Establishment of an Apoptosis-Resistant and Growth-Controllable Cell Line by Transfecting With Inducible Antisense c-Jun Gene

Abstract

By treating the c-jun AS cells with dexamethasone (DEX) in DMEM supplemented with 10% serum, the growth of the cells were completely suppressed for duration of 16 days with high cell viability of above 86%. The c-jun AS cells were able to grow well to 10⁶ cells/ml without supplementation of any serum components, keeping viability high above 80% for 8 days when cultured in the absence of DEX. The c-jun AS cells stayed at a constant cell density and high viability above 80% for 8 days when they were cultured in the presence of DEX under serum-deprivation. In contrast to the c-jun AS cells, the wild type F-MEL cells were unable to grow and they died by apoptosis in 3 days under the serum-deprivation; the internucleosomal cleavage of DNA, a landmark of apoptosis, was clearly detectable. Thus the c-jun AS cell line that is resistant to apoptosis induced by serum-deprivation and can reversibly and viably be growth-arrested was established.