Summary
Two different genetic modifications of hybridoma cells are studied. First, cells are transfected with the glutamine synthetase gene, and important modifications in their physiology can be observed, that are reflected on the corresponding analysis of the intracellular metabolic fluxes. For the modified cell line the need for glutamine feeding in the culture has been eliminated and the ammonium production suppressed. Moreover, the glucose uptake rate has been reduced to half with respect to the parental strain, while maintaining a similar growth pattern. Second, antisense RNA techniques were employed to inhibit partially the expression of two glycolytic enzymes in order to create some rate limiting steps in the glycolytic pathway. The preliminary results obtained with the glucose transporter and enolase genes show the potential of such approach.
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© 1998 Kluwer Academic Publishers
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Cairó, J.J., Paredes, C., Gòdia, F., Prats, E., Azorín, F., Cornudella, L. (1998). Modification of Hybridoma Cells Metabolism. In: Merten, OW., Perrin, P., Griffiths, B. (eds) New Developments and New Applications in Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/0-306-46860-3_30
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DOI: https://doi.org/10.1007/0-306-46860-3_30
Publisher Name: Springer, Dordrecht
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