Taurine 11 pp 923-934 | Cite as

Taurine Promotes Neuritic Growth of Dorsal Root Ganglion Cells Exposed to High Glucose in Vitro

  • Mengren Zhang
  • Inam-u-llah
  • Xiaoxia Shi
  • Pingan Wu
  • Kaixin Li
  • Raheel Suleman
  • Rana Muhammad Aadil
  • Muhammad Zubair Saleem
  • Fengyuan Piao
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1155)


Diabetic neuropathy (DN) is the most common chronic complication of DM and its major pathological changes show axonal dysfunction, atrophy and loss. However, there are few reports that taurine promotes neurite growth of dorsal root ganglion (DRG) cells. In current study, DRG neurons were exposed to high glucose (HG) with or without taurine. The neurite outgrowth of DRG neurons was observed by fluorescent immunohistochemistry method. Expression of Gap-43, Akt, phosphorylated Akt, mTOR and phosphorylated mTOR was determined by Western blot assay. Our results showed that HG significantly decreased the neurite outgrowth and expression of Gap-43 in DRG neurons. Moreover, phosphorylated levels of Akt and mTOR were downregulated in DRG neurons exposed to HG. On the contrary, taurine supplementation significantly reversed the decreased neurite outgrowth and Gap-43 expression, and the downregulated phosphorylated levels of Akt and mTOR. However, the protective effects of taurine were blocked in the presence of PI3K antagonists LY294002 or Akt antagonists Perifosine. These results indicate that taurine promotes neurite outgrowth of DRG neurons exposed to HG via activating Akt/mTOR signal pathway.


High glucose Diabetic neuropathy Taurine Dorsal root ganglion cells Neurite outgrowth 



Dorsal root ganglion


High glucose




Conflict of Interest

It is declared that there is no conflict of interest among the authors.

Research Subjects

In this study no animal was harmed or hurd.

Informed Consent

This manuscript is being submitter after consent from all authors and all authors are aware of submission.


