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Purinergic Receptor-Mediated Cytotoxicity

  • J. Fred Nagelkerke
  • J. Paul Zoeteweij
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Conclusion

Mitochondria are vitally important for maintaining cellular integrity. Calcium is considered a mediator of necrosis as well as a main cause of damage in isolated mitochondria. Our results show that mitochondrial Ca2+ regulation in hepatocytes is affected when [Ca2+]i is increased; this is followed by mitochondrial dysfunction and irreversible cell injury. Mitochondrial free [Ca2+] is indicated as a key parameter in this process, and phosphate is indicated as a possible important regulator of [Ca2+]mito. In addition, we suggest a role for mitochondrial Ca2+ deposits, which present only in cells killed by high [Ca2+]i.

The mechanisms underlying Ca2+-induced mitochondrial damage are rapidly elucidated; it remains to be seen whether, in intact cells, opening of a large membranous pore, opening of small ion channels, or nonspecific mitochondrial membrane destruction is involved. Our results suggest at least a small permeability change of the mitochondrial inner membrane, allowing, for example, K+ fluxes.

Future issues include the question of what happens after mitochondrial failure. Several processes, such as extreme mitochondrial swelling, mitochondrial NAD(P)H depletion, and release of mitochondrial components must be considered as critical consequences of mitochondrial dysfunction.

Keywords

Cell Injury Mitochondrial Permeability Transition Mitochondrial Damage Mitochondrial Accumulation Lethal Injury 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Kluwer Academic Publishers 2002

Authors and Affiliations

  • J. Fred Nagelkerke
    • 1
  • J. Paul Zoeteweij
    • 1
  1. 1.Department of ToxicologyLeiden-Amsterdam Center for Drug Research, Sylvius LaboratoriesLeidenThe Netherlands

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