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Assessment of Chromogranin a Using Two-Site Immunoassay

Selection of a Monoclonal Antibody Pair Unaffected by Human Chromogranin A Processing

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Chromogranins

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 482))

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References

  • Abrahamsson, P.A., Cockett, A.T.K., and di Sant’Agnese, P.A., 1998, Prognostic significance of neuroendocrine differentiation inclinically localized prostatic carcinoma. Prostate Suppl. 8: 37–42.

    Article  PubMed  CAS  Google Scholar 

  • Baudin, E., Gigliotti, A., Ducreux, R., Ropers, J., Comoy, E., Sabourin, J.C., Bidart, J.M., Cailleux, A.F., Bonacci, R., Ruffie, P., and Schlumberger, M., 1998, Neuron-specific enolase and chromogranin A as markers of neuroendocrine tumours. Br. J. Cancer 78: 1102–1107.

    PubMed  CAS  Google Scholar 

  • Blennow, K., Davidsson, P., Wallin, A., and Ekman, R., 1995, Chromogranin A in cerebrospinal fluid: a biochemical marker for synaptic degeneration in Alzheimer’s disease? Dementia 6: 306–311.

    Article  PubMed  CAS  Google Scholar 

  • Blumenfeld, W., Chandhoke, D.K., Sagerman, P., and Turi, G.K., 1996, Neuroendocrine differentiation in gastric adenocarcinomas — an immunohistochemical study. Arch. Pathol. Lab. Med. 120: 478–481.

    PubMed  CAS  Google Scholar 

  • Bonkhoff, H., 1998, Neuroendocrine cells in benign and malignant prostate tissue: morphogenesis, proliferation and androgen receptor status. Prostate Suppl. 8: 8–12.

    Google Scholar 

  • Boomsma, F., Bhaggoe, U.M., Man in’ t Veld, A.J., and Schalekamp, M.A., 1995, Sensitivity and specificity of a new ELISA method for determination of chromogranin A in the diagnosis of pheochromocytoma and neuroblastoma. Clin. Chim. Acta 239: 57–63.

    Article  PubMed  CAS  Google Scholar 

  • Cussenot, O., Villette, J.M., Valeri, A., Cariou, G., Desgrandchamps, F., Cortesse, A., Meria, P., Teillac, P., Fiet, J., and Leduc, A., 1996, Plasma neuroendocrine markers in patients with benign prostatic hyperplasia and prostatic carcinoma. J. Urol. 155: 1340–1343.

    Article  PubMed  CAS  Google Scholar 

  • Deftos, L.J., 1998, Granin-A, parathyroid hormone-related protein, and calcitonin gene products in neuroendocrine prostate cancer. Prostate Suppl. 8: 23–31.

    Article  PubMed  CAS  Google Scholar 

  • Deftos, L.J., and Abrahamsson, P.A., 1998, Granins and prostate cancer. Urology 51: 141–145.

    Article  PubMed  CAS  Google Scholar 

  • Deftos, L.J., Nakada, S., Burton, D.W., Di Sant’Agnese, P., Cockett, T.K, and Abrahamsson, P.A., 1996, Immunoassay and immunohistology studies of chromogranin A as a neuroendocrine marker in patients with carcinoma of the prostate. Urology 48: 58–62.

    Article  PubMed  CAS  Google Scholar 

  • Degorce, F., Goumon, Y., Jacquemart, L., Vidaud, C., Bellanger, L., Pons-Anicet, D., Seguin, P., Metz-Boutigue, M.H., and Aunis, D., 1999, A new human chromogranin A (CGA) immunoradiometric assay involving monoclonal antibodies raised against the unprocessed central domain. Br. J. Cancer 79: 65–71.

    Article  PubMed  CAS  Google Scholar 

  • Di Sant’Agnese, P.A., 1992, Neuroendocrine differentiation in carcinoma of the prostate. Diagnostic, prognostic, and therapeutic implications. Cancer 70: 254–268.

