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Confocal Microscopy of Mitochondrial Function in Living Cells

  • John J. Lemasters
  • Ting Qian
  • Donna R. Trollinger
  • Wayne E. Cascio
  • Hisayuki Ohata
  • Anna-Liisa Nieminen
Chapter
  • 171 Downloads

Conclusion

Confocal microscopy is an essential tool for studying the physiology and pathophysiology of mitochondria within single living cells. As more parameter-indicating fluorophores are discovered, the usefulness of confocal microscopy will only increase. Uniquely, confocal microscopy permits observation of ΔΨ, pH, Ca2+ oxygen free radical generation, and membrane permeability in single mitochondria of living cells. In the future, confocal microscopy should provide new insights concerning the role of mitochondria in health and disease.

Keywords

Confocal Microscopy Mitochondrial Permeability Transition Axial Resolution Calcein Fluorescence Pinhole Diameter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • John J. Lemasters
    • 1
  • Ting Qian
    • 1
  • Donna R. Trollinger
    • 2
  • Wayne E. Cascio
    • 3
  • Hisayuki Ohata
    • 4
  • Anna-Liisa Nieminen
    • 5
  1. 1.Department of Cell Biology and AnatomyUniversity of North Carolina at Chapel HillChapel Hill
  2. 2.Department of Molecular and Cell BiologyUniversity of California at DavisDavis
  3. 3.Department of Medicine, School of MedicineUniversity of North Carolina at Chapel HillChapel Hill
  4. 4.Department of Pharmacology, School of Pharmaceutical SciencesShowa UniversityTokyoJapan
  5. 5.Department of Anatomy, School of MedicineCase Western Reserve UniversityCleveland

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