Potential of Fish Gamete Cryopreservation in Conservation Programs in Bangladesh

  • Md. Rafiqul Islam Sarder


Bangladesh is enriched with aquatic biodiversity and ranked third in Asia after China and India. It has highly potential fisheries resources comprising 260 freshwater fish, 475 marine fish, 24 freshwater prawn, and 36 marine shrimp species. The country produced 4.134 million metric tons fish in the year 2017, in which open water capture fishery contributed 28.14% and aquaculture contributed 56.44%. Presently, Bangladesh ranked fifth in aquaculture production and third in open water capture fisheries in the world. Earlier, the lion share of the production came from open water capture fisheries, but the production has been reduced over the years due to environmental and human-created problems. As a result, natural recruitment of many fish species has been hampered and they became threatened. According to IUCN Bangladesh (2015), among the 260 fresh water fish species, 9 have been categorized as critically endangered, 30 as endangered, and 25 as vulnerable. Aquaculture production of the country is being increased day by day due to availability of seeds produced through hypophysation in the private and government hatcheries, but the quality of seeds has been deteriorated due to some genetics causes such as inbreeding, interspecific hybridization, and negative selection. Therefore, it is urgent to protect the endangered fish as well as to improve the quality of seeds of commercially important fish species. This can be done by applying cryopreservation techniques associated with genetic improvement program. Considering the potential of fish cryopreservation, research on sperm cryopreservation began in the Department of Fisheries Biology and Genetics, Bangladesh Agricultural University in 2002, and protocols have been developed for a number of fish species such as Indian major carps, exotic carps, catfishes, indigenous endangered fish species, barb, and tilapia. Breeding of Indian major carps using cryopreserved sperm in private hatcheries demonstrated excellent performance of cryopreserved sperm-originated seeds and broods that urged to establish cryogenic sperm bank of commercially important fish as well as endangered fish species in Bangladesh.


Fish production Decline of biodiversity Gamete cryopreservation Cryogenic sperm bank 