    Article  Google Scholar 

  • Gadroy, P., Stridsberg, M., Capon, C., Michalski, J.C., Strub, J.M., Van Dorsselaer, A., Aunis, D., and Metz-Boutigue, M.H., 1998, Phosphorylation and 0-glycosylation sites of human chromogranin A (CGA79-439) from urine of patients with carcinoid tumors. J. Biol. Chem. 273: 34087–34097.

    Article  PubMed  CAS  Google Scholar 

  • Hsiao, R.J, Seeger, R.C., Yu, A.L., and OConnor, D.T., 1990, Chromogranin A in children with neuroblastoma. J. Clin. Invest. 85: 1555–1559.

    Article  PubMed  CAS  Google Scholar 

  • Hsiao, R.J., Parmer, R.J., Takiyyuddin, M.A., and OConnor, D.T., 1991, Chromogranin A storage and secretion: sensitivity and specificity for the diagnosis of pheochromocytoma. Medicine 70: 33–45.

    Article  PubMed  CAS  Google Scholar 

  • Kadmon, D., Thompson, T.C., Lynch, G.R., and Scardino, P.T., 1991, Elevated plasma chromogranin A concentrations in prostatic carcinoma. J. Urol. 146: 358–361

    PubMed  CAS  Google Scholar 

  • Lugardon, K., Raffner, R., Goumon, Y., Corti, A., Delmas, A., Bulet, P., Aunis, D., and Metz-Boutigue, M.H., 2000, Antibacterial and antifungal activities of vasostatin-1, the N-terminal fragment ofchromogranin A. J. Biol. Chem. 275: in press.

    Google Scholar 

  • Metz-Boutigue, M.H., Garcia-Sablone, P., Hogue-Angeletti, R., and Aunis, D., 1993, Intracellular and extracellular processing of chromogranin-A. Determination of cleavage sites. Eur. J. Biochem. 217: 247–257.

    Article  PubMed  CAS  Google Scholar 

  • Muiioz, D.G., 1991, Chromogranin A-like immunoreactive neurites are major constituents of senile plaques. Lab. Invest. 64: 826–32.

    Google Scholar 

  • Nishimura, M., Tomimoto, H., Suenaga, T., Nakamura, S., Namba, Y., Ikeda, K., Akiguchi, I., and Kimura, J., 1994, Synaptophysin and chromogranin A immunoreactivities of Lewy bodies in Parkinson’s disease brains. Brain Res. 634: 339–344.

    Article  PubMed  CAS  Google Scholar 

  • Nobels, F.R.E., Kwekkeboom, D.J., Coopmans, W., Schoenmakers, H.H., Lindemans, J., De Herder, W.W., Krenning, P., Bouillon, R., and Lamberts, S.W.J., 1997, Chromogranin A as serum marker for neuroendocrine neoplasia: comparison with neuron-specific enolase and the a-subunit of glycoprotein hormones. J. Clin. Endocrinol. Metabol. 82: 2622–2628.

    Article  CAS  Google Scholar 

  • O’Connor, D.T., and Bernstein, K.N., 1984, Radioimmunoassay of chromogranin A in plasma as a measure of exocytotic sympathoadrenal activity in normal subjects and patients with pheochromocytoma. New Engl. J. Med. 311: 764–770.

    Google Scholar 

  • O’Connor, D.T., and Deftos, L.J., 1986, Secretion of chromogranin A by peptide-producing endocrine neoplasms. New Engl. J. Med. 314: 1145–1151.

    Google Scholar 

  • OConnor, D.T., Pandian, M.R., Carlton, E., Cervanka, J.H., and Hsiao, R.J., 1989, Rapid radioimmunoassay of circulating chromogranin A: in vitro stability, exploration of the neuroendocrine character of neoplasia, and assessment of the effects of organ failure. Clin. Chem. 35: 1631–1637.

    CAS  Google Scholar 

  • OConnor, D.T., Kailasam, M.T., Thal, L.J., 1993, Cerebrospinal fluid chromogranin A is unchanged in Alzheimer dementia. Neurobiol. Aging 14: 267–269.

