Abstract
The effects of phosphatidic acid (PA) and lysophosphatidic acid (LPA) on the growth of Chinese hamster ovary (CHO) cells in serum-free media were evaluated by MTT assay. Supplementing PA or LPA to a protein-free basal synthetic medium or a serum-free medium containing insulin and transferrin markedly stimulated the growth of CHO cells, suggesting that PA and LPA are promising growth-promoting supplements for use in constituting a low-protein serum-free medium.
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References
Mizuguchi, M., Matsumoto, K., and Onodera, K. (1993) Production of the human growth hormone in serum-free UC203 medium, in S. Kaminogawa et al. (eds.) Animal Cell Technology: Basic & Applied Aspects 5, Kluwer Academic Publishers, Dordrecht, pp. 57–61.
Moolenaar, W.H. (1994) Lysophosphatidic acid, a multifunctional phospholipid messenger, J. Biol. Chem. 270, 12949–12952.
Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol. Methods 65, 55–63.
Murakami, H., Masui, H., Sato, G.H., Sueoka. N., Chow, T.P., and Kano-Sueoka, T. (1982) Growth of hybridoma cells in serum-free medium: ethanolamine is an essential component, Proc. Natl. Acad. Sci. USA 79, 1158–1162.
Urlaub, G. and Chasin L.A. (1980) Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity, Proc. Natl. Acad. Sci. USA 77, 4216–4220.
van Corven, E.J., Groenink, A., Jalink, K., Eichholtz, T., Moolenaar, W.H. (1989) Lysophosphatidate-induced cell proliferation: identification and dissection of signaling pathways mediated by G proteins, Cell 59, 45–54.
Yamaji, H. and Fukuda, H. (1992) Growth and death behaviour of anchorage-independent animal cells immobilized within porous support matrices, Appl. Microbiol. Biotechnol. 37, 244–251.
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© 2002 Kluwer Academic Publishers
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Sakai, K., Matsunaga, T., Yamaji, H., Fukuda, H. (2002). Growth Stimulation of CHO Cells by Phospholipids in Serum-Free Culture. In: Ikura, K., Nagao, M., Masuda, S., Sasaki, R. (eds) Animal Cell Technology: Challenges for the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/0-306-46869-7_12
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DOI: https://doi.org/10.1007/0-306-46869-7_12
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