  1. Alam MS, Islam MS (2005) Population genetic structure of Catla catla (Hamilton) revealed by microsatellite DNA markers. Aquaculture 246:151–160CrossRefGoogle Scholar
  2. Azad MAK (2011) Cryopreservation of spermatozoa of indigenous stinging catfish (Heteropneustes fossilis, Bloch) for ex-situ conservation. MS Thesis, Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh, p 66Google Scholar
  3. DoF (2016) National Fish Week 2016 Compendium (In Bengali). Department of Fisheries, Ministry of Fisheries and Livestock, Bangladesh, p 148Google Scholar
  4. DoF (2017) Yearbook of Fisheries Statistics of Bangladesh 2016–17. Fisheries Resources Survey System (FRSS), Department of Fisheries, Director General, DoF, Bangladesh, p 129Google Scholar
  5. DoF (2018) National Fish Week 2018 Compendium (In Bengali). Department of Fisheries, Ministry of Fisheries and Livestock, Bangladesh, p 160Google Scholar
  6. Dudgeon D, Arthington AH, Gessner MO, Kawabata Z, Knowler DJ, Lévêque C, Naiman RJ, Prieur-Richard A, Soto D, Stiassny MLJ, Sullivan CA (2006) Freshwater biodiversity: importance, threats, status and conservation challenges. Biol Rev 81:163–182CrossRefPubMedGoogle Scholar
  7. FAO (2018) FAO year book. In: Fishery and aquaculture statistics. FAO, Rome, p 104Google Scholar
  8. Hansen MM, Simonsen V, Mensberg KLD, Sarder MRI, Alam MS (2006) Loss of genetic variation in hatchery-reared Indian major carps, Catla catla. J Fish Biol 69:229–241CrossRefGoogle Scholar
  9. Hossain MAR (2014) Habitat and fish diversity: Bangladesh perspective. In: Wahab MA, Shah MS, Hossain MAR, Barman BK and Hoq ME (eds) Advances in Fisheries Research in Bangladesh: I. Proc. of 5th Fisheries Conference & Research Fair 2012, Bangladesh Agricultural Research Council, Dhaka, 18–19 January 2012, pp 1–26Google Scholar
  10. Hossain MS, Sarder MRI (2009) Cryogenic freezing of silver carp spermatozoa for conservation of gene pool. Progress Agric 20(1&2):99–106Google Scholar
  11. Hossain MR, Rahman MK, Akhter JN, Sultana S (2016) Short term cryo-milt preservation of Pangasianodon hypophthalmus at the freshwater station of BFRI in Bangladesh. Asian Australas J Biosci Biotechnol 1(2):398–401Google Scholar
  12. Hossain MA, Rouf MA, Akter N, Sarder MRI, Rahman MM, Mollah MFA (2018) Quality assessment of cryopreserved sperm of mrigal, cirrhinus cirrhosus and its use in commercial seed production. J Aquac Tropics 33(3–4):187–200Google Scholar
  13. Hussain MG, Mazid MA (2001) Genetic improvement and conservation of carp species in Bangladesh. BFRI and ICLARM publication, DhakaGoogle Scholar
  14. Islam MZ (2017) Production of YY supermale of Nile tilapia (Oreochromis niloticus) and cryopreservation of sperm. PhD Dissertation, Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, MymensinghGoogle Scholar
  15. Islam MM, Noor MN, Islam AA, Sarder MRI, Islam MZ, Jahan DA (2016) Development of a sperm cryopreservation protocol of Gangetic Mystus (Mystus cavasius). Progress Agric 27(4):517–529CrossRefGoogle Scholar
  16. IUCN Bangladesh (2000) International union for conservation of nature, Bangladesh, Bangladesher Bipanno Prani. IUCN, The World Conservation Union. Bangladesh, p 294Google Scholar
  17. IUCN Bangladesh (2015) Red List of Bangladesh 5: Freshwater Fishes. International Union for Conservation of Nature, Bangladesh Country Office, Dhaka, Bangladesh, pp xvi+360Google Scholar
  18. Khan MGQ, Mollah MFA, Ahmed AU, Hasanat MA, Uddowla MH (2004) Cryopreservation of bighead carp (Aristichthys nobilis) spermatozoa for gene resource conservation. Bangladesh J Fish 28(1–2):23–37Google Scholar
  19. Khan NS, Sarder MRI, Faroque MAA, Mollah MFA (2015) Standardization of sperm cryopreservation techniques of Indian Major Carp Rohu (Labeo rohita, Hamilton 1822). Int J Fish Aquat Stud 2(6):175–181Google Scholar
  20. Moniruzzaman (2010) Cryogenic preservation of sperm of indigenous catfish Magur, Clarias batrachus for gene pool conservation. MS Thesis, Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh, p 69Google Scholar
  21. Nahiduzzaman M, Hassan MM, Khanam UH, Mamun SNA, Hossain MAR, Tiersch TR (2011) Sperm cryopreservation of the critically endangered olive barb (Sarpunti) Puntius sarana (Hamilton, 1822). Cryobiology 62:62–67CrossRefPubMedGoogle Scholar
  22. Nahiduzzaman M, Hassan MM, Roy PK, Hossain MA, Hossain MAR, Tiersch TR (2012) Sperm cryopreservation of the Indian major carp, Labeo calbasu: effects of cryoprotectants, cooling rates and thawing rates on egg fertilization. Anim Reprod Sci 136:133–138CrossRefPubMedGoogle Scholar
  23. Nazrul KMN, Sarder MRI, Tonny US (2013) Sperm cryopreservation protocol of Anabas testudineus (Bloch 1792). LAP Lambert Academic Publishing, USAGoogle Scholar
  24. Noor MN, Sarder MRI, Islam AA, Jahan DA, Islam MM (2018) Development of sperm cryopreservation technique of minor carp, bata (Labeo bata Ham. 1822) for ex-situ conservation. Bangladesh J Fish 30(2):121–133Google Scholar
