Abstract
This paper describes the test of several new vegetarian hydrolysates (peptones of soy, rice, wheat gluten etc.) as protein-free medium supplements for the production of a recombinant therapeutic protein. Multiple peptone-supplemented, continuous perfusion bioreactor experiments were conducted. Cell specific rates and product quality studies were obtained for the various peptones and compared with peptone-free medium. It was found that peptones confer a nutritional benefit, especially at low dilution rates, for a recombinant BHK cell line used in this investigation. The specific productivity increased 20–30% compared to the peptone-free controls. However, this benefit was also fully delivered by using fortified medium in place of the peptone-enriched media.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Chuppa, S., Tsai, Y-S., Yoon, S., Shackleford, S., Rozales, C., Bhat, R., Tsay, G., Matanguihan, C., Konstantinov, K. and Naveh, D. (1997) Fermentor temperature as a tool tor control of high-density perfusion cultures of mammalian cells. Biotechnol. Bioeng. 55: 328–338
Dyring, C., Hansen, H. and Emborg, C. (1994) Observations on the influence of glutamine, asparagine and peptone on growth and t-PA productionof Chinese hamster ovary (CHO) cells. Cytotechnology 16: 37–42
Gu, X., Xie, L., Harmon, B. and Wang, D (1997) Influence of Primatone RL supplementation on sialylation of recombinant human produced by Chinese hamster ovary cell culture using serum-free media. Biotechnol. Bioeng. 56: 353–360
Jan, D., Jones, S. Emery, A. and AI-Rubeai, M. (1994) Peptone, a low cost growth-promoting nutrient for intensive animal cell culture. Ctytotechnology 16: 17–26
Nyberg, G., Balcarcel, R., Follstad, B., Stephanopoulos, G., Wang, D. (1999) Metabolism of peptide amino acids by Chines hamster ovary cells grown in a complex medium. Biontechnol. Bioeng. 62: 324–335
Keen, M. and Rapson, N. (1995) Development of a serum-free culture medium for the large scale production of recombinant protein from Chinese hamster ovary cell line. Cytotechnology 17: 153–163
Xie, L., Nyberg, G., Gu, X., Li, H., Möllbom, F and Wang, D. (1997) Gamma-Interferon production and quality in stoichiometric fed-batch cultures of Chinese hamster ovary (CHO) cells under serum-free conditions. Biotechnol. Bioeng. 56: 577–582
Zhang, Y., Zhou, Y. and Yu, J. (1994) Effects of peptone on hybridoma growth and monoclonal antibody formation. Cytotechnology 16: 147–150
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1999 Kluwer Academic Publishers
About this chapter
Cite this chapter
Heidemann, R. et al. (1999). The Use of Peptones as Medium Additives for High-Density Perfusion Cultures of Animal Cells. In: Bernard, A., Griffiths, B., Noé, W., Wurm, F. (eds) Animal Cell Technology: Products from Cells, Cells as Products. Springer, Dordrecht. https://doi.org/10.1007/0-306-46875-1_45
Download citation
DOI: https://doi.org/10.1007/0-306-46875-1_45
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-6075-9
Online ISBN: 978-0-306-46875-9
eBook Packages: Springer Book Archive