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Hematopoietic Stem and Progenitor Cells

  • Chapter
Human Cell Culture

Part of the book series: Human Cell Culture ((HUCC,volume 4))

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Summary

Mature blood cells, most of which exhibit a limited lifespan in vivo, are continuously generated from hematopoietic stem and progenitor cells. Stem cells are very rare in adult BM, but they have enough proliferative capacity to overcome stress and disease, potentially over several lifetimes. Control of stem cell growth and differentiation is a subject of intense study, and is known to be influenced by growth factors, stromal cells, ECM, and other culture conditions. These cells can be obtained from a number of primary tissue sources, and various means of processing and purification have been developed. Stem and progenitor cells are assayed through in vivo and in vitro methods, including xenogeneic transplant models, CFU assays, flow cytometry, and LTC-IC assays. Current culture methods for stem and progenitor cells are generally based on one of two approaches; the use of CD34-enriched cells in low density static culture, or the use of high density accessory cell-containing cultures supported by continuous medium perfusion. Both approaches are feasible, and each has its advantages and disadvantages for different applications. Stem cell cultures have been used in clinical studies to generate cells for SCT following cancer chemotherapy, as well as in basic scientific studies designed to better understand the complex process of hematopoiesis and carcinogenesis. Based upon the continued effort directed in this field of research and development, further advances can be expected, with the potential for considerable impact on the state of scientific knowledge and clinical practice.

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  1. Oncosis, 6199 Cornerstone Ct., Suite 111, San Diego, CA, 92121

    Manfred R. Koller

  2. Department of Bioengineering, UCSD, 9500 Gilman Dr, La Jolla, CA, 92093-0412

    Bernhard 0. Palsson

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  1. Oncosis, San Diego, CA, USA

    Manfred R. Koller

  2. Department of Bioengineering, University of California, La Jolla, CA, USA

    Bernhard O. Palsson

  3. Institute of Urolog, UniversityCollegeLondon, London, UK

    John R. W. Masters

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Koller, M.R., Palsson, B.0. (2002). Hematopoietic Stem and Progenitor Cells. In: Koller, M.R., Palsson, B.O., Masters, J.R.W. (eds) Human Cell Culture. Human Cell Culture, vol 4. Springer, Dordrecht. https://doi.org/10.1007/0-306-46886-7_1

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  • DOI: https://doi.org/10.1007/0-306-46886-7_1

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