Chapter 4 Fish Sperm Maturation, Capacitation, and Motility Activation

  • Luz M. PérezEmail author


Motility activation mechanism in fish sperm has been studied only in a few species, and there are many hypotheses about the mechanism for spermatozoa activation that need further confirmation. This review summarizes the current knowledge on sperm acquisition of capability to move, happening in the sperm duct (sperm capacitation), and the ionic fluxes related to motility activation, as well as their relation with the sperm membrane potential changes.


Teleost Ion fluxes Membrane potential 


  1. Alavi SMH, Cosson J (2006) Sperm motility in fishes. (II). Effects of ions and osmolality: a review. Cell Biol Int 30:1–14. Scholar
  2. Alavi SMH, Rodina M, Viveiros ATM, Cosson J, Gela D, Boryshpolets S, Linhart O (2009) Effects of osmolality on sperm morphology, motility and flagellar wave parameters in northern pike (Esox lucius L.). Theriogenology 72:32–43. Scholar
  3. Alavi SMH, Kozak P, Hatef A, Hamackova J, Linhart O (2010) Relationships between reproductive characteristics in male Vimba vimba L. and the effects of osmolality on sperm motility. Theriogenology 74:317–325. Scholar
  4. Alavi SMH, Gela D, Rodina M, Linhart O (2011) Roles of osmolality, calcium- potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm. Comp Biochem Physiol A Mol Integr Physiol 160:166–174. Scholar
  5. Allan JD (1995) Stream ecology. In: Structure and function of running waters. 2nd edn. Springer, The Netherlands. xiv, 436 ppGoogle Scholar
  6. Asturiano JF, Pérez L, Garzón DL, Marco-Jiménez F, Peñaranda DS, Vicente JS, Jover M (2004) Physio-chemical characteristics of seminal plasma and development of media and methods for the cryopreservation of European eel sperm. Fish Physiol Biochem 30:283–293. Scholar
  7. Baynes SM, Scott AP, Dawson AP (1981) Rainbow trout, Salmo gairdnerii Richardson, spermatozoa: effects of cations and pH on motility. J Fish Biol 19(3):259–267. Scholar
  8. Billard R, Cosson J, Crim L (1993) Motility of fresh and aged halibut sperm. Aquat Living Resour 6:67–75. Scholar
  9. Boitano S, Omoto CK (1991) Membrane hyperpolarization activates trout sperm without an increase in intracellular pH. J Cell Sci 98:343–349PubMedGoogle Scholar
  10. Bondarenko O, Dzyuba B, Cosson J, Gunes Y, Prokopchuk G, Psenicka M, Linhart O (2013) Volume changes during the motility period of fish spermatozoa: interspecies differences. Theriogenology 79:872–881. Scholar
  11. Cai X, Clapham DE (2008) Evolutionary genomics reveals lineage-specific gene loss and rapid evolution of a sperm-specific ion channel complex: CatSpers and CatSperb. PLoS One 3(10):e3569. Scholar
  12. Caldwell RA, Clemo HF, Baumgarten CM (1998) Using gadolinium to identify stretch-activated channels: technical considerations. Am J Physiol 275(2 Pt 1):C619–C621. Scholar
  13. Cerdá J, Chauvigné F, Finn RN (2017) The physiological role and regulation of aquaporins in teleost germ cells. Adv Exp Med Biol 969:149–171. Scholar
  14. Cherr GN, Morisawa M, Vines CA, Yoshida K, Smith EH, Matsubara T, Pillai MC, Griffin FJ, Yanagimachi R (2008) Two egg-derived molecules in sperm motility initiation and fertilization in the Pacific herring (Clupea pallasi). Int J Dev Biol 52(5–6):743–752. Scholar
