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Rapid Method for Detection of Aflatoxin Presence in Groundnut by Bioanalyser

  • S. Janaki alias PriyaEmail author
  • Anurag Chathurvedi
Conference paper
  • 5 Downloads
Part of the Springer Proceedings in Energy book series (SPE)

Abstract

Mycotoxins are highly toxic secondary metabolic products of molds, which affects a twenty-five percentage of the world’s food crops, including many foodstuffs and animal feed. Aflatoxins are potent hepatotoxic, mutagenic, immunosuppressive, and carcinogenic toxins. Analysis of aflatoxins forms an important tool in the control strategy, as these toxicants can never be removed hundred percentage from the food supply. Earlier aflatoxin detection is critical to prevent this toxin from entering the food chain and improve food safety. Hence, this present study was taken up to develop a PCR-based method for identification of aflatoxin in groundnut samples. Groundnut samples were checked the presence of the aflQ gene. PCR assay revealed the presence of aflatoxin producing aflQ gene in 12 out of 55 samples tested. The occurrence of aflatoxin in the groundnut samples was confirmed by LC-MS analysis.

Keywords

Mycotoxins Genes LC-MS Aflatoxin Groundnut 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2020

Authors and Affiliations

  1. 1.Department of Foods and NutritionAcharya N. G. Ranga Agricultural UniversityHyderabadIndia

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