  25. Parveen S, Faisal IM (2002) People versus power: the geopolitics of Kaptai Dam in Bangladesh. Int J Water Resour Dev 18:197–208CrossRefGoogle Scholar
  26. Rafiquzzaman SM, Sarder MRI, Islam MS, Sultana R (2007) Sperm cryopreservation of rohu, Labeo rohita: cryodiluents, sperm: cryodiluents ratio and cryoprotectant concentration. Bangladesh J Fish Res 11(2):141–152Google Scholar
  27. Rahman F, Sarder MRI, Rouf MA (2009) Comparison of growth performance between cryopreserved and fresh sperm-originated fry of Barbonymus gonionotus. J Bangladesh Agric Univ 7(1):145–149CrossRefGoogle Scholar
  28. Rahman MM, Ali MR, Sarder MRI, Mollah MFA, Khan NS (2016) Development of sperm cryopreservation protocol of endangered spiny eel, Mastacembelus armatus (Lacepede 1800) for ex-situ conservation. Cryobiology 73:316–323CrossRefPubMedGoogle Scholar
  29. Rashed M (2016) Production of seed of Labeo rohita (Hamilton, 1822) using cryopreserved seed and determination of their growth performance. MS Thesis, Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, MymensinghGoogle Scholar
  30. Sarder MRI, Salam MA, Shafin SM, Hussain MG, Islam MS (2005) Cryopreservation of sperm of common carp, Cyprinus carpio and silver barb, Barbonymus gonionotus for genetically improved seed production. Bangladesh J Fish Res (Special Issue) 9(1):1–3Google Scholar
  31. Sarder MRI, Rafiquzzaman SM, Islam MS, Sultana R (2006) Cryopreservation of spermatozoa of tilapia, Oreochromis niloticus L. by optimizing different factors. J Asiat Soc Bangladesh Sci 32(1):89–96Google Scholar
  32. Sarder MRI, Rafiquzzaman SM, Islam MF (2009) Cryopreservation of spermatozoa of Mrigal, Cirrhinus cirrhosus with a view to minimize inbreeding and hybridization. J Bangladesh Agric Univ 7(1):211–218CrossRefGoogle Scholar
  33. Sarder MRI, Hossain MA, Hossain MS (2010) Cryofreezing of grass carp (Ctenopharyngodon idella) spermatozoa for ex-situ conservation. Progress Agric 21(1 & 2):141–150Google Scholar
  34. Sarder MRI, Rahman AKMA, Samad MS, Nazrul KMS, Bhuiyan MK (2011) Cryopreservation of sperm of Labeo rohita (Hamilton, 1822) and its use in fertilization and hatching of eggs. Progress Agric 22(1 & 2):137Google Scholar
  35. Sarder MRI, Sarker MFM, Saha SK (2012) Cryopreservation of sperm of an indigenous endangered fish species Nandus nandus (Hamilton, 1822) for ex-situ conservation. Cryobiology 65:202–209CrossRefPubMedGoogle Scholar
  36. Sarder MRI, Saha SK, Sarker MFM (2013) Cryopreservation of sperm of an indigenous endangered fish, Pabda (Ompok pabda, Hamilton-Buchanan, 1822). N Am J Aquac 75:114–123CrossRefGoogle Scholar
  37. Sarder MRI, Islam AA, Islam MM, Noor MN, Jahan DA (2017) Cryopreservation of spermatozoa of threatened Asian striped dwarf catfish Mystus vittatus (Bloch 1794) for ex-situ conservation: an approach to poverty alleviation. Asian J Poverty Stud 3(2):157–169Google Scholar
  38. Shamsuzzaman MM, Islam MM, Tania NJ, Al-Mamun MA, Barman PP, Xu X (2017) Fisheries resources of Bangladesh: present status and future direction. Aquac Fish 2:145–156CrossRefGoogle Scholar
  39. Simonsen V, Hansen MM, Sarder MRI, Alam MS (2004) High level of hybridization in three species of Indian major carps. NAGA WorldFish Center Q 27(1 & 2):65–69Google Scholar
  40. Simonsen V, Hansen MM, Mensberg KLD, Sarder MRI, Alam MS (2005) Widespread hybridization among species of Indian major carps in hatcheries, but not in the wild. J Fish Biol 67:794–808CrossRefGoogle Scholar
  41. Sultana N (2016) Standardization of cryopreservation protocol of Catla catla spermatozoa for its gene pool conservation. MS Thesis, Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, MymensinghGoogle Scholar
  42. Sultana S, Hasan MM, Hossain MS, Islam MR, Salimullah M, Sarder MRI, Islam MS, Moniruzzaman M, Alam J (2017) Cryogenic preservation of critically endangered Cirrhinus reba fish sperm. Int J Fish Aquat Stud 5(5):334–339Google Scholar
  43. Uddowla MH, Salma U, Rahman MM, Hossain MS, Mollah MFA (2011) Effects of diluents, dilution ratios and cryoprotectants for the cryopreservation of Thai Sharpunti (Barbodes gonionotus) spermatozoa. Int J Nat Sci 1(2):44–51CrossRefGoogle Scholar
  44. Ullah AM (2018) Production of seeds using cryopreserved sperm-originated males (F1) of Labeo rohita and their growth performance. MS Thesis, Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, MymensinghGoogle Scholar
  45. World Bank (2005) The International bank for reconstruction and development saving fishes and fishers. Towards sustainable and equitable governance of the global fishing sector 1818 H. Street. Washington DC.

Copyright information

© Springer Nature Singapore Pte Ltd. 2020

Authors and Affiliations

  • Md. Rafiqul Islam Sarder
    • 1
  1. 1.Department of Fisheries Biology and GeneticsBangladesh Agricultural UniversityMymensinghBangladesh

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