  15. Ciereszko A (2008) Chemical composition of seminal plasma and its physiological relationship with sperm motility, fertilizing capacity and cryopreservation in fish. In: Alavi SMH, Cosson JJ, Coward K, Rafiee G (eds) Fish spermatology. Alpha Science International, Oxford, pp 215–240Google Scholar
  16. Ciereszko A, Glogowski J, Dabrowski K (2000) Biochemical characteristics of seminal plasma and spermatozoa of freshwater fishes. In: Tiersch TR, Mazik PM (eds) Cryopreservation in aquatic species. World Aquaculture Society, Louisiana, pp 20–48Google Scholar
  17. Cosson J (2004) The ionic and osmotic factors controlling motility of fish spermatozoa. Aquac Int 12(1):69–85. Scholar
  18. Cosson MP, Billard R, Letellier L (1989) Rise of internal Ca2+ accompanies the initiation of trout sperm motility. Cell Motil Cytoskeleton 14:424–434. Scholar
  19. Cosson J, Groison A, Suquet M, Fauvel C (2008a) Motility characteristics of spermatozoa in cod (Gadus morhua) and hake (Merluccius merluccius). Cybium 32:176–177Google Scholar
  20. Cosson J, Groison A, Suquet M, Fauvel C, Dreanno C, Billard R (2008b) Studying sperm motility in marine fish: an overview on the state of the art. J Appl Ichthyol 24:460–486. Scholar
  21. Darszon A, Guerrero A, Galindo BE, Nishigaki T, Wood CD (2004) Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility. Int J Dev Biol 52:595–606. Scholar
  22. Detweiler C, Thomas P (1998) Role of ions and ion channels in the regulation of Atlantic croaker sperm motility. J Exp Zool 281:139–148.<139::AID-JEZ8>3.0.CO;2-PCrossRefPubMedGoogle Scholar
  23. Dreanno C, Cosson J, Suquet M, Cibert C, Fauvel C, Dorange G, Billard R (1999) Effects of osmolality, morphology perturbations and intracellular nucleotide content during the movement of sea bass (Dicentrarchus labrax) spermatozoa. J Reprod Fertil 116:113–125. Scholar
  24. Eckert R, Randall D (1989) Animal physiology, mechanisms and adaptations, 3rd edn. Interamericana-McGraw-Hill Ed, Madrid. 683 ppGoogle Scholar
  25. Fechner S, Alvarez L, Bönigk W, Müller A, Berger T, Pascal R, Trötschel C, Poetsch A, Stolting G, Siegfried KR, Kremer E, Seifert R, Kaupp UB (2015) A K+-selective CNG channel orchestrates Ca2+ signalling in zebrafish sperm. eLife 4:e07624. Scholar
  26. Gallego V, Pérez L, Asturiano JF, Yoshida M (2013) Study of pufferfish (Takifugu niphobles) sperm: development of methods for short-term storage, effects of different activation media and role of intracellular changes in Ca2+ and K+ in the initiation of sperm motility. Aquaculture 414–415:82–91. Scholar
  27. Gallego V, Martínez-Pastor F, Mazzeo I, Peñaranda DS, Herráez MP, Asturiano JF, Pérez L (2014) Intracellular changes in Ca2+ , K+ and pH after sperm motility activation in the European eel (Anguilla anguilla): preliminary results. Aquaculture 418–419:155–158. Scholar
  28. Gatti JL, Billard R, Christen R (1990) Ionic regulation of the plasma membrane potential of rainbow trout (Salmo gairdneri) spermatozoa: role in the initiation of sperm motility. J Cell Physiol 143:546–554. Scholar
  29. Gonçalves ACS, Nascimento AF, Costa AC, Leal MC, Viveiros ATM (2013) Initiation and suppression of sperm motility isosmolality-dependent in two south American fish species: streaked prochilod (Prochilodus lineatus) and Piracanjuba (Brycon orbignyanus). Anim Reprod 10:62–70Google Scholar
