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Frequency-Domain Fluorescence Spectroscopy

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Topics in Fluorescence Spectroscopy

Part of the book series: Topics in Fluorescence Spectroscopy ((TIFS,volume 1))

5.6. Summary

In this chapter we described instrumentation, basic theory, and applications of first- (1-200 MHz) and second-generation (2 GHz) frequency-domain fluorometers. The frequency-domain data provide excellent resolution of time-dependent spectral parameters. It is possible to resolve closely spaced fluorescence lifetimes, even in the picosecond region, and to determine multiexponential decays of anisotropy. Correlation times as short as 10 ps have been measured. Several novel applications are described, including picosecond fluorescence of hemoglobin and enhanced resolution of anisotropy decays using combination quenching and multiwavelength global measurements and analysis, as well as unusual behavior of phase and modulation data for associated anisotropy decays.

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  1. Department of Biological Chemistry, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Baltimore, Maryland, 21201, USA

    Joseph R. Lakowicz & Ignacy Gryczynski

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  1. Joseph R. Lakowicz
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  1. Center for Fluorescence Spectroscopy Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore, Maryland, USA

    Joseph R. Lakowicz

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© 2002 Kluwer Academic Publishers

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Lakowicz, J.R., Gryczynski, I. (2002). Frequency-Domain Fluorescence Spectroscopy. In: Lakowicz, J.R. (eds) Topics in Fluorescence Spectroscopy. Topics in Fluorescence Spectroscopy, vol 1. Springer, Boston, MA. https://doi.org/10.1007/0-306-47057-8_5

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