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Animal Cell Technology: Products from Cells, Cells as Products pp 131–133Cite as

A Model for the On-Line Scrutiny of Metabolism: Its Application to the Changing Nutritional Demands of Cultured Animal Cells

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Abstract

The development of a novel on-line metabolic probe, the combined heat flux biosensor, has facilitated the formation of a working metabolic model that can be integrated with an on-line strategy to feed nutrients. The methodology is based on the monotonic relationship between heat flux and the consumption flux of substrates. A theoretical demonstration of it is validated by data from continuous cultures on the relationship that is used to implement a feeding strategy in which heat flux is the control variable.

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References

  1. Guan, Y., Evans, P.M., and Kemp, R.B. (1998) Biotechnol. Bioeng. 58, 464–477.

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Authors and Affiliations

  1. Institute of Biological Sciences, University of Wales, Aberystwyth, SY23 3DA, UK

    Y.H. Guan & R.B. Kemp

Authors
  1. Y.H. Guan
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  2. R.B. Kemp
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Editor information

A. Bernard B. Griffiths W. Noé F. Wurm

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© 1999 Kluwer Academic Publishers

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Guan, Y., Kemp, R. (1999). A Model for the On-Line Scrutiny of Metabolism: Its Application to the Changing Nutritional Demands of Cultured Animal Cells. In: Bernard, A., Griffiths, B., Noé, W., Wurm, F. (eds) Animal Cell Technology: Products from Cells, Cells as Products. Springer, Dordrecht. https://doi.org/10.1007/0-306-46875-1_29

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  • DOI: https://doi.org/10.1007/0-306-46875-1_29

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-6075-9

  • Online ISBN: 978-0-306-46875-9

  • eBook Packages: Springer Book Archive

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