  30. He S, Jenkins-Keeran K, Woods LC (2004) Activation of sperm motility in striped bass via a cAMP-independent pathway. Theriogenology 61(7–8):1487–1498. Scholar
  31. Kho KH, Tanimoto S, Inaba K, Oka Y, Morisawa M (2001) Transmembrane cell signaling for the initiation of trout sperm motility: roles of ion channels and membrane hyperpolarization for cyclic AMP synthesis. Zool Sci 18:919–928CrossRefGoogle Scholar
  32. Kho KH, Morisawa M, Choi KS (2003) Membrane hyperpolarization increases cAMP to induce the initiation of sperm motility in salmonid fishes, rainbow trout and masu salmon. J Microbiol Biotechnol 13:833–840Google Scholar
  33. Kirichok Y, Navarro B, Clapham DE (2006) Whole-cell patch-clamp measurements of spermatozoa reveal an alkaline-activated Ca2+ channel. Nature 439:737–740. Scholar
  34. Koya Y, Munehara H, Takano K, Takahashi H (1993) Effects of extracellular environments on the motility of spermatozoa in several marine sculpins with internal gametic association. Comp Biochem Physiol 106:25–29. Scholar
  35. Koya Y, Munehara H, Takano K (2002) Sperm storage and motility in the ovary of the marine sculpin Alcichtys alcicornis (Teleostei: Scorpaeniformes). J Exp Zool 292:45–155. Scholar
  36. Krasznai Z, Márián T, Balkay L, Gáspár R, Trón L (1995) Potassium channel regulate hypo-osmotic shock induced motility of common carp (Cyprinus carpio) sperm. Aquaculture 125:123–128. Scholar
  37. Krasznai Z, Márián T, Izumi H, Damjanovich S, Balkay L, Trón L, Morisawa M (2000) Membrane hyperpolarization removes inactivation of Ca2+ channels, leading to Ca2+ influx and subsequent initiation of sperm motility in the common carp. Proc Natl Acad Sci U S A 97:2052–2057. Scholar
  38. Krasznai Z, Morisawa M, Morisawa S, Krasznai Z, Trón L, Gáspár R (2003a) Role of ion channels and membrane potential in the initiation of carp sperm motility. Aquat Living Resour 16:445–449. Scholar
  39. Krasznai Z, Morisawa M, Krasznai ZT, Morisawa S, Inaba K, Bazsáné ZK, Rubovszky B, Bodnár B, Borsos A, Márián T (2003b) Gadolinium, a mechano-sensitive channel blocker, inhibits osmosis-initiated motility of sea- and freshwater fish sperm, but does not affect human or ascidian sperm motility. Cell Motil Cytoskeleton 55:232–243. Scholar
  40. Lahnsteiner F, Patzner RA (1998) Sperm motility of the marine teleosts Boops boops, Diplodus sargus, Mullus barbatus and Trachurus mediterraneus. J Fish Biol 52:726–742. Scholar
  41. Lahnsteiner F, Berger B, Weismann T, Patzner A (1996) Motility of spermatozoa of Alburnus alburnus (Cyprinidae) and its relationship to seminal plasma composition and sperm metabolism. Fish Physiol Biochem 15:167–179. Scholar
  42. Lee CS, Tamaru CS, Kelley CD, Moriwake A, Miyamoto GT (1992) The effect of salinity on the induction of spawning and fertilization in the stripped mullet, Mugil cephalus. Aquaculture 102:289–296. Scholar
  43. Legendre M, Alavi SMH, Dzyuba B, Linhart O, Prokopchuk G, Cochet C, Dugué R, Cosson J (2016) Adaptations of semen characteristics and sperm motility to harsh salinity: Extreme situations encountered by the euryhaline tilapia Sarotherodon melanotheron heudelotii (Dumeril, 1859). Theriogenology 86(5):1251–1267. Scholar
