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Antibacterial and Antifungal Peptides Derived from Chromogranins and Proenkephalin-A

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Chromogranins

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References

  • Aardal, S., and Helle, K.B., 1992, The vasoinhibitory activity of bovine chromogranin A fragment (vasostatin) and its dependence of extracellular calcium in isolated segments of human blood vessels. Regul. Pept. 41: 9–18.

    Article  PubMed  CAS  Google Scholar 

  • Aardal, S., Helle, K.B., Elsayed, S., Reed, R.K., and Serck-Hanssen, G., 1993, Vasostatins, comprising the N-terminal domain of chromogranin A, suppress tension in isolated human blood vessel segments. J. Neuroendocrinol. 5: 405–412.

    PubMed  CAS  Google Scholar 

  • Bevins, C.L., 1994, Antimicrobial peptides as agents of mucosal immunity. In: Antimicrobial peptides, (J. Marsh and J.A. Goode, eds.), John Urley and Sons, Chichester, pp. 250–258.

    Google Scholar 

  • Boman, H., 1995, Peptide antibiotics and their role in innate immunity. Annu. Rev. Immunol. 13: 61–92.

    Article  PubMed  CAS  Google Scholar 

  • Charlet, M., Chemysh, S., Philippe, H., Hetru, C., Hoffmann, J.A., and Bulet, P., 1996, Innate immunity. Isolation of several cysteine-rich antimicrobial peptides from the blood of a mollusc, Mytilus edulis. J. Biol. Chem. 271: 21808–21813.

    PubMed  CAS  Google Scholar 

  • Ciesielski-Treska, J., Ulrich, G., Taupenot, L., Chasserot-Golaz, S., Corti, A., Aunis, D. and Bader, M.F., 1998, Chromogranin A induces a neurotoxic phenotype in brain microglial cells. J. Biol. Chem. 273: 14339–14346.

    Article  PubMed  CAS  Google Scholar 

  • Corti, A., Perez Sanchez, L., Gaspam, A., Curnis, F., Longhi, R., Brandazza, A., Siccardi, A.G., and Sidoli, A., 1997, Production and structure characterization of recombinant chromogranin A N-terminal fragments (vasostatins). Evidence of dimer-monomer equilibrium. Eur. J. Biochem. 248: 692–699.

    Article  PubMed  CAS  Google Scholar 

  • Diamond, G., Russel, J.P. and Bevins, C.L., 1996, Inducible expression of an antibiotic peptide gene in lipopolysaccharide-challenged tracheal epithelial cells. Proc. Natl. Acad. Sci. USA 93: 5156–5160.

    Article  PubMed  CAS  Google Scholar 

  • Dillen, L., Miserez, B., Claeys, M., Aunis, D., and De Potter, W., 1993, Post-translational processing of of proenkephalin-A and chromogranins/secretogranins. Neurochem. Int. 22: 315–352.

    Article  PubMed  CAS  Google Scholar 

  • Evans, E.W., Beach, G.G., Wunderlich, J., and Harmong, B.G., 1994, Isolation of antimicrobial peptides from avian heterophiles. J. Leukoc. Biol. 56: 661–665.

    PubMed  CAS  Google Scholar 

  • Gaspam, A., Sidoli, A., Perez Sanchez, L., Longhi, R., Siccardi, A.G., Marchisio, P.C., and Corti, A., 1997, Chromogranin A fragments modulate cell adhesion. Identification and characterization ofa pro-adhesive domain. J. Biol. Chem. 272: 20835–20843.

    Google Scholar 

  • Gennaro, R., Romeo, D., Skerlavaj, B., and Zanetti, M., 1991, In: Blood Cell Biochemistry vol. 3, (J.R. Hams, ed.), Plenum Press, New-York, pp. 335–368.

    Google Scholar 

  • Goumon, Y., Strub, J.M., Moniatte, M., Nullans, G., Poteur, L., Hubert, P., Van Dorsselaer, A., Aunis, D., and Metz-Boutigue, M.H., 1996, The C-terminal bisphosphorylated proenkephalin-A (209–237) peptide from adrenal medullary chromaffin granules possesses antibacterial activity. Eur. J. Biochem. 235: 516–525.

    Article  PubMed  CAS  Google Scholar 

  • Goumon, Y., Lugardon, K., Kieffer, B., Lefèvre, J.F., Van Dorsselaer, A., Aunis, D., and Metz-Boutigue, M.H., 1998, Characterization of antibacterial COOH-terminal proenkephalin-A-derived peptides (PEAP) in infectious fluids. Importance of enkelytin, the antibacterial PEAP209-237 secreted by stimulated chromaffin cells. J. Biol. Chem. 273: 29847–29856.

