Abstract
Growth profiles of the batch and fed-batch culture of hybridoma cells producing monoclonal antibody were simulated using a structured model previously developed. The model describes the production of cellular macromolecules and monoclonal antibody, the metabolism of glucose and glutamine with the production of lactate and ammonia, and the profiles of cell growth in batch and fed-batch culture. Equations describing the cells arrested in G1 phase (Linardos et al., 1992; Suzuki et al., 1989) was included in this model to describe the increase of the specific antibody productivity in the near-zero specific growth rate, which was observed in the recent experiments in fed-batch cultures of a previous study. This model well predicted the increase ofspecific antibody production rate and the decline of the specific production rate of cellular macromolecules such as DNA, RNA, protein, and polysaccharide with lower specific growth rate in the fed-batch culture.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Garatun-Tjeldstø O., Pryme, I.F., Weltman. J.K., and Dowben, R.M. (1976) Synthesis and secretion oflight-chain immunoglobulin in two successive cycles of synchronized plasmacytoma cells. J. Cell. Biol 68. 232–239.
Linardos. T.I., Kalogerakis, N., and Behie. LA (1992) Kinetic analysis of hybridoma growth and monoclonal antibody production in semi-continuous culture. Biotech Bioeng 40, 359–368.
Sanderson. C.S. (1996) The development and application of a structured model for animal cell metabolism. Ph.D. Thesis. University of Sydney.
Sanderson. C.S., Barton, G.W., and Barford. J.P (1995) Optimization of animal cell culture media using dynamic simulation. Computers Chem, Eng, 19, s681–s686
Suzuki. E. and Ollis. D.F., 1989 Cell cycle model for antibody production kinetics. Biotech. Bioeng. 34, 1398–1402.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 2002 Kluwer Academic Publishers
About this chapter
Cite this chapter
Jang, J.D., Barford, J. (2002). A Kinetic Model of Macromolecular Metabolism in Batch and Fed-Batch Cultures of Hybridoma Cells Producing Monoclonal Antibody. In: Ikura, K., Nagao, M., Masuda, S., Sasaki, R. (eds) Animal Cell Technology: Challenges for the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/0-306-46869-7_13
Download citation
DOI: https://doi.org/10.1007/0-306-46869-7_13
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-5805-3
Online ISBN: 978-0-306-46869-8
eBook Packages: Springer Book Archive