Biodiesel and the Potential Role of Microbial Lipases in Its Production

  • Abhishek Sharma
  • Shadiya
  • Tanvi Sharma
  • Rakesh Kumar
  • Khemraj Meena
  • Shamsher Singh Kanwar
Part of the Microorganisms for Sustainability book series (MICRO, volume 17)


Biodiesel is an alternative energy source to conventional fuel and has environmental acceptance due to its biodegradability, low toxicity and renewability. Biodiesel consists chemically of fatty acid alkyl esters and had immense prospect as a substitute liquid fuel. Biodiesel is primarily produced by trans-esterification of oils or fats with chemical catalysts or microbial lipases. The best thing about using microbial lipases as catalysts is that they have the capability to use all types of glycerides as well as free fatty acids. The main characteristics of microbial lipases are high activity, less production inhibition, less reaction time, thermostability, reusability of the immobilized enzyme and alcohol resistance. Using immobilized lipase trans-esterification of oil feedstock becomes quite feasible for biodiesel production. Various immobilization processes have to be optimized to enhance biodiesel production. The present chapter significantly deals with the basis and history of biodiesel as well as different sources of oil feedstock for biodiesel production and how microbial lipases act on these oil feedstocks for biodiesel production.


Biodiesel Fatty acid alkyl esters Microbial lipases Immobilization 



The financial support provided by DST-SRF (DST/INSPIRE Fellowship/2013/1036; IF 131077) to one of the authors (AS) in the form of a junior research fellowship by the Department of Science and Technology, New Delhi (India), is thankfully acknowledged.

Conflict of Interest

The authors declare no conflict of interest publishing this article in this book.


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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Abhishek Sharma
    • 1
  • Shadiya
    • 1
  • Tanvi Sharma
    • 2
  • Rakesh Kumar
    • 1
  • Khemraj Meena
    • 1
  • Shamsher Singh Kanwar
    • 1
  1. 1.Department of BiotechnologyHimachal Pradesh UniversityShimlaIndia
  2. 2.Department of Biotechnology and BioinformaticsJaypee University of Information TechnologySolanIndia

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