Abstract
We have produced a recombinant C-terminal α-amidating enzyme (799Bg/IIα-AE) derived from Xenopus laevis by culturing a CHO cell line named 3μRecently, we demonstrated that culturing μ-1S cells at a temperature below 37°C led to the following phenomena: inhibited cell growth with high viability, enhanced cellular productivity (maximally at 32°C), and suppressed medium consumption and release of impurities from the cells. Therefore, it is suggested that the 799Bg/IIα-AE production will be increased by culturing a sufficient number of the cells at a low temperature (especially at 32°C). Application of the low temperature culture to batch and perfusion cultures was effective in comparison with the culture at 37°C: the productivity per medium and the productivity per time were increased several fold with enhanced cellular productivity at a low culture temperature. The low temperature culture also increased the relative content of 799Bg/IIα-AE in the supernatant and reduced the glucose consumption.
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© 1999 Kluwer Academic Publishers
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Furukawa, K., Ohsuye, K. (1999). Enhancement of Productivity of Recombinant α-Amidating Enzyme by Low Temperature Culture. In: Kitagawa, Y., Matsuda, T., Iijima, S. (eds) Animal Cell Technology: Basic & Applied Aspects., vol 1. Springer, Dordrecht. https://doi.org/10.1007/0-306-46865-4_27
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DOI: https://doi.org/10.1007/0-306-46865-4_27
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-5451-2
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