    Article  PubMed  CAS  Google Scholar 

  • Harder, J., Bartels, J., Christophers, E., and Schroder, J.M., 1997, A peptide antibiotic from human skin. Nature (London) 387: 867.

    Article  CAS  Google Scholar 

  • Harwig, S.S.L., Swiderek, K.M., Kokriakov, V.N., Tan, L., Lee, T.D., Panyutich, E.A., Aleshina, G.M., Shamova, O.V., and Lehrer, R.I., 1994, Gallinacins: cysteine-rich antimicrobial peptides of chicken leukocytes. FEBS Lett. 342: 281–285.

    Article  PubMed  CAS  Google Scholar 

  • Helle, K.B., 1990, Chromogranins: universal proteins in secretory organelles from paramecium to man. Neurochem. Int. 17: 165–175.

    Article  CAS  Google Scholar 

  • Helle, K.B., Marley, P.D., Hogue-Angeletti, R., Galindo, E., Aunis, D., Small, D.H., and Livett, B.G., 1993, Chromogranin A: secretion of processed products from the stimulated retrogradely perfused bovine adrenal gland. J. Neuroendocrinol. 5: 413–420.

    PubMed  CAS  Google Scholar 

  • Hoffmann, J.A., 1995, Innate immunity ofinsects. Curr. Opin. Immunol. 7: 4–10.

    Article  PubMed  CAS  Google Scholar 

  • Hoffmann, J.A., Reichhart, J.M., and Hetru, C., 1996, Innate immunity in higher insects. Curr. Opin. Immunol. 8: 8–13.

    Article  PubMed  CAS  Google Scholar 

  • Hoffmann, J.A., Kafatos, F.C., Janeway, C.A. Jr., and Ezkowitz, R.A.B., 1999, Phylogenetic perspectives in innate immunity. Science 284: 1313–1318.

    Article  PubMed  CAS  Google Scholar 

  • Iacangelo, A., Fischer-Colbrie, R., Koller, K.J., Brownstein, M.J., and Eiden, L.E., 1988a, The sequence of porcine chromogranin A messenger RNA demonstrates chromogranin A can serve as the precursor of the biologically active hormone, pancreastatin. Endocrinology 122: 2339–2341.

    PubMed  CAS  Google Scholar 

  • Iacangelo, A., Okayama, H., and Eiden, L.E., 1988b, Primary structure of rat chromogranin A and distribution of its mRNA. FEBS Lett. 227: 115–121.

    Article  PubMed  CAS  Google Scholar 

  • Kieffer, B., Goumon, Y., Dillmann, B., Lefevre, J.F., Aunis, D., and Metz-Boutigue, M.H., 1998, Solution conformation of the synthetic bovine proenkephalin-A209-237 by 1HNMR spectroscopy. J. Biol. Chem. 273: 33517–33523.

    Article  PubMed  CAS  Google Scholar 

  • Kojima, K., Kilpatrick, D.L., Stem, A.S., Jones, B.N., and Udenfriend, S., 1982, Proenkephalin: a general pathway for enkephalin biosynthesis in animal tissues. Arch. Biochem. Biophys. 215: 638–643.

    Article  PubMed  CAS  Google Scholar 

  • Konecki, D., Benedum, U., Gerdes, H.H., and Huttner, W., 1987, The primary structure of chromogranin A and pancreastatin. J. Biol. Chem. 262: 17026–17030.

    PubMed  CAS  Google Scholar 

  • Lee, I.H., Zhao, C., Cho, Y., Harwig, S.S., Cooper, E.L., and Lehrer, R.I., 1997, Clavanins, alpha-helical antimicrobial peptides from tunicate hemocytes. FEBS Lett. 400: 158–162.

    Article  PubMed  CAS  Google Scholar 

  • Lehrer, R.I., Lichtenstein, A.K., and Ganz, T., 1993, Defensins: antimicrobial and cytotoxic peptides of mammalian cells. Annu. Rev. Immunol. 11: 105–126.

    Article  PubMed  CAS  Google Scholar 

  • Liang, F., Dillen, L., Zhang, X.Y., Coen, E.P., Hogue-Angeletti, R., Claeys, M., and De Potter, W. P., 1995, Vasostatins, N-terminal products of chromogranin A are released from the stimulated calf spleen in vitro. Acta Physiol. Scand. 155: 3–30.