  1. Abe N, Borson SH, Gambello MJ, Wang F, Cavalli V (2010) Mammalian target of rapamycin (mTOR) activation increases axonal growth capacity of injured peripheral nerves. J Biol Chem 285:28034–28043CrossRefGoogle Scholar
  2. Alessi DR, Andjelkovic M, Caudwell B, Cron P, Morrice N, Cohen P, Hemmings BA (1996) Mechanism of activation of protein kinase B by insulin and IGF-1. EMBO J 15:6541–6551CrossRefGoogle Scholar
  3. American Diabetes Association (2014) Diagnosis and classification of diabetes mellitus. Diabetes Care 37:81–90CrossRefGoogle Scholar
  4. Andjelkovic M, Alessi DR, Meier R, Fernandez A, Lamb NJC, Frech M, Cron P, Cohen P, Lucocq JM, Hemmings BA (1997) Role of translocation in the activation and function of protein kinase B. J Biol Chem 272:31515–31524CrossRefGoogle Scholar
  5. Asieh Hosseini, Abdollahi M (2013) Diabetic neuropathy and oxidative stress: therapeutic perspectives. Oxid Med Cell Longev 2013:1–15CrossRefGoogle Scholar
  6. Bril V (2012) Treatments for DN. J Peripher Nerv Syst 2:22–27CrossRefGoogle Scholar
  7. Chen J, Zhang ZG, Li Y, Wang Y, Wang L, Jiang H, Zhang C, Lu M, Katakowski M, Feldkamp CS, Chopp M (2012) Statins induce angiogenesis, neurogenesis, and synaptogenesis after stroke. Ann Neurol 53:743–751CrossRefGoogle Scholar
  8. Hsu YY, Tseng YT, Lo YC (2013) Berberine, a natural antidiabetes drug, attenuates glucose neurotoxicity and promotes nrf2-related neurite outgrowth. Toxicol Appl Pharmacol 272(3):787–796CrossRefGoogle Scholar
  9. Huxtable RJ (1989) Taurine in the central nervous system and the mammalian actions of taurine. Prog Neurobiol 32:471–533CrossRefGoogle Scholar
  10. Inam-u-llah FP, Aadil RM, Suleman R, Li K, Zhang M, Pingan W, Shahbaz M, Ahmed Z (2018) Ameliorative efects of taurine against diabetes: a review. Amino Acids 50(5):487–502CrossRefGoogle Scholar
  11. Kim KS, Oh DH, Kim JY, Lee BG, You JS, Chang KJ, Chung HJ, Yoo MC, Yang HI, Kang JH, Hwang YC, Ahn KJ, Chung HY, Jeong IK (2012) Taurine ameliorates hyperglycemia and dyslipidemia by reducing insulin resistance and leptin level in otsuka long-evans tokushima fatty (oletf) rats with long-term diabetes. Exp Mol Med 44(11):665–673CrossRefGoogle Scholar
  12. Kobayashi M, Zochodne DW (2018) Diabetic neuropathy and the sensory neuron: new aspects of pathogenesis and their treatment implications. J Diabetes Investig 9:1239–1254CrossRefGoogle Scholar
  13. Li F, Abatan OI, Kim H, Burnett D, Larkin D, Obrosova IG, Stevens MJ (2006) Taurine reverses neurological and neurovascular deficits in Zucker diabetic fatty rats. Neurobiol Dis 22:669–676CrossRefGoogle Scholar
  14. Li Y, Hu Z, Chen B, Bu Q, Lu W, Deng Y, Zhu R, Shao X, Hou J, Zhao J, Li H, Zhang B, Huang Y, Lv L, Zhao Y, Cen X (2012) Taurine attenuates methamphetamine-induced autophagy and apoptosis in pc12 cells through mtor signaling pathway. Toxicol Lett 215(1):1–7CrossRefGoogle Scholar
  15. Lima L (1999) Taurine and its trophic effects in the retina. Neurochem Res 24:1333–1338CrossRefGoogle Scholar
  16. Lima L, Matus P, Drujan B (1988) Taurine effect on neuritic growth from goldfish retinal explants. Int Dev Neurosci 6:417–420CrossRefGoogle Scholar
  17. Lima L, Matus P, Drujan B (1989) The interaction of substrate and taurine modulates the outgrowth from regenerating goldfish retinal explants. Int J Devl Neurosci 7:375–382CrossRefGoogle Scholar
  18. Matus P, Cubillos S, Lima L (1997) Differential effect of taurine and serotonin on outgrowth from explants or isolated cells of the retina. Int J Devl Neurosci 15:785–793CrossRefGoogle Scholar
  19. Oja SS, Kontro P (1990) Neuromodulatory and trophic actionsof taurine. Prog Clin Biol Res 351:69–76PubMedGoogle Scholar
  20. Park KK, Liu K, Hu Y, Smith PD, Wang C, Cai B, Xu B, Connolly L, Kramvis I, Sahin M, He Z (2008) Promoting axon regeneration in the adult CNS by modulation of the PTEN/mTOR pathway. Science 322:963–966CrossRefGoogle Scholar
  21. Read DE, Gorman AM (1997) Involvement of Akt in neurite outgrowth. Cell Mol Life Sci 66:2975–2984CrossRefGoogle Scholar
  22. Sarkar P, Basak P, Ghosh S, Kundu M, Sil PC (2017) Prophylactic role of taurine and its derivatives against diabetes mellitus and its related complications. Food Chem Toxicol 110:109–121CrossRefGoogle Scholar
  23. Sima AA, Nathaniel V, Bril V, McEwen TA, Greene DA (1988) Histopathological heterogeneity of neuropathy in insulindependent and non-insulin-dependent diabetes, and demonstration of axo-glial dysjunction in human diabetic neuropathy. J Clin Invest 81:349–364CrossRefGoogle Scholar
  24. Sirdah MM (2015) Protective and therapeutic effectiveness of taurine in diabetes mellitus: a rationale for antioxidant supplementation. Diabetes Metab Syndr Clin Res Rev 9(1):55–64CrossRefGoogle Scholar
  25. Sun G, Wang X, Li T, Qu S, Sun J (2018) Taurine attenuates acrylamide-induced apoptosis via a PI3K/AKT-dependent manner. Hum Exp Toxicol 37:1249–1257CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Mengren Zhang
    • 1
  • Inam-u-llah
    • 1
  • Xiaoxia Shi
    • 1
  • Pingan Wu
    • 1
  • Kaixin Li
    • 1
  • Raheel Suleman
    • 2
  • Rana Muhammad Aadil
    • 3
  • Muhammad Zubair Saleem
    • 4
  • Fengyuan Piao
    • 1
  1. 1.Department of Occupational and Environmental HealthDalian Medical UniversityDalianChina
  2. 2.Institute of Food Science and TechnologyGraduate School of Chinese Academy of Agriculture ScienceBeijingChina
  3. 3.National Institute of Food Science and TechnologyUniversity of Agriculture FaisalabadFaisalabadPakistan
  4. 4.Cancer Research CentreDalian Medical UniversityDalianChina

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