Composites Containing Marine Biomaterials for Bone Tissue Repair

  • K. Balagangadharan
  • Harsha Rao
  • PranavKumar Shadamarshan
  • Harini Balaji
  • N. SelvamuruganEmail author
Part of the Springer Series in Biomaterials Science and Engineering book series (SSBSE, volume 14)


In recent years, a striking development has been achieved in marine biomaterials for bone tissue repair. Marine sources have proven to be non-polluting and versatile for biomedical applications. Bone tissue engineering is a promising alternative for treating bone ailments caused due to trauma and surgical intrusions. Biocomposites comprise of biodegradable and biocompatible materials and mimic the architecture of bone and support regeneration. Significant sources of marine biomaterials are fish, invertebrates, fungi, corals, etc. Bone defects are treated using marine biocomposite polymers such as chitosan, collagen, alginate, gelatin, and ceramics. Chitosan is anti-microbial and bioactive; hydroxyapatite and collagen are significant constituents of bone, and alginate boosts mechanical strength and structural integrity of biocomposites. This chapter accounts for the source and types of biomaterials from marine fauna, the fabrication of biomaterials as scaffolds and their biological activity in enhancing bone repair in vitro and in vivo.


Alginate Alizarin red staining Alkaline phosphatase staining Biocomposites Bone defects Bone tissue engineering Chitosan Gelatin Hydroxyapatite (HAp) Marine sources Von Kossa staining 


  1. 1.
    Bose S, Roy M, Bandyopadhyay A (2012) Recent advances in bone tissue engineering scaffolds. Trends Biotechnol 30:546–554CrossRefGoogle Scholar
  2. 2.
    Boskey AL (2015) Bone composition: relationship to bone fragility and antiosteoporotic drug effects. Bonekey Rep 4:710CrossRefGoogle Scholar
  3. 3.
    Amini AR, Laurencin CT, Nukavarapu SP (2012) Bone tissue engineering: recent advances and challenges. Crit Rev Biomed Eng 40:363–408Google Scholar
  4. 4.
    Pisani P, Renna MD, Conversano F et al (2016) Major osteoporotic fragility fractures: risk factor updates and societal impact. World J Orthop 7:171–181CrossRefGoogle Scholar
  5. 5.
    Fardellone P, Séjourné A, Paccou J et al (2014) Bone remodelling markers in rheumatoid arthritis. Mediators Inflamm 2014:484280CrossRefGoogle Scholar
  6. 6.
    Bianco P (2014) “Mesenchymal” stem cells. Annu Rev Cell Dev Biol 30:677–704CrossRefGoogle Scholar
  7. 7.
    Schaffler MB, Cheung WY, Majeska R et al (2014) Osteocytes: master orchestrators of bone. Calcif Tissue Int 94:5–24CrossRefGoogle Scholar
  8. 8.
    Dimitriou R, Jones E, McGonagle D et al (2011) Bone regeneration: current concepts and future directions. BMC Med 9:66CrossRefGoogle Scholar
  9. 9.
    Lerner UH (2012) Osteoblasts, osteoclasts, and osteocytes: unveiling their intimate-associated responses to applied orthodontic forces. Semin Orthod 18:237–248CrossRefGoogle Scholar
  10. 10.
    Surmenev RA, Surmeneva MA, Ivanova AA (2014) Significance of calcium phosphate coatings for the enhancement of new bone osteogenesis—a review. Acta Biomater 10:557–579CrossRefGoogle Scholar
  11. 11.
    Kanczler JM, Oreffo RO (2008) Osteogenesis and angiogenesis: the potential for engineering bone. Eur Cell Mater 15:100–114CrossRefGoogle Scholar
  12. 12.
    Mackie EJ, Ahmed YA, Tatarczuch L et al (2008) Endochondral ossification: how cartilage is converted into bone in the developing skeleton. Int J Biochem Cell Biol 40:46–62CrossRefGoogle Scholar
  13. 13.
    Franz-Odendaal TA (2011) Induction and patterning of intramembranous bone. Front Biosci (Landmark Ed) 16:2734–2746CrossRefGoogle Scholar
  14. 14.
    Schlundt C, El Khassawna T, Serra A et al (2018) Macrophages in bone fracture healing: their essential role in endochondral ossification. Bone 106:78–89CrossRefGoogle Scholar
  15. 15.