  44. Lin F, Liu L, Dabrowski K (1996) Characteristics of muskellunge spermatozoa I: ultrastructure of spermatozoa and biochemical composition of semen. Trans Am Fish Soc 125(2):187–195.<0187:COMSIU>2.3.CO;2CrossRefGoogle Scholar
  45. Linhart O, Walford J, Sivaloganathan B, Lam TJ (1999) Effects of osmolality and ions on the motility of stripped and testicular sperm of freshwater- and seawater-acclimated tilapia, Oreochromis mossambicus. J Fish Biol 55:1344–1358. Scholar
  46. Liu Y, Cheng H, Tiersch TR (2018) The role of alkalinization-induced Ca2+ influx in sperm motility activation of a viviparous fish Redtail Splitfin (Xenotoca eiseni). Biol Reprod 99(6):1159–1170. Scholar
  47. Majhi RK, Kumar A, Yadav M, Swain N, Kumari S, Saha A, Pradhan A, Goswami L, Saha S, Samanta L, Maity A, Nayak TK, Chattopadhyay S, Rajakuberan C, Kumar A, Goswami C (2013) Thermosensitive ion channel TRPV1 is endogenously expressed in the sperm of a fresh water teleost fish (Labeo rohita) and regulates sperm motility. Channels (Austin) 7(6):483–492. Scholar
  48. Martinez-Pastor F, Cabrita E, Soares F, Anel L, Dinis MT (2008) Multivariate cluster analysis to study motility activation of Solea senegalensis spermatozoa: a model for marine teleosts. Reproduction 135(4):449–459. Scholar
  49. Marshall WS, Bryson SE, Idler DR (1989a) Gonadotropin stimulation of K+ secretion and Na+ absorption by brook trout (Salvelinus fontinalis) sperm duct epithelium. Gen Comp Endocrinol 75(1):118–128. Scholar
  50. Marshall WS, Bryson SE, Idler DR (1989b) Control of ion transport by the sperm duct epithelium of brook trout (Salvelinus fontinalis). Fish Physiol Biochem 7(1–6):331–336. Scholar
  51. Milla S, Terrien X, Sturm A, Ibrahim F, Giton F, Fiet J, Prunet P, Le Gac F (2008) Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout Oncorhynchus mykiss spermiation: implication with 17alpha,20-beta-dihydroxy-progesterone on milt fluidity. Reprod Biol Endocrinol 6:1–13. Scholar
  52. Miura C, Miura T (2003) Molecular control mechanisms of fish spermatogenesis. Fish Physiol Biochem 28(1–4):181–186. Scholar
  53. Miura C, Miura T (2011) Analysis of spermatogenesis using an eel model. Aqua BioSci Monogr 4(4):105–129. Scholar
  54. Miura T, Yamauchi K, Takahashi H, Nagahama Y (1991) Involvement of steroid hormones in gonadotropin-induced testicular maturation in male eels (Anguilla japonica). Biomed Res 12:241–248. Scholar
  55. Miura T, Yamauchi K, Takahashi H, Nagahama Y (1992) The role of hormones in the acquisition of sperm motility in salmonid fish. J Exp Zool 261(3):359–363. Scholar
  56. Miura T, Kasugai T, Nagahama Y, Yamauchi K (1995) Acquisition of potential for sperm motility in vitro in Japanese eel (Anguilla japonica). Fish Sci 61:533–534. Scholar
  57. Miura A, Nomura K, Imaizumi H, Jinbo T, Masuda Y, Tanaka H, Ohta H (2013) Administration of 17α-hydroxyprogesterone into mature male Japanese eel reduces sperm motility by decreasing potassium ion concentrations in the seminal plasma. Aquaculture 414–415:217–223. Scholar
  58. Morisawa M (2008) Adaptation and strategy for fertilization in the sperm of teleost fish. J Appl Ichthyol 24:362–370. Scholar
