Summary
We obtained 2,202 multi-clone immortalized human B-cell library stocks from seven healthy donors by transforming B-cells with the Epstein-Barr virus. The B-cell library contained various kinds of B-lymphobiastoid cells secreting antibodies to rice, soybeans, milk, and eggs. Finally, we obtained human-mouse hybridomas secreting antibodies to rice allergens. We concluded that the method developed here would be a powerful tool for analyzing food allergens.
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© 2002 Kluwer Academic Publishers
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Shinmoto, H., Nakahara, K., Ichikawa, R., Korori, M., Tsushida, T. (2002). Establishment of Human-Mouse Hybridomas Secreting Antibodies to Rice-Allergens. In: Ikura, K., Nagao, M., Masuda, S., Sasaki, R. (eds) Animal Cell Technology: Challenges for the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/0-306-46869-7_30
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DOI: https://doi.org/10.1007/0-306-46869-7_30
Publisher Name: Springer, Dordrecht
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