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Experimental Design for Gene Microarray Experiments and Differential Expression Analysis

  • Chapter
Methods of Microarray Data Analysis II

Abstract

Advances in microarray technology open new challenges in data collection, analysis and interpretation. In this review paper, we focus on issues related to obtaining trustworthy normalized data and results of differential expression analysis. In particular, we briefly summarize discussions on sources of biological and technological variation, experimental design, design of arrays, normalization and error models, differential expression and multiple comparison issues. These issues remain major bottlenecks to developing standards and obtaining useful and applicable results of future analysis such as cluster analysis, network modeling, etc.

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Authors and Affiliations

  1. RTI International, USA

    G. V. Bobashev, S. Das & A. Das

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  1. G. V. Bobashev
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  2. S. Das
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  3. A. Das
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Editors and Affiliations

  1. Duke University Medical Center, USA

    Simon M. Lin  & Kimberly F. Johnson  & 

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© 2002 Kluwer Academic Publishers

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Bobashev, G.V., Das, S., Das, A. (2002). Experimental Design for Gene Microarray Experiments and Differential Expression Analysis. In: Lin, S.M., Johnson, K.F. (eds) Methods of Microarray Data Analysis II. Springer, Boston, MA. https://doi.org/10.1007/0-306-47598-7_3

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  • DOI: https://doi.org/10.1007/0-306-47598-7_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4020-7111-9

  • Online ISBN: 978-0-306-47598-6

  • eBook Packages: Springer Book Archive

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