Taurine 11 pp 959-975 | Cite as

The Effect of Drug Pre-treatment on Taurine Transport at the Inner Blood-Retinal Barrier Under Variable Conditions

  • Asmita Gyawali
  • Young-Sook KangEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)


Taurine is essential for the development and function of the central nervous system, retina, and cardiovascular system. It is a naturally occurring amino acid, abundantly found in the retina. It has been shown to exhibit antioxidant, neuroprotective, and osmoregulatory functions in the retina. We used conditionally immortalized rat retinal capillary endothelial cells (TR-iBRB), in vitro, to investigate the effects of oxidative stress, high glucose (HG) and hypertonic conditions on taurine transport. TR-iBRB cells pre-treated with tumor necrosis factor alpha (TNF-α) showed a significant increase in [3H]taurine uptake rate, which, however, decreased when treated with taurine (50 mM). Addition of paeonol and propranolol to TNF-α pre-treated cells had no significant effect on [3H]taurine uptake, but the addition of 10 mM taurine caused a reduction. The uptake rate decreased under HG conditions, in contrast to that under hypertonic conditions. [3H]Taurine uptake increased with pre-incubation time. Additionally, uptake of [3H]taurine and mRNA expression of taurine transporter (TauT) decreased significantly under hypertonic and HG conditions, following pre-incubation with 10 mM taurine, 1 mM paeonol, and 0.1 mM propranolol. [3H]Taurine uptake was significantly inhibited in the presence of taurine transporters such as taurine and β-alanine. Results indicate that oxidative stress and hypertonic conditions increased taurine uptake in iBRB cell lines, whereas HG conditions reduced the uptake rate. Taurine may be useful in stabilizing the microenvironment in cells affected by oxidative stress as well as hypertonic and HG conditions. Moreover, taurine may play a key role in maintaining taurine concentrations in the taurine transporter system of retinal cells.


Taurine Inner blood-retinal barrier Hypertonic High glucose TNF-alpha Paeonol Propranolol 



Inner blood retinal barrier


Real time polymerase chain reaction


High glucose


Taurine transporter


tumor necrosis factor alpha



Our study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2011-0030074).