  59. Morisawa S, Morisawa M (1986) Acquisition of potential for sperm motility in rainbow trout and chum salmon. J Exp Biol 126:89–96PubMedGoogle Scholar
  60. Morisawa S, Morisawa M (1988) Induction of potential for sperm motility by bicarbonate and pH in rainbow trout and chum salmon. J Exp Biol 136:13–22PubMedGoogle Scholar
  61. Morisawa M, Suzuki K (1980) Osmolality and potassium ion: their roles in initiation of sperm motility in teleosts. Science 210:1145–1147. Scholar
  62. Morisawa M, Suzuki K, Morisawa S (1983a) Effects of potassium and osmolality on spermatozoan motility of salmonid fishes. J Exp Biol 107:105–113PubMedGoogle Scholar
  63. Morisawa M, Suzuki K, Shimizu H, Morisawa S, Yasuda K (1983b) Effects of osmolality and potassium on motility of spermatozoa from freshwater cyprinid fishes. J Exp Biol 107:95–103PubMedGoogle Scholar
  64. Morita M, Takemura A, Nakajima A, Okuno M (2006) Microtubule sliding movement in tilapia sperm flagella axoneme is regulated by Ca2+/calmodulin-dependent protein phosphorylation. Cell Motil Cytoskeleton 63(8):459–470. Scholar
  65. Morita M, Takemura A, Okuno M (2003) Requirement of Ca2+ on activation of sperm motility in euryhaline tilapia Oreochromis mossambicus. J Exp Biol 206(5):913–921. Scholar
  66. Morita M, Takemura A, Okuno M (2004) Acclimation of sperm motility apparatus in seawater-acclimated euryhaline tilapia Oreochromis mossambicus. J Exp Biol 207(2):337–345. Scholar
  67. Mundt N, Spehr M, Lishko PV (2018) TRPV4 is the temperature-sensitive ion channel of human sperm. eLife 7:e35853. Scholar
  68. Munehara H, Takano K, Koya Y (1989) Internal gametic association and external fertilization in the Elkhorn sculpin, Alcichthys alcicornis. Copeia 1989(3):673–678. Scholar
  69. Navarro B, Kirichok Y, Clapham DE (2007) KSper, a pH-sensitive K current that controls sperm membrane potential. PNAS 104(18):7688–7692. Scholar
  70. Oda S, Morisawa M (1993) Rises of intracellular Ca2+ and pH mediate the initiation of sperm motility by hyperosmolality in marine teleosts. Cell Motil Cytoskeleton 25:171–178. Scholar
  71. Ohta H, Shinriki Y (1998) Changes in osmotic pressure that trigger the initiation of sperm motility in the river sculpin Cottus hangiongensis. Fish Physiol Biochem 18:29–35. Scholar
  72. Ohta H, Ikeda K, Izawa T (1997) Increases in concentrations of potassium and bicarbonate ions promote acquisition of motility in vitro by Japanese eel spermatozoa. J Exp Zool 277:171–180.;2-MCrossRefGoogle Scholar
  73. Ohta H, Kagawa H, Tanaka H, Unuma T (2001) Control by the environmental concentration of ions of the potential for motility in Japanese eel spermatozoa. Aquaculture 198:339–351. Scholar
  74. Okuno M, Morisawa M (1989) Effects of calcium on motility of rainbow trout sperm flagella demembranated with triton X-100. Cell Motil Cytoskeleton 14(2):194–200. Scholar
  75. Perchec G, Cosson MP, Cosson J, Jeulin C, Billard R (1996) Morphological and kinetic changes of carp (Cyprinus carpio) spermatozoa after initiation of motility in distilled water. Cell Motil Cytoskeleton 353:113–120.<113::AID-CM4>3.0.CO;2-BCrossRefGoogle Scholar
  76. Pérez L, Vílchez MC, Gallego V, Morini M, Peñaranda DS, Asturiano JF (2016) Role of calcium on the initiation of sperm motility in the European eel. Comp Biochem Physiol A Mol Integr Physiol 191:98–106. Scholar
