein encoded by a mycoplasma ABC transporter operon. J. Bacteriol. 179:4013–4022.

    PubMed Central  PubMed  CAS  Google Scholar 

  85. Veal, A. D., H. W. Stokes and G. Daggard. 1992. Genetic exchange in natural microbial communities. P. 383–430. In K. C. Marshall, (ed.), Advances in Microbial Ecology. Vol. 12. Plenum Press, New York.

    Chapter  Google Scholar 

  86. Washburn, L. R., K. E. Weaver, E. J. Weaver, W. Donelan and S. Al-Sheboul 1998. Molecular characterization of Mycoplasma arthritidis variable surface protein MAA2. Infect. Immun. 66:2576–2586.

    PubMed Central  PubMed  CAS  Google Scholar 

  87. Washburn, L. R., E. J. Miller, and K. E. Weaver. 2000. Molecular characterization of Mycoplasma arthritidis membrane lipoprotein MAA1. Infect. Immun. 68:437–442.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  88. Watson, H. L., K. Dybvig, D. K. Blalock, and G. H. Cassell. 1989. Subunit structure of the variable V-l antigen of Mycoplasma pulmonis. Infect. Immun. 57:1684–1690.

    PubMed Central  PubMed  CAS  Google Scholar 

  89. Wise, K. S., D. Yogev, and R. Rosengarten. 1992. Antigenic Variation, p. 473–489. In J. Maniloff, R. N. McElhaney, L. R. Finch and J. B. Baseman (ed.), Mycoplasmas: Molecular Biology and Pathogenesis. American Society for Microbiology, Washington, D.C.

    Google Scholar 

  90. Wise, K. S. 1993. Adaptive surface variation in mycoplasmas. Trends Microbiol. 1:59–63.

    Article  PubMed  CAS  Google Scholar 

  91. Yogev, D., R. Rosengarten, R. Watson-McKown, and K. S. Wise. 1991. Molecular basis of Mycoplasma surface antigenic variation: a novel set of divergent genes undergo spontaneous mutation of periodic coding regions and regulatory sequences. EMBO J. 10:4069–4079.

    PubMed Central  PubMed  CAS  Google Scholar 

  92. Yogev, D., Watson-McKown , R. Rosengarten, J. Im, and K. S. Wise. 1995. Increased structural and combinatorial diversity in an extended family of genes encoding Vlp surface proteins of Mycoplasma hyorhinis. J. Bacteriol. 177: 5636–5643.

    PubMed Central  PubMed  CAS  Google Scholar 

  93. Zhang, Q. and K. S. Wise. 1996. Molecular basis of size and antigenic variation of a Mycoplasma hominis adhesin encoded by divergent vaa genes. Infect. Immun. 64:2737–2744.

    PubMed Central  PubMed  CAS  Google Scholar 

  94. Zhang, Q. and K. S. Wise. 1997. Localized reversible frameshift mutation in an adhesin gene confers a phase-variable adherence phenotype in mycoplasma. Mol. Microbiol. 25:859–869.

    Article  PubMed  CAS  Google Scholar 

  95. Zhang, Q. and K. S. Wise. 2001. Coupled phase-variable expression and epitope masking of selective surface lipoproteins increases surface phenotypic diversity in Mycoplasma hominis. Infect. Immun. 69:5177–5181.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Membrane and Ultrastructure Research, The Hebrew University-Hadassah Medical School, Jerusalem, Israel

    David Yogev

  2. School of Veterinary Science, The University of Melbourne, Australia

    Glenn F. Browning

  3. Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia, Missouri, USA

    Kim S. Wise

Authors
  1. David Yogev
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  2. Glenn F. Browning
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  3. Kim S. Wise
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Editor information

Editors and Affiliations

  1. The Hebrew University-Hadassah Medical School, Jerusalem, Israel

    Shmuel Razin

  2. University of Heidelberg, Heidelberg, Germany

    Richard Herrmann

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© 2002 Springer Science+Business Media New York

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Yogev, D., Browning, G.F., Wise, K.S. (2002). Genetic Mechanisms of Surface Variation. In: Razin, S., Herrmann, R. (eds) Molecular Biology and Pathogenicity of Mycoplasmas. Springer, Boston, MA. https://doi.org/10.1007/0-306-47606-1_19

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  • DOI: https://doi.org/10.1007/0-306-47606-1_19

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-8232-5

  • Online ISBN: 978-0-306-47606-8

  • eBook Packages: Springer Book Archive

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