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Principle and Practice of Three-Dimensional Transmission Electron Microscopy (3D-TEM)

  • Shinzo Kohjiya
  • Atsushi Kato
  • Yuko Ikeda
Chapter
  • 68 Downloads
Part of the Springer Series on Polymer and Composite Materials book series (SSPCM)

Abstract

TEM observation principle is outlined at first. In TEM, electron is irradiated onto the sample, followed by an image formation using the transmitted electron by the bright-field method. A certain amount of defocusing of the objective lens can give a beneficial phase difference between the scattered and un-scattered electron waves to produce a necessary contrast on the screen, which is the phase contrast. Secondly, the observation principle of 3D-TEM and TEM-tomography were explained. In order to construct the 3D images, we have to make use of computerized tomography. Moreover, the new developments of 3D-TEM were touched on.

Keywords

Transmission electron microscope (TEM) Bright-field observation Three-dimensional transmission electron microscope (3D-TEM) TEM-tomography 

References

  1. 1.
    M. Born, E. Wolf, Principles of Optics, 5th edn. (Pergamon, Oxford, 1975)Google Scholar
  2. 2.
    M. Tsuji, S. Kohjiya, Program. Polym. Sci. 20, 259 (1995)CrossRefGoogle Scholar
  3. 3.
    A. Saito, KENBIKYO 60, 150 (2015) (in Japanese)Google Scholar
  4. 4.
    N. Tanaka, J. Cryst. Soc. Jpn. 47, 20 (2005) (in Japanese)CrossRefGoogle Scholar
  5. 5.
    O. Scherzer, J. Appl. Phys. 20, 20 (1949)CrossRefGoogle Scholar
  6. 6.
    L. Reimer, Transmission Electron Microscopy, 4th edn. (Springer, Berlin, 1997)CrossRefGoogle Scholar
  7. 7.
    T.C. Gruber, T.W. Zerda, Rubber Chem. Technol. 67, 280 (1994)CrossRefGoogle Scholar
  8. 8.
    S. Kohjiya, A. Kato, Y. Ikeda, Program. Polym. Sci. 33, 979 (2008).CrossRefGoogle Scholar
  9. 9.
    G.E. Paalade, J. Exp. Med. 95, 285 (1952)CrossRefGoogle Scholar
  10. 10.
    K. Kato, Polym. Eng. Sci. 7, 38 (1967)CrossRefGoogle Scholar
  11. 11.
    J.S. Trent, J.I. Scheinbein, P.R. Couchanan, Macromolecules 16, 589 (1983)CrossRefGoogle Scholar
  12. 12.
    J.S. Trent, Macromolecules 17, 2930 (1984)CrossRefGoogle Scholar
  13. 13.
    M. Tsuji, M. Fujita, S. Kohjiya, Nihon Reoroji Gakkaishi 25, 193 (1997) (in Japanese)CrossRefGoogle Scholar
  14. 14.
    J. Frank (ed.), Electron Tomography: Three-Dimensional Imaging with the Transmission Electron Microscope (Plenum Press, New York, 1992)Google Scholar
  15. 15.
    J.R. Kremer, D.N. Mastronarde, J.R. McIntosh, J. Struct. Biol. 116, 71 (1996)CrossRefGoogle Scholar
  16. 16.
    K. Jinnai, Hyoumen Kagaku 28, 467 (2007) (in Japanese)Google Scholar
  17. 17.
  18. 18.
    D. Stalling, M. Westerhoff, H.-C. Hege, Amira: a highly interactive system for visual data analysis, in The Visualization Handbook, Chap. 38, ed. by C.D. Hansen, C.R. Johnson (Academic Press, San Diego, 2005)Google Scholar
  19. 19.
    S. Kohjiya, A. Kato, T. Suda, J. Shimanuki, Y. Ikeda, Polymer 47, 3298 (2006)Google Scholar
  20. 20.
    N. Kawase, M. Kato, H. Nishioka, H. Jinnai, Ultramicroscopy 107, 8 (2007)CrossRefGoogle Scholar
  21. 21.
    K. Kaneko, N. Baba, K. Jinnai, KENBIKYO 45, 109 (2010) (in Japanese)Google Scholar
  22. 22.
    R.A. Crowther, D.J. DeRosier, A. Klug, Proc. R. Soc. London 317, 319 (1970)Google Scholar
  23. 23.
    M. Radenmacher, J. Electron Microsc. Tech. 9, 359 (1988)CrossRefGoogle Scholar
  24. 24.
    H. Jinnai, Y. Shinbori, T. Kitaoka, K. Akutagawa, N. Mashita, T. Nishi, Macromolecules 40, 6758 (2007)CrossRefGoogle Scholar
  25. 25.
    M. Matsuura, Nippon Gomu Kyokaishi 71, 583 (1998) (in Japanese)CrossRefGoogle Scholar
  26. 26.
    Y. Ikeda, A. Kato, S. Kohjiya, Y. Nakajima, Rubber Science: A Modern Approach (Springer, Singapore, 2017), pp. 179–181Google Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2020

Authors and Affiliations

  • Shinzo Kohjiya
    • 1
  • Atsushi Kato
    • 2
  • Yuko Ikeda
    • 3
  1. 1.Kyoto UniversityKyotoJapan
  2. 2.Department of Automotive AnalysisNISSAN ARC, LTD.YokosukaJapan
  3. 3.Faculty of Molecular Chemistry and Engineering, Center for Rubber Science and TechnologyKyoto Institute of TechnologyKyotoJapan

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