  1. Borck PC, Vettorazzi JF, Branco RCS, Batista TM, Santos-Silva JC, Nakanishi VY, Boschero AC, Ribeiro RA, Carneiro EM (2018) Taurine supplementation induces long-term beneficial effects on glucose homeostasis in ob/ob mice. Amino Acids 50:765–774CrossRefGoogle Scholar
  2. Chen NH, Reith ME, Quick MW (2004) Synaptic uptake and beyond: the sodium- and chloride-dependent neurotransmitter transporter family SLC6. Pflugers Arch 447:519–531CrossRefGoogle Scholar
  3. Chen W, Guo J, Xhang Y, Xhang J (2016) The beneficial effects of taurine in preventing metabolic syndrome. Food Funct 7:1849–1563CrossRefGoogle Scholar
  4. El-Sherbeny A, Naggar H, Miyauchi S, Ola MS, Maddox DM, Martin PM, Ganapathy V, Smith SB (2004) Osmoregulation of taurine transporter function and expression in retinal pigment epithelial, ganglion, and Muller cells. Invest Opthalmol Vis Sci 45:694–701CrossRefGoogle Scholar
  5. Jayanthi LD, Ramamoorthy S, Mahesh VB, Leibach FH, Ganapathy V (1995) Substrate-specific regulation of the taurine transporter in human placental choriocarcinoma cells (JAR). Biochim Biophys Acta 1235:351–360CrossRefGoogle Scholar
  6. Jung MK, Kim KY, Lee NY, Kang YS, Hwang YJ, Kim Y, Sung JJ, McKee A, Kowall N, Lee J, Ryu H (2013) Expression of taurine transporter (TauT) is modulated by heat shock factor 1(HSF1) in motor neurons of ALS. Mol Neurobiol 47:699–710CrossRefGoogle Scholar
  7. Kang YS (2006) The effect of oxidative stress on the transport of taurine in an in vitro model of the blood-brain barrier. Adv Exp Med Biol 583:291–298CrossRefGoogle Scholar
  8. Kang YS, Ohtsuki S, Takanaga H, Tomi M, Hosoya K, Terasaki T (2002) Regulation of taurine transport at the blood-brain barrier by tumor necrosis factor-alpha, taurine and hypertonicity. J Neurochem 83:1188–1195CrossRefGoogle Scholar
  9. Kang YS, Lee NY, Chung YY (2009) The change of taurine transport in variable stress states through the inner blood-retinal barrier using in vitro model. Biomol Ther 17:175–180CrossRefGoogle Scholar
  10. Kubo Y, Akanuma S, Hosoya K (2016) Impact of SLC6A transporters in physiological Taurine transport at the blood–retinal barrier and in the liver. Biol Pharm Bull 39:1903–1911CrossRefGoogle Scholar
  11. L’Amoreaux W (2012) The roles of taurine in the retina. Transworld Res Netw 37/661:215–254Google Scholar
  12. Lee NY, Kang YS (2004) The brain-to-blood efflux transport of taurine and changes the blood-brain barrier transport system by tumor necrosis factor-alpha. Brain Res 1023:141–147CrossRefGoogle Scholar
  13. Lee NY, Kang YS (2013) The effects of bisphosphonates on taurine transport in retinal capillary endothelial cells under high glucose conditions. Adv Exp Med Biol 776:59–66CrossRefGoogle Scholar
  14. Lee NY, Kang YS (2015) The changes by hypoxia inducible factor-1alpha (HIF-1α) on taurine uptake in brain capillary endothelial cells at high glucose conditions. Adv Exp Med Biol 803:501–551CrossRefGoogle Scholar
  15. Lombardini JB (1991) Taurine: retinal function. Brain Res Rev 16:151–169CrossRefGoogle Scholar
  16. Nakashima E, Pop-Busi R, Towns R, Thomas TP, Hosaka Y, Nakamura J, Greene DA, Killen PD, Schroeder J, Larkin DD, Ho YL, Stevens MJ (2005) Regulation of the human taurine transporter by oxidative stress in retinal pigmental epithelial cells stavly transformed to overexpress aldose reductase. Antioxid Redox Signal 7:1530–1542CrossRefGoogle Scholar
  17. Olson JE, Martinho E (2006) Regulation of taurinet transport in rat hippocampal neurons by hypoosmotic swelling. J Neurochem 96:1375–1389CrossRefGoogle Scholar
  18. Satsu H, Watanabe H, Arai S, Shimizu M (1997) Characterization and regulation of taurine transport in Caco-2, human intestinal cells. J Biochem 121:1082–1087CrossRefGoogle Scholar
  19. Satsu H, Miyamoto Y, Shimizu M (1999) Hypertonicity stimulates taurine uptake and transporter gene expression in Caco-2 cells. Biochemica et Biophysica 1419:89–96CrossRefGoogle Scholar
  20. Tamai I, Senmaru M, Terasayaki T, Tsuji A (1995) Na+- and Cl -dependent transport of taurine at the blood-brain barrier. Biochem Pharmacol 11:1783–1793CrossRefGoogle Scholar
  21. Tomi M, Terayama T, Isobe T, Egami F, Morito A, Kurachi M, Ohtsuki S, Kang YS, Terasaki T, Hosoya KI (2006) Function and regulation of taurine transport at the inner blood-retinal barrier. Microvasc Res 73:100–106CrossRefGoogle Scholar
  22. Tomi M, Tajima A, Tachikawa M, Hosoya KI (2008) Function of taurine transporter Slca6/TauT as a GABA transporting protein and its relevance to GABA transport in rat retinal capillary endothelial cells. Biochemica et Biophysica Acta 2008:2138–2148CrossRefGoogle Scholar
  23. Wu JY (1982) Purification and characterization of cysteic/cysteine sulfinic acids decarboxylase and L-glutamate decarboxylase in bovine brain. Proc Natl Acad Sci U S A 79:4270–4274CrossRefGoogle Scholar
  24. Yahara T, Tachikawa M, Akanuma SI, Hosoya KI (2010) Hypertonicity enhances GABA uptake by cultured rat retinal capillary endothelial cells. Drug Metab Pharmacokinet 25:611–615CrossRefGoogle Scholar

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© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.College of Pharmacy and Research Center for Cell Fate ControlSookmyung Women’s UniversitySeoulSouth Korea

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