Abstract
Endometriosis, a benign gynecologic disorder, occurs in about 10% of women in reproductive age and in up to 50 % of women with infertility. The basic etiologic factors causing this disease are unknown as yet. Matrix metalloproteinases (MMP) are involved in degradation of the extracellular matrix (ECM). Their proteolytic activity is regulated by tissue inhibitors of metalloproteinases (TIMPs). Tumor necrosis factor-α converting enzyme (TACE) is a membrane-bound disintegrin metalloproteinase that processes the membrane-associated cytokine proTNF-α to its mature soluble form, TNF-α induces the secretion of several MMPs. In order to study the expression of MMP-1, -2, -3 and -9, TIMP-1 and -2, TACE and TNF-α in endometrium and endometriotic tissue, we investigated formalin-fixed paraffin sections of endometriotic tissues and normal endometrium withimmunohistochemical techniques and in situ hybridisation. Furthermore, quantitative PCR was used for quantification of TACE-mRNA in fresh tissue. We found in this study significant higher protein expression of MMP-1 and TACE and significant lower protein expression of TIMP-1 and -2 in endometriotic tissue compared to endometrium. This data may suggest that high TACE expression causes the increased conversion of membrane-bound proTNF-α into its soluble form, which stimulates the increased secretion of MMP-1. The simultaneous deficiency of TIMP-1 and -2 in endometriotic tissuesuppose an additional proteinase inhibitor imbalance in endometriosis.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Black, R.A. et. al., 1997, A metalloproteinase disintegrin that releases tumour-necrosis factoralpha from cells. Nature 385: 729–733.
Bode, W. et al., 1999, Structural properties of matrix metalloproteinases. Cel. Mol. Life Sci. 55: 639–652.
Gomez, D.E. et. al., 1997, Tissue inhibitors of metalloproteinases: structure, regulation and biological functions. Eur. J. Cell Biol. 74: 111–122.
Mauviel, A., 1993, Cytokineregulation of metalloproteinase gene expression. J. Cell. Biochem. 53: 288–295.
Nagase H., 1994, Matrix metalloproteinases. A mini-review. Contrib. Nephrol. 107: 85–93.
Rawdanowicz, T.J., et. al., 1994, Matrix metalloproteinase production by cultured human endometrial stromal cells: identification of interstitial collagenase, gelatinase-A, gelatinase-B, and stromelysin-1 and their differential regulation by interleukin-1 alpha and tumor necrosis factor-alpha. J. Clin. Endocrinol. Metab. 79: 530–536.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Kluwer Academic Publishers
About this chapter
Cite this chapter
Gottschal, C. et al. (2002). Matrix Metalloproteinases and Tace Play A Role in The Pathogenesis of Endometriosis. In: Langner, J., Ansorge, S. (eds) Cellular Peptidases in Immune Functions and Diseases 2. Advances in Experimental Medicine and Biology, vol 477. Springer, Boston, MA. https://doi.org/10.1007/0-306-46826-3_49
Download citation
DOI: https://doi.org/10.1007/0-306-46826-3_49
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-46383-9
Online ISBN: 978-0-306-46826-1
eBook Packages: Springer Book Archive