Skip to main content
SpringerLink
Log in

Formation of Cylindrical Multicellular Aggregate (Cylindroid) of Rat Hepatocytes on Pressed Sheet of Polyurethane Foam

  • Chapter
Animal Cell Technology: Basic & Applied Aspects

  • 255 Accesses

Abstract

Primary rat hepatocytes formed cylindrical multicellular aggregates on a pressed sheet of polyurethane foam (pressed-PUF) as a culture substratum. The sheet was made up by pressing PUF during the process of foaming. The diameter and length of the hepatocyte cylindroid is approximately 200 ~ 500 μm and 500 μm ~ 2 mm, respectively. Its activities of liver specific functions (albumin secretion and ammonia metabolism) were equal to those of hepatocyte spheroids. These results indicated that hepatocytes cylindroid are a kind of organoid, and will provide a useful culture technique for high cell density culture.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Reference

  1. Crane LJ, Miller DL (1977) Plasma protein synthesis by isolated rat hepatocytes. J. Cell. Biol. 72: 11–25.

    Article  CAS  PubMed  Google Scholar 

  2. Koide N, Sakaguchi K, Koide Y, Asano K, Kawaguchi M, Matsushima H, Takenami T, Shinji T, Mod M and Tsuji T (1990) Exp. Cell Res. 186: 227–235.

    Article  CAS  PubMed  Google Scholar 

  3. Langer R, Vacanti JP (1993) Tissue Engineering. Science 260: 920–926

    CAS  PubMed  Google Scholar 

  4. Matsushita T, Ijima H, Koide N and Funatsu K (1991) High albumin production by multicellular spheroids of adult rat hepatocytes formed in the pores of polyurethane foam. Appl. Microbiol. Biotechnol. 36: 324–326.

    Article  CAS  PubMed  Google Scholar 

  5. Matsushita T, Ketayama M, Kamihara K and Funatsu K (1990) Anchorage-dependent mammalian cell culture using polyurethane foam as a new substratum for cell attachment. Appl. Microbiol. Biotechnol. 33: 287–290.

    Article  CAS  PubMed  Google Scholar 

  6. Wezel AL van (1967) Growth of cell-strains and primary cells on micro-carriers in homogeneous culture. Nature 216: 64–65.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-81, Japan

    Hiroshi Mizumoto, Mitsuru Kaneko, Kohji Nakazawa, Hiroyuki Ijima, Taku Matsushita & Kazumori Funatsu

Authors
  1. Hiroshi Mizumoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mitsuru Kaneko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kohji Nakazawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hiroyuki Ijima
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Taku Matsushita
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kazumori Funatsu
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Bioscience Center, Nagoya University, Nagoya, Japan

    Y. Kitagawa

  2. Department of Applied Biological Sciences, Graduate School of Agricultural Sciences, Nagoya University, Nagoya, Japan

    T. Matsuda

  3. Department of Biotechnology, Graduate School of Engineering, Nagoya University, Nagoya, Japan

    S. Iijima

Rights and permissions

Reprints and permissions

Copyright information

© 1999 Kluwer Academic Publishers

About this chapter

Cite this chapter

Mizumoto, H., Kaneko, M., Nakazawa, K., Ijima, H., Matsushita, T., Funatsu, K. (1999). Formation of Cylindrical Multicellular Aggregate (Cylindroid) of Rat Hepatocytes on Pressed Sheet of Polyurethane Foam. In: Kitagawa, Y., Matsuda, T., Iijima, S. (eds) Animal Cell Technology: Basic & Applied Aspects., vol 1. Springer, Dordrecht. https://doi.org/10.1007/0-306-46865-4_39

Download citation

  • DOI: https://doi.org/10.1007/0-306-46865-4_39

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-5451-2

  • Online ISBN: 978-0-306-46865-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation