Abstract
Lysozyme derived from hen egg white stimulated immunoglobulin (Ig) production by human-human hybridoma, HB4C5 cells producing monoclonal IgM. IgM production by HB4C5 cells was enhanced more than 13-fold by lysozyme at 380 μg/ml in serum-free medium. The mode of actions of lysozyme as an Ig production stimulating factor (IPSF) was investigated. Lysozyme enhanced Ig production by transcription-suppressed HB4C5 cells. However, the enzyme was ineffective to accelerate IgM production by translation-suppressed HB4C5 cells. In addition, the intracellular IgM content of HB4C5 cells, which were suppressed post-transcription process, was obviously increased by the addition of lysozyme. These findings suggest that lysozyme accelerates translation process to enhance Ig productivity.
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References
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© 1999 Kluwer Academic Publishers
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Sugahara, T., Murakami, F., Sasaki, T. (1999). The Mode of Actions of Lysozyme as an Immunoglobul in Production Stimulating Factor (IPSF). In: Bernard, A., Griffiths, B., Noé, W., Wurm, F. (eds) Animal Cell Technology: Products from Cells, Cells as Products. Springer, Dordrecht. https://doi.org/10.1007/0-306-46875-1_8
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DOI: https://doi.org/10.1007/0-306-46875-1_8
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-6075-9
Online ISBN: 978-0-306-46875-9
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