Conclusion
In contrast with previous reports and columns we have a recovery of 100% vanadium from the Superose 12 column and a better resolution for proteins. So, we can already conclude that for gel filtration experiments with vanadium complexes this column is a good choice. For anion-exchange Chromatography, a Sepharose DEAE Fast Flow column gives also a recovery of 100% for vanadium, but the resolution for separating albumin and transferrin is not so good. Adapting column dimensions or gradient shape can therefore provide a solution. Out of the chromatographic results, we can conclude that vanadium in serum of rats, rabbits and humans principally is bound to transferrin.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Carmignani, M., Volpe, A.R., Sabbioni, E., Felaco, M., and Boscolo, P., 1998, Vanadium and the cardiovascular system; regulatory effects and toxicity, in: Vanadium in the Environment, Volume 2 (J.O. Nriagu, ed.), pp. 181–218, John Wiley and Sons, New York.
Chasteen, N.D., Lord, E.M., Thompson, H.J., and Grady, J.K., 1986, Vanadium complexes of transferrin and ferritin in the rat, Biochim. Biophys. Acta, 884:84–92.
Chatterjee, M. and Bishayee, A., 1998, Vanadium—A new tool for cancer prevention, in: Vanadium in the Environment, Volume 2 (J.O. Nriagu, ed.), pp. 347–390, John Wiley and Sons, New York.
Cornelis, R., Versieck, J., Mees, L., Hoste, J., and Barbier, F., 1981, The ultratrace element vanadium in human serum, Biol. Trace Elem. Res., 3:257–263.
De Cremer, K., De Kimpe, J., and Cornelis, R., 1999, Stability of vanadium(V)-protein complexes during chromatography, Fresenius J. Anal. Chem., 363:519–522.
Nielsen, F.H. and Uthus, E.O., 1990, The essentiality and metabolism of vanadium, in: Vanadium in Biological Systems, (N.D. Chasteen, ed.), pp. 51–62, Kluwer Academic Publishers, Dordrecht.
Pietra, R., Alimonti, A., Gallorini, M., Tanet, G., Caroli, S., and Sabbioni, E., 1991, Recent developments of pre-separation procedures for trace elements analysis of biological specimens, Acta Chim. Hung., 128:725–734.
Sabbioni, E. and Marafante, E., 1978, Metabolic patterns of vanadium in the rat, Bioinorg. Chem., 9:389–407.
Sabbioni, E., Marafante, E., Pietra, R., Goetz, L., Girardi, F., and Orvini, E., 1979, The association of vanadium with the iron transport system in human blood as determined by gel filtration and neutron activation analysis, in: Nuclear Activation Techniques in the Life Sciences 1978, proceedings of a symposium, (IEAE, ed.), pp. 179–192., IEAE, Vienna.
Sakurai, H. and Tsuji, A., 1998, Antidiabetic action of vanadium complexes in animals: blood glucose normalizing effect, organ distribution of vanadium, and mechanism for insulin-mimetic action, in: Vanadium in the Environment, Volume 2, (J.O. Nriagu, ed.), pp. 297–315, John Wiley and Sons, New York.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Kluwer Academic Publishers
About this chapter
Cite this chapter
De Cremer, K., Cornelis, K.R. (2002). Gelfiltration and Anion-Exchange Chromatography for the Separation of Vanadium Binding Proteins in Plasma of Rats, Rabbits, and Humans. In: Roussel, A.M., Anderson, R.A., Favier, A.E. (eds) Trace Elements in Man and Animals 10. Springer, New York, NY. https://doi.org/10.1007/0-306-47466-2_39
Download citation
DOI: https://doi.org/10.1007/0-306-47466-2_39
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-0-306-46378-5
Online ISBN: 978-0-306-47466-8
eBook Packages: Springer Book Archive