  77. Plant TD (2014) TRPs in mechanosensing and volume regulation. Handb Exp Pharmacol 223:743–766. Scholar
  78. Rahman MS, Morita M, Takemura A, Takano K (2003) Hormonal changes in relation to lunar periodicity in the testis of the forktail rabbitfish, Siganus argenteus. Gen Comp Endocrinol 131:302–309. Scholar
  79. Sachs F (2010) Stretch-activated ion channels: what are they? Physiology (Bethesda) 25(1):50–56. Scholar
  80. Sachs F (2015) Mechanical transduction by ion channels: a cautionary tale. World J Neurol 5(3):74–87. Scholar
  81. Santi CM, Martínez-López P, de la Vega-Beltrán JL, Butler A, Alisio A, Darszon A, Salkoff L (2010) The SLO3 sperm-specific potassium channel plays a vital role in male fertility. FEBS Lett 584(5):1041–1046. Scholar
  82. Schulz RW, de França LR, Lareyre JJ, Le Gac F, Chiarini-García H, Nóbrega RH, Miura T (2010) Spermatogenesis in fish. Gen Comp Endocrinol 165(3):390–411. Scholar
  83. Scott AP, Baynes SM (1982) Plasma levels of sex steroids in relation to ovulation and spermiation in rainbow trout (Salmo gairdneri). In: Richter CJJ, Goos HJT (eds) Reproductive physiology of fish. Pudoc, Wageningen, pp 103–106Google Scholar
  84. Scott AP, Sumpter JP, Stacey N (2010) The role of the maturation-inducing steroid, 17,20β-dihydroxy-4-pregnen-3-one, in male fishes: a review. J Fish Biol 76:183–224. Scholar
  85. Takei GL, Mukai C, Okuno M (2012) Transient Ca2+ mobilization caused by osmotic shock initiates salmonid fish sperm motility. J Exp Biol 215(4):630–641. Scholar
  86. Takei GL, Mukai C, Okuno M (2015) Regulation of salmonid fish sperm motility by osmotic shock-induced water influx across the plasma membrane. Comp Biochem Physiol A 182:84–92. Scholar
  87. Tan W, Aizen J, Thomas P (2014) Membrane progestin receptor alpha mediates progestin-induced sperm hypermotility and increased fertilization success in southern flounder (Paralichthys lethostigma). Gen Comp Endocrinol 200:18–26. Scholar
  88. Tanaka S, Utoh T, Yamada Y, Horie N, Okamura A, Akazawa A, Mikawa N, Poka H, Kurokura H (2004) Role of sodium bicarbonate on the initiation of sperm motility in the Japanese eel. Fish Sci 70:780–787. Scholar
  89. Tanimoto S, Morisawa M (1988) Roles for potassium and calcium channels in the initiation of sperm motility in rainbow trout. Develop Growth Differ 30(2):117–124CrossRefGoogle Scholar
  90. Tanimoto S, Kudo Y, Nakazawa T, Morisawa M (1994) Implication that potassium flux and increase in intracellular calcium are necessary for the initiation of sperm motility in salmonid fishes. Mol Reprod Dev 39:409–414. Scholar
  91. Thomas P (2003) Rapid, nongenomic steroid actions initiated at the cell surface: lessons from studies with fish. Fish Physiol Biochem 28:3–12. Scholar
  92. Thomas P, Pang Y, Zhu Y, Detweiler C, Doughty K (2004) Multiple rapid progestin actions and progestin membrane receptor subtypes in fish. Steroids 69:567–573. Scholar
  93. Thomas P, Tubbs C, Garry VF (2009) Progestin functions in vertebrate gametes mediated by membrane progestin receptors (mPRs): identification of mPRα on human sperm and its association with sperm motility. Steroids 74:614–621. Scholar