    Fröhlich M, Grayson WL, Wan LQ et al (2008) Tissue engineered bone grafts: biological requirements, tissue culture and clinical relevance. Curr Stem Cell Res Ther 3:254–264CrossRefGoogle Scholar
  16. 16.
    Saranya N, Moorthi A, Saravanan S et al (2011) Chitosan and its derivatives for gene delivery. Int J Biol Macromol 48:234–238CrossRefGoogle Scholar
  17. 17.
    Swetha M, Sahithi K, Moorthi A et al (2012) Synthesis, characterization, and antimicrobial activity of nano-hydroxyapatite-zinc for bone tissue engineering applications. J Nanosci Nanotechnol 12:167–172CrossRefGoogle Scholar
  18. 18.
    García-Gareta E, Coathup MJ, Blunn GW (2015) Osteoinduction of bone grafting materials for bone repair and regeneration. Bone 81:112–121CrossRefGoogle Scholar
  19. 19.
    Sriram M, Sainitya R, Kalyanaraman V et al (2015) Biomaterials mediated microRNA delivery for bone tissue engineering. Int J Biol Macromol 74:404–412CrossRefGoogle Scholar
  20. 20.
    Saravanan S, Leena RS, Selvamurugan N (2016) Chitosan based biocomposite scaffolds for bone tissue engineering. Int J Biol Macromol 93B:1354–1365CrossRefGoogle Scholar
  21. 21.
    Balagangadharan K, Dhivya S, Selvamurugan N (2017) Chitosan based nanofibers in bone tissue engineering. Int J Biol Macromol 104B:1372–1382CrossRefGoogle Scholar
  22. 22.
    Salgado AJ, Coutinho OP, Reis RL (2004) Bone tissue engineering: state of the art and future trends. Macromol Biosci 4:743–765CrossRefGoogle Scholar
  23. 23.
    Pattnaik S, Nethala S, Tripathi A et al (2011) Chitosan scaffolds containing silicon dioxide and zirconia nano particles for bone tissue engineering. Int J Biol Macromol 49:1167–1172CrossRefGoogle Scholar
  24. 24.
    Tripathi A, Saravanan S, Pattnai S et al (2012) Bio-composite scaffolds containing chitosan/nano-hydroxyapatite/nano-copper-zinc for bone tissue engineering. Int J Biol Macromol 50:294–299CrossRefGoogle Scholar
  25. 25.
    Sowjanya JA, Singh J, Mohita T et al (2013) Biocomposite scaffolds containing chitosan/alginate/nano-silica for bone tissue engineering. Colloids Surf B Biointerfaces 109:294–300CrossRefGoogle Scholar
  26. 26.
    Fishero BA, Kohli N, Das A et al (2015) Current concepts of bone tissue engineering for craniofacial bone defect repair. Craniomaxillofac Trauma Reconstr 8:23–30Google Scholar
  27. 27.
    Kumar P, Vinitha B, Fathima G (2013) Bone grafts in dentistry. J Pharm Bioallied Sci 5:S125–S127CrossRefGoogle Scholar
  28. 28.
    Stevens MM (2008) Biomaterials for bone tissue engineering. Mater Today 11:18–25CrossRefGoogle Scholar
  29. 29.
    Sainitya R, Sriram M, Kalyanaraman V et al (2015) Scaffolds containing chitosan/carboxymethyl cellulose/mesoporous wollastonite for bone tissue engineering. Int J Biol Macromol 80:481–488CrossRefGoogle Scholar
  30. 30.
    Rao SH, Harini B, Shadamarshan RPK et al (2018) Natural and synthetic polymers/bioceramics/bioactive compounds-mediated cell signalling in bone tissue engineering. Int J Biol Macromol 110:88–96CrossRefGoogle Scholar
  31. 31.
    Srivastava S, Bankar R, Roy P (2013) Assessment of the role of flavonoids for inducing osteoblast differentiation in isolated mouse bone marrow derived mesenchymal stem cells. Phytomedicine 20:683–690CrossRefGoogle Scholar
  32. 32.
    Leena RS, Vairamani M, Selvamurugan N (2017) Alginate/Gelatin scaffolds incorporated with Silibinin-loaded Chitosan nanoparticles for bone formation in vitro. Colloids Surf B Biointerfaces 158:308–318CrossRefGoogle Scholar
  33. 33.
    Preethi Soundarya S, Sanjay V, Haritha Menon A et al (2018) Effects of flavonoids incorporated biological macromolecules based scaffolds in bone tissue engineering. Int J Biol Macromol 110:74–87CrossRefGoogle Scholar
  34. 34.
