Abstract
In order to inhibit the CHO CNAH translation, responsible for the conversion of NeuAc to NeuGc residues, fragments of different sizes from the mouse cnah cDNA have been cloned as antisense fragments. It appeared that the different antisense species tested were able to inhibit the CNAH translation in vitro. However, when decreasing the amount of the antisense fragments by half, different efficiencies were found according to the fragment’s length or its sequence.
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© 1998 Kluwer Academic Publishers
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GGregoire, A., Visvikis, A., Marc, A., Goergen, J.L. (1998). Antisense RNA for the Elimination of Neugc Rresidues from Recombinant Glycoproteins. In: Merten, OW., Perrin, P., Griffiths, B. (eds) New Developments and New Applications in Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/0-306-46860-3_34
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DOI: https://doi.org/10.1007/0-306-46860-3_34
Publisher Name: Springer, Dordrecht
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