Conclusion
Histidine tagged recombinant Dm-dNK was shown to be superior to untagged protein as antigen for generating antibodies against Dm-dNK. The antiserum to rDm-dNK-8His was able to recognise recombinant Dm-dNK expressed in different hosts and will therefore be a valuable tool to investigate subcellular localization and expression levels of Dm-dNK in homologous and heterologous expression.
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Knecht, W., Munch-Petersen, B., Piskur, J. (2002). Polyclonal Antibodies Against the Ultrafast Multisubstrate Deoxyribonucleoside Kinase from Drosophila Melanogaster. In: Zoref-Shani, E., Sperling, O. (eds) Purine and Pyrimidine Metabolism in Man X. Advances in Experimental Medicine and Biology, vol 486. Springer, Boston, MA. https://doi.org/10.1007/0-306-46843-3_51
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DOI: https://doi.org/10.1007/0-306-46843-3_51
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