    Article  CAS  Google Scholar 

  • Pagani, A., Papotti, M., Abbona, G.C., and Bussolati, G., 1995, Chromogranin gene expressions in colorectal adenocarcinomas. Modern Pathol. 8: 626–632.

    CAS  Google Scholar 

  • Portela-Gomes, G.M., Stridsberg, M., Johansson, H., and Grimelius, L., 1997, Complex colocalization of chromogranins and neurohormones in the human gastrointestinal tract J. Histochem. Cytochem. 45: 815–822.

    PubMed  CAS  Google Scholar 

  • Ravery, V., Meulemans, A., de La Taille, A., Toublanc, M., and Boccon-Gibod, D., 1999, Etude de la chromogranine A tissulaire et sérique dans I’hypertrophie béigne de la prostate et le cancer prosatique. Immunoanal. Biol. Spéc. 14: 113–118.

    Article  Google Scholar 

  • Simon, J. P., and Aunis, D., 1989, Biochemistry of the chromogranin A protein family, Biochem.J. 262: 1–13.

    PubMed  CAS  Google Scholar 

  • Stridsberg, M., CØberg, K., Li, Q., Engstrom, U., and Lundqvist, G., 1995, Measurements of chromogranin A, chromogranin B (secretogranin I), chromogranin C (secretogranin II) and pancreastatin in plasma and urine from patients with carcinoid tumours and endocrine pancreatic tumours. J. Endocrinol. 144: 49–59.

    Article  PubMed  CAS  Google Scholar 

  • Syversen, U., Jacobsen, M.B., O’Connor, D.T., Ronning, K., and Waldum, H.L., 1994, Immunoassays for measurement of chromogranin A and pancreastatin-like immunoreactivity in humans: correspondence in patients with neuroendocrine neoplasia. Neuropeptides 26: 201–206.

    Article  PubMed  CAS  Google Scholar 

  • Taupenot, L., Remacle, J.E., Helle, K.B., Aunis, D., and Bader, M.F., 1995, Recombinant human chromogranin A: expression, purification and characterization of the N-terminal derived peptides. Regul. Pept. 56: 71–88.

    Article  PubMed  CAS  Google Scholar 

  • Wassberg, E., Stridsberg, M., and Christofferson, R., 1996, Plasma levels of chromogranin A are directly proportional to tumour burden in neuroblastoma. J. Endocrinol. 151: 225–230.

    Article  PubMed  CAS  Google Scholar 

  • Weiler, R., Fischer-Colbrie, R., Schmid, K. W., Feichtinger, H., Bussolati, G., Grimelius, L., Krisch, K., O’Connor, D.T., and Winkler, H., 1988, Immunological studies on the occurrence and properties of chromogranin A and B and secretogranin II in endocrine tumors. Am. J. Surg. Pathol. 12: 877–884.

    Article  PubMed  CAS  Google Scholar 

  • Wilson, B.S., and Lloyd, R.V., 1984, Detection of chromogranin in neuroendocrine cells with a monoclonal antibody. Am. J. Pathol. 115: 458–468.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. CIS Biointernational, Division In Vitro Technologies, Bagnols-sur-Cèze, France

    François Degorce

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  1. François Degorce
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Editor information

Editors and Affiliations

  1. University of Bergen, Bergen, Norway

    Karen B. Helle

  2. INSERM U338, Centre of Neurochemistry, Strasbourg, France

    Dominique Aunis

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© 2002 Kluwer Academic Publishers

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Degorce, F. (2002). Assessment of Chromogranin a Using Two-Site Immunoassay. In: Helle, K.B., Aunis, D. (eds) Chromogranins. Advances in Experimental Medicine and Biology, vol 482. Springer, Boston, MA. https://doi.org/10.1007/0-306-46837-9_27

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  • DOI: https://doi.org/10.1007/0-306-46837-9_27

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-46446-1

  • Online ISBN: 978-0-306-46837-7

  • eBook Packages: Springer Book Archive

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