    Aneiros A, Garateix A (2004) Bioactive peptides from marine sources: pharmacological properties and isolation procedures. J Chromatogr B Analyt Technol Biomed Life Sci 803:41–53CrossRefGoogle Scholar
  35. 35.
    Ruocco N, Costantini S, Guariniello S et al (2016) Polysaccharides from the marine environment with pharmacological, cosmeceutical and nutraceutical potential. Molecules 21:E551CrossRefGoogle Scholar
  36. 36.
    Wang H, Fu ZM, Han CC (2014) The potential applications of marine bioactives against diabetes and obesity. Am J Marine Sci 2:1–8Google Scholar
  37. 37.
    Costa-Pinto AR, Reis RL, Neves NM (2011) Scaffolds based bone tissue engineering: the role of chitosan. Tissue Eng Part B Rev 17:331–347CrossRefGoogle Scholar
  38. 38.
    Elieh-Ali-Komi D, Hamblin MR (2016) Chitin and chitosan: production and application of versatile biomedical nanomaterials. Int J Adv Res 4:411–427Google Scholar
  39. 39.
    Kurita K (2006) Chitin and chitosan: functional biopolymers from marine crustaceans. Mar Biotechnol 8:203–226CrossRefGoogle Scholar
  40. 40.
    Khan AA, Shibata H, Kresnowati MTAP et al (2001) Production of chitin and chitosan from shrimp shells. Accessed 16 June 2018
  41. 41.
    Younes I, Rinaudo M (2015) Chitin and chitosan preparation from marine sources. Structure, properties and applications. Mar Drugs 13:1133–1174CrossRefGoogle Scholar
  42. 42.
    Bernkop-Schnürch A, Dünnhaupt S (2012) Chitosan-based drug delivery systems. Eur J Pharm Biopharm 81:463–469CrossRefGoogle Scholar
  43. 43.
    Klokkevold PR, Vandemark L, Kenney EB et al (1996) Osteogenesis enhanced by chitosan (poly-N-acetyl glucosaminoglycan) in vitro. J Periodontol 67:1170–1175CrossRefGoogle Scholar
  44. 44.
    Seol YJ, Lee JY, Park YJ et al (2004) Chitosan sponges as tissue engineering scaffolds for bone formation. Biotechnol Lett 26:1037–1041CrossRefGoogle Scholar
  45. 45.
    Boynueğri D, Ozcan G, Senel S (2009) Clinical and radiographic evaluations of chitosan gel in periodontal intraosseous defects: a pilot study. J Biomed Mater Res B Appl Biomater 90:461–466CrossRefGoogle Scholar
  46. 46.
    Venkatesan J, Kim SK (2010) Chitosan composites for bone tissue engineering—an overview. Mar Drugs 8:2252–2266CrossRefGoogle Scholar
  47. 47.
    Laurienzo P (2010) Marine polysaccharides in pharmaceutical applications: an overview. Mar Drugs 8:2435–2465CrossRefGoogle Scholar
  48. 48.
    Ehrlich H (2010) Biological materials of marine origin. Springer, New YorkCrossRefGoogle Scholar
  49. 49.
    Gómez-Guillén MC, Turnay J, Fernández-Dıaz MD et al (2002) Structural and physical properties of gelatin extracted from different marine species: a comparative study. Food Hydrocolloid 16:25–34CrossRefGoogle Scholar
  50. 50.
    Venkatesan J, Lowe B, Manivasagan P et al (2015) Isolation and characterization of nano-hydroxyapatite from salmon fish bone. Materials 8:5426–5439CrossRefGoogle Scholar
  51. 51.
    Boutinguiza M, Pou J, Comesana R et al (2012) Biological hydroxyapatite obtained from fish bones. Mat Sci Eng C - Mater 32:478–486CrossRefGoogle Scholar
  52. 52.
    Tripathi G, Basu B (2012) A porous hydroxyapatite scaffold for bone tissue engineering: physico-mechanical and biological evaluations. Ceram Int 38:341–349CrossRefGoogle Scholar
  53. 53.
    Pan LZ, He HW, Yao ZW et al (2010) Preparation and characterization of nano-hydroxy apatite/konjac glucomannan composite scaffolds. J Wuhan Univ Technol Mat Sci Ed 25:484–486CrossRefGoogle Scholar
  54. 54.
