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Double-Quantum ESR and Distance Measurements

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Distance Measurements in Biological Systems by EPR

Part of the book series: Biological Magnetic Resonance ((BIMR,volume 19))

Abstract

“Allowed” double quantum ccherences (DQC) can now be routinely generated in disordered and oriented solids containing nitroxide biradicals and random distributions of stable radicals. The Pake doublets obtained from DQC pathways can be effectively used to determine a broad range of distances in the former case whereas decay constants yield concentrations in the latter. The DQC signals are strong and often comparable to standard single quantum signals. They are free of any large undesirable signals, so the DQ experiment is easy to perform. Their strong intensity permits the study of low concentrations of spins in samples typical of those ordinarily met in the case of doubly-labeled macromolecules such as proteins and polypeptides. The upper range of distances for systems labeled with nitroxides is estimated to be ca. 80 Å. In the limit of non-selective pulses the interpretation of DQC signals becomes independent of complicating geometric features which affect other ESR distance methods. The method is compared to other existing pulse distance measurement techniques and future improvements are also discussed.

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  1. Baker Laboratory of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, 14853, USA

    Petr. P. Borbat & Jack. H. Freed

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  1. Petr. P. Borbat
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  1. Ohio State University, Columbus, Ohio, USA

    Lawrence J. Berliner

  2. Department of Chemistry and Biochemistry, University of Denver, 80208, Denver, Colorado, USA

    Gareth R. Eaton  & Sandra S. Eaton  & 

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Borbat, P.P., Freed, J.H. (2002). Double-Quantum ESR and Distance Measurements. In: Berliner, L.J., Eaton, G.R., Eaton, S.S. (eds) Distance Measurements in Biological Systems by EPR. Biological Magnetic Resonance, vol 19. Springer, Boston, MA. https://doi.org/10.1007/0-306-47109-4_9

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  • DOI: https://doi.org/10.1007/0-306-47109-4_9

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