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Metabolic Flux and Subcellular Transport of Metabolites

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In Vivo Carbon-13 NMR

Part of the book series: Biological Magnetic Resonance ((BIMR,volume 15))

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Summary

Recent advances in 13C NMR observations of metabolism in intact tissues have taken the applications of this method from static measurements of relative metabolic activity to quantification of metabolic flux rate and metabolite transport. An exciting application from this new understanding comes from the translation of metabolic conditions between the cytosol and mitochondria for physiological studies of metabolic regulation.

The compartmentation of metabolic processes in living cells represents an important regulatory mechanism in coordinating energy production to cell functions. Metabolic communication between subcellular compartments is achieved largely by selective permeability of membranes to different metabolites, or carriermediated transport, which allows for efficient control of reaction pathways. Through the adjustment of metabolite distribution between the mitochondria and cytosol, the metabolic demands of overall physiological function by the cell can be translated to the energetic machinery of the mitochondria.

In this manner, the malate—aspartateshuttle interacts directly with the TCA cycle through carrier-mediated transport of α-ketoglutarate, malate, aspartate, and glutamate across the mitochondrial membrane. However, the regulatory role of the individual metabolite transporter on the mitochondrial membrane has only been studied in isolated mitochondria with an artificial cytosolic environment and certainly no physiological function. The dynamic-mode 13C NMR observations of intact tissues that have been discussed in this chapter can now contribute similar in-depth analysis of metabolic regulation in response to the physiological function of intact and in vivo organs.

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Authors and Affiliations

  1. NMR Center, Massachusetts General Hospital, Harvard Medical School, Bldg. 149, 13th Street, Charlestown, Massachusetts, 02129, USA

    E. Douglas Lewandowski

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  1. E. Douglas Lewandowski
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  1. Ohio State University, Columbus, Ohio, USA

    Lawrence J. Berliner  & Pierre-Marie Robitaille  & 

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© 2002 Kluwer Academic Publishers

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Lewandowski, E.D. (2002). Metabolic Flux and Subcellular Transport of Metabolites. In: Berliner, L.J., Robitaille, PM. (eds) In Vivo Carbon-13 NMR. Biological Magnetic Resonance, vol 15. Springer, Boston, MA. https://doi.org/10.1007/0-306-47078-0_4

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  • DOI: https://doi.org/10.1007/0-306-47078-0_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-45886-6

  • Online ISBN: 978-0-306-47078-3

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