    Zhou H, Lee J (2011) Nanoscale hydroxyapatite particles for bone tissue engineering. Acta Biomater 7:2769–2781CrossRefGoogle Scholar
  55. 55.
    Dhivya S, Saravanan S, Sastry TP et al (2015) Nanohydroxyapatite-reinforced chitosan composite hydrogel for bone tissue repair in vitro and in vivo. J Nanobiotechnology 13:40CrossRefGoogle Scholar
  56. 56.
    Badii F, Howell NK (2006) Fish gelatin: structure, gelling properties and interaction with egg albumen proteins. Food Hydrocolloids 20:630–640CrossRefGoogle Scholar
  57. 57.
    Saravanan S, Chawla A, Vairamani M et al (2017) Scaffolds containing chitosan, gelatin and graphene oxide for bone tissue regeneration in vitro and in vivo. Int J Biol Macromol 104B:1975–1985CrossRefGoogle Scholar
  58. 58.
    Hoque ME, Nuge T, Yeow TK et al (2015) Gelatin based scaffolds for tissue engineering—a review. Polymer Res J 9:15–32Google Scholar
  59. 59.
    Rhein-Knudsen N, Ale MT, Meyer AS (2015) Seaweed hydrocolloid production: an update on enzyme assisted extraction and modification technologies. Mar Drugs 13:3340–3359CrossRefGoogle Scholar
  60. 60.
    Cardoso MJ, Costa RR, Mano JF (2016) Marine origin polysaccharides in drug delivery systems. Mar Drugs 14:E34CrossRefGoogle Scholar
  61. 61.
    Venkatesan J, Bhatnagar I, Manivasagan P et al (2015) Alginate composites for bone tissue engineering: a review. Int J Biol Macromol 72:269–281CrossRefGoogle Scholar
  62. 62.
    Sun J, Tan H (2013) Alginate-based biomaterials for regenerative medicine applications. Materials 6:1285–1309CrossRefGoogle Scholar
  63. 63.
    Chan BP, Leong KW (2008) Scaffolding in tissue engineering: general approaches and tissue-specific considerations. Eur Spine J 17:467–479CrossRefGoogle Scholar
  64. 64.
    Loh QL, Choong C (2013) Three-dimensional scaffolds for tissue engineering applications: role of porosity and pore size. Tissue Eng Part B Rev 19:485–502CrossRefGoogle Scholar
  65. 65.
    Mikos AG, Temenoff JS (2000) Formation of highly porous biodegradable scaffolds for tissue engineering. Electron J Biotechn 3(2).
  66. 66.
    Lu T, Li Y, Chen T (2013) Techniques for fabrication and construction of three-dimensional scaffolds for tissue engineering. Int J Nanomedicine 8:337–350CrossRefGoogle Scholar
  67. 67.
    Subia B, Kundu J, Kundu SC (2010) Biomaterial scaffold fabrication techniques for potential tissue engineering applications. In: Eberli D (ed) Tissue engineering. IntechOpen, London, pp 141–157Google Scholar
  68. 68.
    Sughanthy Siva AP, Ansari MNM (2015) A review on bone scaffold fabrication methods. Int Res J Eng Technol 2:1232–1238Google Scholar
  69. 69.
    Grey CP (2014) Tissue engineering scaffold fabrication and processing techniques to improve cellular infiltration. Dissertation, Virginia Commonwealth UniversityGoogle Scholar
  70. 70.
    Shalumon KT, Binulal NS, Selvamurugan N et al (2009) Electrospinning of carboxymethyl chitin/poly(vinyl alcohol) nanofibrous scaffolds for tissue engineering applications. Carbohyd Polym 77:863–869CrossRefGoogle Scholar
  71. 71.
    Binulal NS, Deepthy M, Selvamurugan N et al (2010) Role of nanofibrous poly(caprolactone) scaffolds in human mesenchymal stem cell attachment and spreading for in vitro bone tissue engineering–response to osteogenic regulators. Tissue Eng Part A 16:393–404CrossRefGoogle Scholar
  72. 72.
    Ibrahim HM, El- Zairy EMR (2015) Chitosan as a biomaterial—structure, properties, and electrospun nanofibers. In: Bobbarala V (ed) Concepts, compounds and the alternatives of antibacterials. IntechOpen, London, pp 81–101Google Scholar
  73. 73.