    Article  Google Scholar 

  • Linner, K.M., Beyer, H.S., and Sharp, B.M., 1991, Induction of the messenger ribonucleic acid from promkephalin A in cultured murine CD4-positive thymocytes. Endocrinology 128: 717–724.

    Article  PubMed  CAS  Google Scholar 

  • Liston, D., Patey, G., Rossier, J., Verbanck, P., and Vanderhaegen, J.J., 1984, Processing of proenkephalin-A is tissue-specific. Science 225: 734–737.

    PubMed  CAS  Google Scholar 

  • Lugardon, K., Raffner, R., Goumon, Y., Corti, Y., Delmas, A., Bulet, P., Aunis, D., and Metz-Boutigue, M.H., 2000, Antibacterial and antifungal activities of vasostatin-I, the N-terminal fragment ofchromogranin A. J. Biol. Chem 275: in press.

    Google Scholar 

  • Martin, J., Prystowsky, M.B., and Hogue-Angeletti, R., 1987, Preproenkephalin mRNA in T-cells, macrophages and mast cells. J. Neurosci. Res. 18: 82–87.

    Article  PubMed  CAS  Google Scholar 

  • Mathis, J.P., and Lindberg, I., 1992, Posttranslational processing of proenkephalin-A in AtT-20 cells: evidence for cleavage at a Lys-Lys site. Endocrinology 131: 2287–2296.

    Article  PubMed  CAS  Google Scholar 

  • Metz-Boutigue, M.H., Garcia-Sablone, P., Hogue-Angeletti, R., and Aunis, D., 1993, Intracellular and extra-cellular processing of chromogranin A. Determination of cleavage sites. Eur. J. Biochem. 217: 247–257.

    Article  PubMed  CAS  Google Scholar 

  • Metz-Boutigue, M.H., Goumon, Y., Lugardon, K., Strub, J.M., and Aunis, D., 1998, Antibacterial peptides are present in chromaffin cell secretory granules. Cell. Mol. Neurobiol. 18: 249–266.

    Article  PubMed  CAS  Google Scholar 

  • Ouellette, A.J., and Selsted, M.E., 1996, Paneth cell defensins: endogenous peptide components ofintestinal host defense. FASEBJ. 10: 1280–1289.

    CAS  Google Scholar 

  • Piitsep, K., Bränden, C.I., Boman, H.G., and Normark, S., 1999, Antibacterial peptide from H. pylori. Nature (London) 398: 671–672.

    Google Scholar 

  • Rosen H., Behar, O., Abramsky, O., and Ovadia, H., 1989, Regulated expression of proenkephalin A in normal lymphocytes. J. Immunol. 143: 3703–3707.

    PubMed  CAS  Google Scholar 

  • Russell, J.P., Diamond, G., Tarver, A.P., Scanlin, T.F., and Bevins, C.L., 1996, Coordinate induction of two antibiotic genes in tracheal epithelial cells exposed to the inflammatory mediators lipopolysaccharide and tumor necrosis factor alpha. Infect. Immun. 64: 1565–1568.

    PubMed  CAS  Google Scholar 

  • Russell, J., Gee, P., Liu, S.M., and Hogue-Angeletti, R., 1994, Inhibition of parathyroid hormone secretion by amino terminal chromogranin peptides. Endocrinology 135: 337–342.

    Article  PubMed  CAS  Google Scholar 

  • Sato, F., Ishida, N., Hasegawa, T., and Mukoyama, H., 1999, DDBJ/EMBL/Genbank databases.

    Google Scholar 

  • Selsted, M.E., and Ouellette, A.J., 1995, Defensins in granules of phagocytic and non-phagocytic cells. Trends CellBiol. 5: 114–119.

    CAS  Google Scholar 

  • Simon, J.P., and Aunis, D., 1989, Biochemistry of the chromogranin A protein family. Biochem. J. 262: 1–13.

    PubMed  CAS  Google Scholar 

  • Stefano, G.B., Cadet, P., Dokun, A., and Scharrer, B., 1990, A neuroimmunoregulatory-like mechanism responding to stress in the marine bivalve Mytilus edulis. Brain Behuv. Immun. 4: 323–329.

    CAS  Google Scholar 

  • Stem, A.S., Jones, B.N., Shively, J.E., and Udenfiiend, S., 1981, Two adrenal opioid polypeptides: proposed intermediates in the processing of proenkephalin-A. Proc. Natl. Acad. Sci. USA 78: 1962–1966.

