Rapid Method for Detection of Aflatoxin Presence in Groundnut by Bioanalyser

  • S. Janaki alias PriyaEmail author
  • Anurag Chathurvedi
Conference paper
Part of the Springer Proceedings in Energy book series (SPE)


Mycotoxins are highly toxic secondary metabolic products of molds, which affects a twenty-five percentage of the world’s food crops, including many foodstuffs and animal feed. Aflatoxins are potent hepatotoxic, mutagenic, immunosuppressive, and carcinogenic toxins. Analysis of aflatoxins forms an important tool in the control strategy, as these toxicants can never be removed hundred percentage from the food supply. Earlier aflatoxin detection is critical to prevent this toxin from entering the food chain and improve food safety. Hence, this present study was taken up to develop a PCR-based method for identification of aflatoxin in groundnut samples. Groundnut samples were checked the presence of the aflQ gene. PCR assay revealed the presence of aflatoxin producing aflQ gene in 12 out of 55 samples tested. The occurrence of aflatoxin in the groundnut samples was confirmed by LC-MS analysis.


Mycotoxins Genes LC-MS Aflatoxin Groundnut 


  1. 1.
    Arya A, Perelló AE (2010) Management of fungal plant pathogens. CAB International, Oxfordshire, pp 14–27CrossRefGoogle Scholar
  2. 2.
    Galvano F, Galofaro V, Ritieni A, Bognanno M, De Angelis A, Galvano G (2001) Survey of the occurrence of Aflatoxin M1 in dairy products marketed in Italy: second year of observation. Food Addit Contam 18(7):644–646CrossRefGoogle Scholar
  3. 3.
    Blount WP (1961) Turkey “X” disease. Turkeys 9:52, 55–58, 61, 77–91Google Scholar
  4. 4.
    Burlingame B, Pinerio M (2007) The essential balance: risks and benefits in food safety and quality. J Food Compos Anal 20(3–4):139–146CrossRefGoogle Scholar
  5. 5.
    Machida M, Gomi K (eds) (2010) Aspergillus: molecular biology and genomics. Caister Academic Press, NorfolkGoogle Scholar
  6. 6.
    Fu Z, Huang X, Min S (2008) Rapid determination of aflatoxins in corn and peanuts. J Chromatogr A 1209(1–2):271–274CrossRefGoogle Scholar
  7. 7.
    WHO Food Additive Series 40. Rome: World Health Organization—WHO (1998) Safety evaluation of certain food additives and contaminantsGoogle Scholar
  8. 8.
    FSSAI, Food safety and standards (contaminants, toxins and residues) regulations (2011) F. No. 2-15015/30/2010. New Delhi food safety and standards authority of India. Ministry of Health and Family WelfareGoogle Scholar
  9. 9.
    Wu (2010) The global burden of disease caused by foodborne aflatoxin. WHO Commissioned Report, Foodborne Disease Burden Epidemiology Reference Group (FERG)Google Scholar
  10. 10.
    CAST (2003) Mycotoxins: risks in plant, animal and human systems. Task force report no. 139Google Scholar
  11. 11.
    Wild CP, Gong YY (2010) Mycotoxins and human disease: a largely ignored global health issue. Carcinogenesis 31:71–82CrossRefGoogle Scholar
  12. 12.
    Turner NW, Subrahmanyam S, Piletsky SA (2009) Analytical methods for determination of mycotoxins. Anal Chim Acta 632(2):168–180CrossRefGoogle Scholar
  13. 13.
    Zheng MZ, Richard JL, Binder J (2006) A review of rapid methods for the analysis of mycotoxins. Mycopathologia 161(5):261–273CrossRefGoogle Scholar
  14. 14.
    Uraguchi KM, Yamazaki M (2005) Toxicology: HPLC biochemistry and pathology of mycotoxins. Halsted Press, Japan, pp 1–278Google Scholar
  15. 15.
    Sweetman GMA, Shuker DEG, Glover RP, Farmer PB (1998) Mass spectrometry in carcinogenesis research. Adv Mass Spectrom 14:343–376Google Scholar
  16. 16.
    Zöllner P, Helm BM (2006) Trace mycotoxin analysis in complex biological and food matrices by liquid chromatography–atmospheric pressure ionisation mass spectrometry. J Chromatogr A 1136:123–169CrossRefGoogle Scholar
  17. 17.
    Nguyen MT, Tozlovanu M, Tran TL, Leszkowicz AP (2007) Occurrence of aflatoxin B2, citrinin and ochratoxin A in rice in five provinces of the central region of Vietnam. Food Chem 205:42–47CrossRefGoogle Scholar
  18. 18.
    Pasquali M, Giraud F, Brochot C, Cocco E, Hoffmann L, Bohn T (2010) Genetic Fusarium Chemotyping as a useful tool for predicting nivalenol contamination in winter wheat. Int J Food Microbiol 137:246–253CrossRefGoogle Scholar
  19. 19.
    Chen SC, Halliday CL, Meyer W (2002) A review of nucleic acid-based diagnostic tests for systemic mycoses with an emphasis on polymerase chain reaction-based assays. Med Mycol 40:333–357CrossRefGoogle Scholar
  20. 20.
    Niessen L (2008) PCR-based diagnosis and quantification of mycotoxin-producing fungi. Adv Food Nutr Res 54:81–138CrossRefGoogle Scholar
  21. 21.
    Haughland RA, Varma MM, Wymer LJ, Vesper SJ (2004) Quantitative PCR analysis of selected Aspergillus, Penicillium and Paecilomyces species. Syst Appl Microbiol 27:198–210CrossRefGoogle Scholar
  22. 22.
    Edwards SG, O’Callaghan J, Dobson ADW (2002) PCR-based detection and quantification of mycotoxigenic fungi. Mycol Res 106:1005–1025CrossRefGoogle Scholar

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© Springer Nature Singapore Pte Ltd. 2020

Authors and Affiliations

  1. 1.Department of Foods and NutritionAcharya N. G. Ranga Agricultural UniversityHyderabadIndia

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