  94. Vicens A, Andrade-López K, Cortez D, Gutiérrez RM, Treviño CL (2017) Premammalian origin of the sperm-specific Slo3 channel. FEBS Open Bio 7:382–390. Scholar
  95. Vílchez MC, Morini M, Peñaranda DS, Gallego V, Asturiano JF, Pérez L (2016) Sodium affects the sperm motility in the European eel. Comp Biochem Physiol A Mol Integr Physiol 198:51–58. Scholar
  96. Vílchez MC, Morini M, Peñaranda DS, Gallego V, Asturiano JF, Pérez L (2017) Role of potassium and pH on the initiation of sperm motility in the European eel. Comp Biochem Physiol A Mol Integr Physiol 203:210–219. Scholar
  97. Vines CA, Yoshida K, Griffin FJ, Pillai MC, Morisawa M, Yanagimachi R, Cherr GN (2002) Motility initiation in herring sperm is regulated by reverse sodium-calcium exchange. Proc Natl Acad Sci U S A 99(4):2026–2031. Scholar
  98. Wang Z, Crim LW (1997) Seasonal changes in the biochemistry of seminal plasma and sperm motility in the ocean pout, Macrozoarces americanus. Fish Physiol Biochem 16:77–83. Scholar
  99. Wetzel RG (2001) Limnology; lake and river ecosystems, 3rd edn. Elsevier Academic, London. 1006 ppGoogle Scholar
  100. Wilson-Leedy JG, Kanuga MK, Ingermann RL (2009) Influence of osmolality and ions on the activation and characteristics of zebrafish sperm motility. Theriogenology 71:1054–1062. Scholar
  101. Yanagimachi R (1957a) Some properties of the sperm-activating factor in the micropyle area of the herring egg. Annot Zool Japon 30:114–119Google Scholar
  102. Yanagimachi R (1957b) Studies of fertilization in Clupea pallasi. VI. Fertilization of the egg deprived of the membrane. Jpn J Ichthyol 6:41–47Google Scholar
  103. Yanagimachi R, Kanoh Y (1953) Manner of sperm entry in herring egg, with special reference to the role of calcium ions in fertilization. J Fac Sci Hokkaido Univ Ser VI Zool 11(3):487–494Google Scholar
  104. Yanagimachi R, Harumi T, Matsubara H, Yan W, Yuan S, Hirohashi N, Iida T, Yamaha E, Arai K, Vines C, Cherr GN (2017) Chemical and physical guidance of fish spermatozoa into the egg through the micropyle. Biol Reprod 96(4):780–799. Scholar
  105. Zeng X-H, Yang C, Kim ST, Lingle CJ, Xia X-M (2011) Deletion of the Slo3 gene abolishes alkalization-activated K+ current in mouse spermatozoa. Proc Natl Acad Sci U S A 108:5879–5884. Scholar
  106. Zilli L, Schiavone R, Storelli C, Vilella S (2008) Molecular mechanisms determining sperm motility initiation in two sparids (Sparus aurata and Lithognathus mormyrus). Biol Reprod 79:356–366. Scholar
  107. Zilli L, Schiavone R, Vilella S (2012) Molecular mechanism regulating axoneme activation in marine fish: a review. Int Aquat Res 4:2. Scholar
  108. Zilli L, Schiavone R, Vilella S (2017) Role of protein phosphorylation/dephosphorylation in fish sperm motility activation: state of the art and perspectives. Aquaculture 472:73–80. Scholar
  109. Zuccarelli MD, Ingermann RL (2007) Calcium-induced quiescence of sperm motility in the bluegill (Lepomis macrochirus). J Exp Zool A Ecol Genet Physiol 307(10):590–599. Scholar

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© Springer Nature Singapore Pte Ltd. 2020

Authors and Affiliations

  1. 1.Aquaculture and Biodiversity Research GroupInstitute for Animal Science and Technology, Universitat Politècnica de ValènciaValenciaSpain

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