    Google Scholar 

  • Strub, J.M., Garcia-Sablone, P., Lonning, K., Taupenot, L., Hubert, P., Van Dorsselaer, A., Aunis, D., and Metz-Boutigue, M.H., 1995, Processing of chromogranin B in bovine adrenal medulla. Identification of secretolytin, the endogenous C-terminal fragment of residues 614–626 with antibacterial activity. Eur. J. Biochem. 229: 356–368.

    Article  PubMed  CAS  Google Scholar 

  • Strub, J.M., Hubert, P., Nullans, G., Aunis, D., and Metz-Boutigue, M.H., 1996a, Antibacterial activity of secretolytin, a chromogranin B derived peptide 614–626 is correlated with peptide structure. FEBS Lett. 379: 273–278.

    Article  PubMed  CAS  Google Scholar 

  • Strub, J.M., Goumon, Y., Lugardon, K., Capon, C., Lopez, M., Moniatte, M., Van Dorsselaer, A., Aunis, D., and Metz-Boutigue, M.H., 1996b, Antibacterial activity of glycosylated and phosphorylated chromogranin A-derived peptide 173–194 from bovine adrenal medullary chromaffin granules. J. Biol. Chem. 271: 28533–28540.

    PubMed  CAS  Google Scholar 

  • Taupenot, L., Remacle, J.E., Helle, K.B., Aunis, D., and Bader, M.F., 1995, Recombinant chromogranin A: expression, purification and characterization of the N-terminal derived peptides. Regul. Pept. 56: 71–88.

    Article  PubMed  CAS  Google Scholar 

  • Taupenot, L., Ciesielski-Treska, J., Ulhrich, G., Chasserot-Golaz, S., Aunis, D., and Bader, M.F., 1996, Chromogranin A triggers a phenotypic transformation and the generation of NO in brain microglial cells. Neuroscience 72: 377–389.

    Article  PubMed  CAS  Google Scholar 

  • Thiele, C., and Huttner, W.B., 1998, The disulfide bonded loop of chromogranins, which is essential for sorting to secretory granules mediates homodimerization. J. Biol. Chem. 273: 1223–1231.

    Article  PubMed  CAS  Google Scholar 

  • Turquier, V., Vaudry, H., Jegou, S., and Anouar, Y., 1999, Frog chromogranin A messenger ribonucleic acid encodes three highly conserved peptides. Coordinate regulation of proopiomelanocortin and chromogranin A gene expression in the pars intermedia of the pituitary during background color adaptation. Endocrinology 140: 4104–4112.

    Article  PubMed  CAS  Google Scholar 

  • Vouldoukis, I., Shai, Y., Nicolas, P., and Mor, A., 1996, Broad spectrum antibiotic activity of the skin-PPY. FEBS Lett. 380: 237–240.

    Article  PubMed  CAS  Google Scholar 

  • Yoo, S.H., 1993, pH-dependent association of chromogranin A with secretory vesicle membrane and a putative membrane binding region of chromogranin A. Biochemistry 32: 8213–8219.

    PubMed  CAS  Google Scholar 

  • Zasloff, M., 1992, Antibiotic peptides as mediators of innate immunity. Curr. Opin. Immunol. 4: 3–7.

    Article  PubMed  CAS  Google Scholar 

  • Zhao, C.Q., Liaw, L., Lee, I.H., and Lehrer, R.I., 1997, cDNA cloning of three cecropin-like antimicrobial peptides (Styelins) from the tunicate, Styela clava. FEBS Lett. 412: 144–148.

    Article  PubMed  CAS  Google Scholar 

  • Zurawski, G., Benedik, M., Kamb, B.J., Abrams, J.S., Zurawski, S.M., and Lee, F.D., 1986, Activation of mouse T-helper cells induces abundant preproenkephalin mRNA. Science 232: 772–775.

    PubMed  CAS  Google Scholar 

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Metz-Boutigue, MH., Lugardon, K., Goumon, Y., Raffner, R., Strub, JM., Aunis, D. (2002). Antibacterial and Antifungal Peptides Derived from Chromogranins and Proenkephalin-A. In: Helle, K.B., Aunis, D. (eds) Chromogranins. Advances in Experimental Medicine and Biology, vol 482. Springer, Boston, MA. https://doi.org/10.1007/0-306-46837-9_24

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