    Oftadeh R, Perez-Viloria M, Villa-Camacho JC et al (2015) Biomechanics and mechanobiology of trabecular bone: a review. J Biomech Eng 137(1):0109021–01080215CrossRefGoogle Scholar
  74. 74.
    Peter M, Ganesh N, Selvamurugan N et al (2010) Preparation and characterization of chitosan-gelatin/nanohydroxyapatite composite scaffolds for tissue engineering applications. Carbohyd Polym 80:687–694CrossRefGoogle Scholar
  75. 75.
    Guo YP, Guan JJ, Yang J et al (2015) Hybrid nanostructured hydroxyapatite-chitosan composite scaffold: bioinspired fabrication, mechanical properties and biological properties. J Mater Chem B 3:4679–4689CrossRefGoogle Scholar
  76. 76.
    Heidari F, Razavi M, Bahrololoom ME et al (2016) Mechanical properties of natural chitosan/hydroxyapatite/magnetite nanocomposites for tissue engineering applications. Mater Sci Eng C Mater Biol Appl 65:338–344CrossRefGoogle Scholar
  77. 77.
    Roy P, Sailaja RR (2015) Chitosan-nanohydroxyapatite composites: mechanical, thermal and bio-compatibility studies. Int J Biol Macromol 73:170–181CrossRefGoogle Scholar
  78. 78.
    Serra IR, Fradique R, Vallejo MC et al (2015) Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration. Mater Sci Eng C Mater Biol Appl 55:592–604CrossRefGoogle Scholar
  79. 79.
    Kavya KC, Jayakumar R, Nair S et al (2013) Fabrication and characterization of chitosan/gelatin/nSiO2 composite scaffold for bone tissue engineering. Int J Biol Macromol 59:255–263CrossRefGoogle Scholar
  80. 80.
    Lin L, Zhang H, Yao Y et al (2007) Application of image processing and finite element analysis in bionic scaffolds’ design optimizing and fabrication. In: Li K, Li X, Irwin GW, He G (eds) Life system modeling and simulation. LSMS 2007. Lecture notes in computer science, vol 4689. Springer, Heidelberg, pp 136–145Google Scholar
  81. 81.
    Li Z, Ramay HR, Hauch KD et al (2005) Chitosan-alginate hybrid scaffolds for bone tissue engineering. Biomaterials 26:3919–3928CrossRefGoogle Scholar
  82. 82.
    Levengood SKL, Zhang M (2014) Chitosan-based scaffolds for bone tissue engineering. J Mater Chem B 2:3161–3184CrossRefGoogle Scholar
  83. 83.
    Li H, Zhou CR, Zhu MY et al (2010) Preparation and characterization of homogeneous hydroxyapatite/chitosan composite scaffolds via in-situ hydration. J Biomater Nanobiotechnol 1:42–49CrossRefGoogle Scholar
  84. 84.
    Mao J, Zhao L, De Yao K et al (2003) Study of novel chitosan-gelatin artificial skin in vitro. J Biomed Mater Res A 64:301–308CrossRefGoogle Scholar
  85. 85.
    Sharma C, Dinda AK, Potdar PD et al (2016) Fabrication and characterization of novel nano-biocomposite scaffold of chitosan-gelatin-alginate-hydroxyapatite for bone tissue engineering. Mater Sci Eng C Mater Biol Appl 64:416–427CrossRefGoogle Scholar
  86. 86.
    Gaharwar AK, Schexnailder PJ, Kline BP et al (2011) Assessment of using laponite cross-linked poly(ethylene oxide) for controlled cell adhesion and mineralization. Acta Biomater 7:568–577CrossRefGoogle Scholar
  87. 87.
    Millán JL (2006) Alkaline phosphatases: structure, substrate specificity and functional relatedness to other members of a large superfamily of enzymes. Purinergic Signal 2:335–341CrossRefGoogle Scholar
  88. 88.
    Lima PA, Resende CX, Soares GD et al (2013) Preparation, characterization and biological test of 3D-scaffolds based on chitosan, fibroin and hydroxyapatite for bone tissue engineering. Mater Sci Eng C Mater Biol Appl 33:3389–3395CrossRefGoogle Scholar
  89. 89.
    Kumar JP, Lakshmi L, Jyothsna V et al (2014) Synthesis and characterization of diopside particles and their suitability along with chitosan matrix for bone tissue engineering in vitro and in vivo. J Biomed Nanotechnol 10:970–981CrossRefGoogle Scholar
  90. 90.
    Dhivya S, Keshav Narayan A, Logith Kumar R et al (2018) Proliferation and differentiation of mesenchymal stem cells on scaffolds containing chitosan, calcium polyphosphate and pigeonite for bone tissue engineering. Cell Prolif. Scholar
  91. 91.
    Wang X, Yu T, Chen G et al (2017) Preparation and characterization of a chitosan/gelatin/extracellular matrix scaffold and its application in tissue engineering. Tissue Eng Part C Methods 23:169–179CrossRefGoogle Scholar
  92. 92.
    Zhang Y, Venugopal JR, El-Turki A et al (2008) Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering. Biomaterials 29:4314–4322CrossRefGoogle Scholar
  93. 93.
    Sajesh KM, Jayakumar R, Nair SV et al (2013) Biocompatible conducting chitosan/polypyrrole-alginate composite scaffold for bone tissue engineering. Int J Biol Macromol 62:465–471CrossRefGoogle Scholar
  94. 94.
    Vimalraj S, Saravanan S, Vairamani M et al (2016) A combinatorial effect of carboxymethyl cellulose based scaffold and microRNA-15b on osteoblast differentiation. Int J Biol Macromol 93B:1457–1464CrossRefGoogle Scholar
  95. 95.
    Zhang JC, Lu HY, Lv GY et al (2010) The repair of critical-size defects with porous hydroxyapatite/polyamide nanocomposite: an experimental study in rabbit mandibles. Int J Oral Maxillofac Surg 39:469–477CrossRefGoogle Scholar
  96. 96.
    Wu HC, Wang TW, Sun JS et al (2016) Development and characterization of a bioinspired bone matrix with aligned nanocrystalline hydroxyapatite on collagen nanofibers. Materials 9:E198CrossRefGoogle Scholar
  97. 97.
    Vimalraj S, Arumugam B, Miranda PJ et al (2015) Runx2: structure, function, and phosphorylation in osteoblast differentiation. Int J Biol Macromol 78:202–208CrossRefGoogle Scholar
  98. 98.
    Risteli L, Koivula MK, Risteli J (2014) Procollagen assays in cancer. Adv Clin Chem 66:79–100CrossRefGoogle Scholar
  99. 99.
    De Toni L, Di Nisio A, Rocca MS et al (2017) Osteocalcin, a bone-derived hormone with important andrological implications. Andrology 5:664–670CrossRefGoogle Scholar
  100. 100.
    Chesnutt BM, Yuan Y, Buddington K et al (2009) Composite chitosan/nano-hydroxyapatite scaffolds induce osteocalcin production by osteoblasts in vitro and support bone formation in vivo. Tissue Eng Part A 15:2571–2579CrossRefGoogle Scholar
  101. 101.
    Lee JS, Baek SD, Venkatesan J et al (2014) In vivo study of chitosan-natural nano hydroxyapatite scaffolds for bone tissue regeneration. Int J Biol Macromol 67:360–366CrossRefGoogle Scholar
  102. 102.
    Ruan SQ, Yan L, Deng J et al (2017) Preparation of a biphase composite scaffold and its application in tissue engineering for femoral osteochondral defects in rabbits. Int Orthop 41:1899–1908CrossRefGoogle Scholar
  103. 103.
    Oryan A, Alidadi S, Bigham-Sadegh A et al (2016) Comparative study on the role of gelatin, chitosan and their combination as tissue engineered scaffolds on healing and regeneration of critical sized bone defects: an in vivo study. J Mater Sci Mater Med 27:155CrossRefGoogle Scholar
  104. 104.
    Li T, Liu ZL, Xiao M et al (2017) In vitro and in vivo studies of a gelatin/carboxymethyl chitosan/LAPONITE® composite scaffold for bone tissue engineering. RSC Advances 7:54100–54110CrossRefGoogle Scholar
  105. 105.
    Jin HH, Kim DH, Kim TW et al (2012) In vivo evaluation of porous hydroxyapatite/chitosan-alginate composite scaffolds for bone tissue engineering. Int J Biol Macromol 51:1079–1085CrossRefGoogle Scholar

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© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • K. Balagangadharan
    • 1
  • Harsha Rao
    • 1
  • PranavKumar Shadamarshan
    • 1
  • Harini Balaji
    • 1
  • N. Selvamurugan
    • 1
    Email author
  1. 1.Department of Biotechnology, School of BioengineeringSRM Institute of Science and TechnologyKattankulathurIndia

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